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Clickable Enzyme-Linked Immunosorbent Assay
Biomacromolecules, 2011Click chemistry is explored as a potential cost-effective and selective immobilization method for the production of an enzyme-linked immunosorbent assay (ELISA). Coatings were formulated containing either a terminal alkyne or a bicyclo[6.1.0]non-4-yne (BCN) chemical handle, and a diagnostic peptide was subsequently immobilized onto these coatings by ...
Canalle, L.A. +7 more
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Capillary Electrophoretic Enzyme Assays
2013In the past years, capillary electrophoresis has become a frequently used technique for enzyme assays due to the high separation efficiency and versatility as well as small sample size and low consumption of chemicals. The capillary electrophoresis assays can be divided into two general categories: pre-capillary (or offline) assays and in-capillary (or
Gerhard K E, Scriba +3 more
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Enzyme-Linked Oligonucleotide Assay (ELONA)
2022Aptamers are single-stranded oligonucleotides able to recognize a target with high affinity and specificity. Aptamers are used in different diagnostics applications, highlighting, among all, variations of the traditional enzyme-linked immunosorbent assay (ELISA). In this chapter, we show the procedures for the development of two types of indirect ELONA:
Miguel, Moreno +3 more
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Enzyme-Linked Immunosorbent Assay
Journal of Immunoassay, 2000(2000). Enzyme-Linked Immunosorbent Assay. Journal of Immunoassay: Vol. 21, No. 2-3, pp. 165-209.
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Optimizing coupled enzyme assays
Analytical Biochemistry, 1979Abstract Equations are given for minimizing the cost and calculating the maximum practical rates for coupled assays when two or more coupling enzymes are involved, and a lag of a predetermined size can be tolerated. The lag caused by obligatory mutarotation of an intermediate is also calculated.
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1992
Abstract Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used to measure the rate of an enzyme-catalysed
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Abstract Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used to measure the rate of an enzyme-catalysed
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Enzyme-Coupled Assays for Proteases
Analytical Biochemistry, 1996We have developed a general strategy for assaying proteases that does not require the use of fluorogenic, chromogenic, or radiolabeled peptide substrates. The endo- or exoproteolytic hydrolysis of simple peptides can be followed spectrophotometrically by coupling the proteolytic event via enzyme-catalyzed reactions to a chromogenic redox dye.
B E, Cathers, J V, Schloss
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Automated Enzyme Assay of Antithrombin
Annals of Clinical Biochemistry: International Journal of Laboratory Medicine, 1980The conditions for the automated assay of plasma antithrombin activity over the clinically relevant range of 25–250% using the chromogenic substrate S2238 and the Gilford 3800 enzyme rate analyser are reported.
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Metal–Organic Framework-Based Enzyme Biocomposites
Chemical Reviews, 2021, Peter Wied, Francesco Carraro
exaly