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Chem. Commun., 2008
Enzyme assays are analytical tools to visualize enzyme activities. In recent years a large variety of enzyme assays have been developed to assist the discovery and optimization of industrial enzymes, in particular for "white biotechnology" where selective enzymes are used with great success for economically viable, mild and environmentally benign ...
Jean-Louis, Reymond +2 more
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Enzyme assays are analytical tools to visualize enzyme activities. In recent years a large variety of enzyme assays have been developed to assist the discovery and optimization of industrial enzymes, in particular for "white biotechnology" where selective enzymes are used with great success for economically viable, mild and environmentally benign ...
Jean-Louis, Reymond +2 more
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Capillary Electrophoretic Enzyme Assays
Methods in molecular biology, 2013In the past years, capillary electrophoresis has become a frequently used technique for enzyme assays due to the high separation efficiency and versatility as well as small sample size and low consumption of chemicals. The capillary electrophoresis assays can be divided into two general categories: pre-capillary (or offline) assays and in-capillary (or
Gerhard K E, Scriba +3 more
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Supramolecular Tandem Enzyme Assays
Chemistry – A European Journal, 2012AbstractWe conceptualize a novel approach towards enzyme assays based on the reversible and competitive binding of a fluorescent dye and the substrate as well as product of an enzymatic reaction to a macrocyclic host. This method was termed “supramolecular tandem assay”, and has been applied to inhibitor and activator screening, sensor array ...
Roy N, Dsouza +2 more
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2013
The development of enzyme assays for the detection of enzymatic activity is of prime interest in academic and industrial research, for example, in enzyme engineering and drug discovery. Therein, each application has different specifications for an ideal enzyme assay.
A. Hennig
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The development of enzyme assays for the detection of enzymatic activity is of prime interest in academic and industrial research, for example, in enzyme engineering and drug discovery. Therein, each application has different specifications for an ideal enzyme assay.
A. Hennig
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Recent advances in histone modification and histone modifying enzyme assays
Expert Review of Molecular Diagnostics, 2019Introduction: Histone modifying enzymes (HMEs)-catalyzed histone modifications are important epigenetic markers that play critical roles in the regulation of a variety of cellular functions, especially the regulation of gene expression.
Fei Ma, Su Jiang, Chun-yang Zhang
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Current Opinion in Biotechnology, 1991
The past year or so has seen the development of new enzyme assays, as well as the improvement of existing ones. Assays are becoming more rapid and sensitive as a result of modifications such as amplification of the enzyme product(s). Recombinant DNA technology is now being recognized as a particularly useful tool in the search for improved assay ...
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The past year or so has seen the development of new enzyme assays, as well as the improvement of existing ones. Assays are becoming more rapid and sensitive as a result of modifications such as amplification of the enzyme product(s). Recombinant DNA technology is now being recognized as a particularly useful tool in the search for improved assay ...
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Angewandte Chemie, 2018
Isothermal exponential amplification techniques, such as strand-displacement amplification (SDA), rolling circle amplification (RCA), loop-mediated isothermal amplification (LAMP), nucleic acid sequence based amplification (NASBA), helicase-dependent ...
Michael S. Reid +2 more
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Isothermal exponential amplification techniques, such as strand-displacement amplification (SDA), rolling circle amplification (RCA), loop-mediated isothermal amplification (LAMP), nucleic acid sequence based amplification (NASBA), helicase-dependent ...
Michael S. Reid +2 more
semanticscholar +1 more source
Enzyme‐Linked Immunosorbent Assays
Current Protocols in Immunology, 1992AbstractThis unit describes six different ELISA systems for the detection of specific antibodies, soluble antigens, or cell‐surface antigens. In all six systems, soluble reactants are removed from solution after specifically binding to solid‐phase reactants.
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Talanta, 1987
The effects of various classes of organic compounds and of metal ions on the catalytic activity of horseradish peroxidase in hydrogen peroxide-catalysed o-dianisidine oxidation and, on the activity of alkaline phosphatase in p-nitrophenyl phosphate hydrolysis have been studied.
I F, Dolmanova +2 more
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The effects of various classes of organic compounds and of metal ions on the catalytic activity of horseradish peroxidase in hydrogen peroxide-catalysed o-dianisidine oxidation and, on the activity of alkaline phosphatase in p-nitrophenyl phosphate hydrolysis have been studied.
I F, Dolmanova +2 more
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2003
Reporter genes code for proteins that have a unique enzymatic activity and are used to assess the transcriptional properties of DNA elements. The use of reporter genes in transgenic animals provides a rapid method for the detection of transgene expression, which is easily distinguishable from expression of the corresponding endogenous gene of the ...
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Reporter genes code for proteins that have a unique enzymatic activity and are used to assess the transcriptional properties of DNA elements. The use of reporter genes in transgenic animals provides a rapid method for the detection of transgene expression, which is easily distinguishable from expression of the corresponding endogenous gene of the ...
openaire +2 more sources