A Universal Protein Ladder for Standardization of Diverse FRET Assays
Translating FRET measurements from in vitro to intracellular environments requires universal benchmarks. We present a modular protein ladder using TPR motifs to harmonize data across diverse platforms. This predictable calibration curve bridges single molecule FRET measurements, with cell based measurements including FLIM‐FRET.
Evelyn R. Smith +5 more
wiley +1 more source
Microcontact printing of choline oxidase using a polycation-functionalized zwitterionic polymer as enzyme immobilization matrix. [PDF]
Zhao M +3 more
europepmc +1 more source
A rigid‑flexible integrated CO depot is engineered by embedding rigid COFQDs into a flexible ECM gel network. The rigid framework enables high‑capacity CO storage and sonodynamic bacterial killing, while the flexible ECM gel controls sustained CO release, collectively eradicating multidrug‑resistant infection and promoting scarless healing.
Baohong Sun +13 more
wiley +1 more source
Developing Enzyme Immobilization with Fibrous Membranes: Longevity and Characterization Considerations. [PDF]
Yuan Y, Shen J, Salmon S.
europepmc +1 more source
Cancer‐Associated BCL‐2 Mutants Reveal Mechanisms Towards Venetoclax Resistance
Venetoclax (VEN) resistance in chronic lymphocytic leukemia arises from diverse BCL2 mutations. We map mechanisms contributing to VEN resistance across common BCL‐2 variants. G101V and D103Y reduce drug binding and increase sequestration of pro‐apoptotic proteins. V156D blocks VEN allosterically.
Jonas Aufdermauer +9 more
wiley +1 more source
Beyond enzyme engineering: ordered enzyme immobilization drives enhanced productivity <i>in vitro</i>. [PDF]
Wong M +6 more
europepmc +1 more source
Polyelectrolytes for Enzyme Immobilization and the Regulation of Their Properties. [PDF]
Muronetz VI, Pozdyshev DV, Semenyuk PI.
europepmc +1 more source
Probing Electrocatalyst Design for Product Selectivity in CO2 Reduction
Selective CO2 electroreduction is controlled by catalyst structure, dynamic surface reconstruction, adsorbate interactions, and reaction microenvironment. This review summarizes Cu‐ and non‐Cu‐based catalysts, single‐atom and molecular systems, degradation pathways, and operando/computational strategies for steering CO2RR toward hydrocarbons ...
Suvodeep Sen +5 more
wiley +1 more source
Mechanistic Determinants of Oriented Enzyme Immobilization from Martini Simulations. [PDF]
Jiménez-García JC +4 more
europepmc +1 more source
Microfluidic Electrochemical Glucose Biosensor with In Situ Enzyme Immobilization. [PDF]
Lokar N +3 more
europepmc +1 more source

