Results 171 to 180 of about 1,218,633 (316)

The influence of non-specific serum inhibitors in enzyme immune assay used for detection of anti-influenza antibodies

, 2002
Fernando Couto Motta, J. Couceiro, Maria H. Silva   +2 more
openalex   +1 more source

Diabetes‐induced vascular calcification is associated with low pyrophosphate and its oral supplementation prevents calcification in diabetic mice

FEBS Open Bio, EarlyView.
Induction of diabetes in three different mouse strains uniformly resulted in an increase in TNAP activity and a reduction in pyrophosphate (PPi) in the circulation. Inhibition of TNAP restored plasma PPi. Diabetes‐induced calcification in the media layer of the aorta was detected only in the Abcc6−/− strain, which is predisposed to ectopic ...
Krisztina Fülöp, Eszter Kozák, Natália Tőkési, Lilian Kocsis, Anikó Kelemen, Zsuzsanna Geszti, Adriána Kutás, Mariann Harangi, Ágnes Diószegi, Zsolt Rapi, József Balla, Olivier Le Saux, András Váradi, Viola Pomozi   +13 more
wiley   +1 more source

High-Throughput Fluorescence-Based Screening Assays for Tryptophan-Catabolizing Enzymes [PDF]

, 2014
Nicole Seegers, Antoon M. van Doornmalen, Joost C.M. Uitdehaag, Jos de Man, Rogier C. Buijsman, Guido J.R. Zaman   +5 more
openalex   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

An Enzyme-Linked Immunosorbent Assay (ELISA) for Measuring Human Erythrocytes Catalase

, 1987
Masaaki Kurasaki, Keizo Ito, Mika Suzuki–Kurasaki, Takeshi Saito, Tadao Makizuka, Yutaka Kojima, Kazuo Saito   +6 more
openalex   +2 more sources

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

FEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita   +3 more
wiley   +1 more source

Establishment and application of a competitive enzyme-linked immunosorbent assay differentiating PCV2 antibodies from mixture of PCV1/PCV2 antibodies in pig sera [PDF]

, 2016
Shuizhong Han, Yan Xiao, Dingding Zheng, Yanli Gu, Yajie Xuan, Yudan Jin, Wenqiang Pang, Yuxin Huang, Xiangdong Li, Junhua Deng, Kegong Tian   +10 more
openalex   +1 more source

Hands‐on protocol for preparing water‐soluble fractions from agri‐food samples for NMR‐based metabolomics analysis

FEBS Open Bio, EarlyView.
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso, Jaime Hiniesta‐Valero, Alejandra Guerra‐Castellano, Miguel A. De la Rosa, Irene Díaz‐Moreno   +4 more
wiley   +1 more source

Detection of mycobacterial antibodies in serum samples by enzyme linked immunosorbent assay

, 2011
Nath
openalex   +1 more source