Results 251 to 260 of about 1,285,868 (304)
Some of the next articles are maybe not open access.
Enzyme-Linked Oligonucleotide Assay (ELONA)
2022Aptamers are single-stranded oligonucleotides able to recognize a target with high affinity and specificity. Aptamers are used in different diagnostics applications, highlighting, among all, variations of the traditional enzyme-linked immunosorbent assay (ELISA). In this chapter, we show the procedures for the development of two types of indirect ELONA:
Miguel, Moreno +3 more
openaire +2 more sources
Enzyme-Linked Immunosorbent Assay
Journal of Immunoassay, 2000(2000). Enzyme-Linked Immunosorbent Assay. Journal of Immunoassay: Vol. 21, No. 2-3, pp. 165-209.
openaire +2 more sources
Optimizing coupled enzyme assays
Analytical Biochemistry, 1979Abstract Equations are given for minimizing the cost and calculating the maximum practical rates for coupled assays when two or more coupling enzymes are involved, and a lag of a predetermined size can be tolerated. The lag caused by obligatory mutarotation of an intermediate is also calculated.
openaire +2 more sources
1992
Abstract Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used to measure the rate of an enzyme-catalysed
openaire +1 more source
Abstract Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used to measure the rate of an enzyme-catalysed
openaire +1 more source
Enzyme-Coupled Assays for Proteases
Analytical Biochemistry, 1996We have developed a general strategy for assaying proteases that does not require the use of fluorogenic, chromogenic, or radiolabeled peptide substrates. The endo- or exoproteolytic hydrolysis of simple peptides can be followed spectrophotometrically by coupling the proteolytic event via enzyme-catalyzed reactions to a chromogenic redox dye.
B E, Cathers, J V, Schloss
openaire +2 more sources
Automated Enzyme Assay of Antithrombin
Annals of Clinical Biochemistry: International Journal of Laboratory Medicine, 1980The conditions for the automated assay of plasma antithrombin activity over the clinically relevant range of 25–250% using the chromogenic substrate S2238 and the Gilford 3800 enzyme rate analyser are reported.
openaire +2 more sources
2013
The development of enzyme assays for the detection of enzymatic activity is of prime interest in academic and industrial research, for example, in enzyme engineering and drug discovery. Therein, each application has different specifications for an ideal enzyme assay.
openaire +1 more source
The development of enzyme assays for the detection of enzymatic activity is of prime interest in academic and industrial research, for example, in enzyme engineering and drug discovery. Therein, each application has different specifications for an ideal enzyme assay.
openaire +1 more source
Loop dynamics and the evolution of enzyme activity
Nature Reviews Chemistry, 2023Marina Corbella Morató +2 more
exaly
Enzyme-Linked Immunosorbant Assay (ELBA)
2003In general, immunological methods are not very well suited for a quantitative determination of the antigen to be studied. The ELISA technique, however, can be used for a quantitative or at least semiquantitative determination of the concentration of a certain antigen. The method was first introduced by Engvall and Perlmann (1).
openaire +2 more sources