Results 31 to 40 of about 653,394 (261)

Recombinant Expression and Immobilization of Pantoea dispersa DJL-B Alcohol Dehydrogenase

open access: yesShipin gongye ke-ji
To enhance the expression level of recombinant alcohol dehydrogenase in E. coli BL21 (DE3), this study investigated the effects of induction temperature, induction time, and IPTG concentration on enzyme expression through one-way experiments ...
Hong TIAN   +6 more
doaj   +1 more source

Physiological and biochemical characterization of mango tree with paclobutrazol application via irrigation

open access: yesPesquisa Agropecuária Tropical, 2016
Paclobutrazol application in mango tree floral induction is followed by changes in the hormonal balance and carbohydrates production. This study aimed at evaluating the physiological and biochemical variables of mango tree (Palmer cultivar) by applying ...
Moisés Alves de Souza   +4 more
doaj   +1 more source

Whole-Cell Biocatalytic Production of Acetoin with an aldC-Overexpressing Lactococcus lactis Using Soybean as Substrate

open access: yesFoods, 2023
Douchi is a traditional Chinese fermented soybean product, in which acetoin is a key flavor substance. Here, the α-acetolactate decarboxylase gene aldC was cloned from Lactiplantibacillus (L.) plantarum and overexpressed in Lactococcus (L.) lactis NZ9000
Huajun Luo   +5 more
doaj   +1 more source

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

open access: yesFEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

Induction effect of chitosan on suppression of tomato early blight and its physiological mechanism

open access: yes浙江大学学报. 农业与生命科学版, 2003
Resistance to early blight of the tomato plants was induced by chitosan (1 mg/mL) at the fourth-leaf stage of seedlings. Chitosan treatment of tomato leaves before inoculation reduced the rates of diseased leaf and disease index.
LIAO Chun-yan, MA Guo-rui, HONG Wen-ying
doaj   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

open access: yesFEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy   +4 more
wiley   +1 more source

Xylose induces cellulase production in Thermoascus aurantiacus

open access: yesBiotechnology for Biofuels, 2017
Background Lignocellulosic biomass is an important resource for renewable production of biofuels and bioproducts. Enzymes that deconstruct this biomass are critical for the viability of biomass-based biofuel production processes.
Timo Schuerg   +16 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

open access: yesFEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa   +5 more
wiley   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

open access: yesFEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

DDIapp—A Web‐Based Application for Static Drug–Drug Interaction Assessment

open access: yesCPT: Pharmacometrics & Systems Pharmacology
In this work we describe the development of a web‐based application for static drug–drug interaction (DDI) risk assessment in accordance with the International Council for Harmonization (ICH) M12 guidance.
Hirotaka Watase   +5 more
doaj   +1 more source

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