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Enzyme assays

Chem. Commun., 2008
Enzyme assays are analytical tools to visualize enzyme activities. In recent years a large variety of enzyme assays have been developed to assist the discovery and optimization of industrial enzymes, in particular for "white biotechnology" where selective enzymes are used with great success for economically viable, mild and environmentally benign ...
Jean-Louis, Reymond   +2 more
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Enzyme assays

Current Opinion in Biotechnology, 1991
The past year or so has seen the development of new enzyme assays, as well as the improvement of existing ones. Assays are becoming more rapid and sensitive as a result of modifications such as amplification of the enzyme product(s). Recombinant DNA technology is now being recognized as a particularly useful tool in the search for improved assay ...
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Enzyme‐Linked Immunosorbent Assays

Current Protocols in Immunology, 1992
AbstractThis unit describes six different ELISA systems for the detection of specific antibodies, soluble antigens, or cell‐surface antigens. In all six systems, soluble reactants are removed from solution after specifically binding to solid‐phase reactants.
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Assay of enzyme effectors

Talanta, 1987
The effects of various classes of organic compounds and of metal ions on the catalytic activity of horseradish peroxidase in hydrogen peroxide-catalysed o-dianisidine oxidation and, on the activity of alkaline phosphatase in p-nitrophenyl phosphate hydrolysis have been studied.
I F, Dolmanova   +2 more
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Reporter Enzyme Assays

2003
Reporter genes code for proteins that have a unique enzymatic activity and are used to assess the transcriptional properties of DNA elements. The use of reporter genes in transgenic animals provides a rapid method for the detection of transgene expression, which is easily distinguishable from expression of the corresponding endogenous gene of the ...
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Supramolecular Tandem Enzyme Assays

Chemistry – A European Journal, 2012
AbstractWe conceptualize a novel approach towards enzyme assays based on the reversible and competitive binding of a fluorescent dye and the substrate as well as product of an enzymatic reaction to a macrocyclic host. This method was termed “supramolecular tandem assay”, and has been applied to inhibitor and activator screening, sensor array ...
Roy N, Dsouza   +2 more
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