Results 291 to 300 of about 2,217,916 (339)

Characterization of large transgene integrations in Chinese hamster ovary cells using a bioengineered mammalian transposase

Biotechnology Progress, EarlyView.
Abstract We present the first use of a bioengineered mammalian transposase system derived from Myotis lucifugus (bMLT) for integration of expression vectors into the CHO genome, focusing on GFP and trastuzumab production. Initially, CHO‐K1 cells are transfected with a GFP reporter and varying amounts of bMLT DNA or mRNA.
Nicolas Marx, Anna‐Maria Otte, Klaus Leitner, Rehmadanta Sitepu, Thomas Berger, Veronika Schäpertöns, Christian G. Huber, Quan Zhu, Sandeep Nema, Joseph J. Higgins, Nicole Borth   +10 more
wiley   +1 more source

Salicylic acid and jasmonic acid in plant immunity. [PDF]

Hortic Res
Zhang P, Jackson E, Li X, Zhang Y.
europepmc   +1 more source

Insights into the morphology‐productivity relationship of filamentous fungi through small‐scale cultivation and automated microscopy of Thermothelomyces thermophilus

Biotechnology Progress, EarlyView.
Abstract Filamentous fungi are a cornerstone in the biotechnological production of enzymes, proteins, and organic acids. However, challenges in understanding and controlling the relationship between morphology and productivity can limit their application.
Katja Rohr, Bertram Geinitz, Johannes Seiffarth, Aydin Anbarani, Sören Bernauer, Matthias Moch, Julia Tenhaef, Wolfgang Wiechert, Katharina Nöh, Marco Oldiges   +9 more
wiley   +1 more source

Fine Regulation of MicroRNAs in Gene Regulatory Networks and Pathophysiology. [PDF]

Int J Mol Sci
Seida M, Ogami K, Yoshino S, Suzuki HI.
europepmc   +1 more source

Enzyme Repression in the Regulation of Nicotinamide Adenine Dinucleotide Biosynthesis in Bacillus subtilis

, 1964
R.K. Gholson, Junko Kori
openalex   +1 more source

Membrane translocation and refolding mitochondrial proteins [PDF]

, 1990
Hartl, Franz-Ulrich, Neupert, Walter, Srere, Paul A.   +2 more
core  

A synthetic platform for developing recombinant adeno‐associated virus type 8 producer cell lines

Biotechnology Progress, EarlyView.
Abstract Recombinant adeno‐associated virus (rAAV) is one of the most widely used viral vectors for gene therapy. It is used in very high doses for the treatment of many diseases, making large‐scale production for clinical applications challenging. We have established a synthetic biology‐based platform to construct stable production cell lines, which ...
Yu‐Chieh Lin, Han‐Jung Kuo, Min Lu, Thomas Mahl, George Aslanidi, Wei‐Shou Hu   +5 more
wiley   +1 more source

Decoding microRNA arm switching: a key to evolutionary innovation and gene regulation. [PDF]

Cell Mol Life Sci
Pinhal D, Gonçalves LB, Campos VF, Patton JG.   +3 more
europepmc   +1 more source

5‐Aza combined with VPA reprograms human T lineage acute leukemia Jurkat cells into B‐cell‐like cells by epigenetic activation of PAX5

Biotechnology Progress, EarlyView.
Abstract Epigenetic regulation plays an important role in cell fate reprogramming. Here, we found that inhibitors of epigenetic modifiers, including VPA, TSA, and 5‐Aza‐2'‐deoxycytidine, can induce phenotypic transformation from Jurkat cells into B‐cell‐like cells. When Jurkat cells were treated with 5‐Aza combined with VPA, B cell and stem cell marker
Wenjin Xi, Guoxu Zheng, Xu Chen, Baile Zuo, Wei Wang, Yufang Li, Chunmei Zhang, Jie Chu, Xiuli Mu, Weihong Wen, Tao Wang, An‐Gang Yang   +11 more
wiley   +1 more source

The immune NIK1/RPL10/LIMYB signaling module regulates photosynthesis and translation under biotic and abiotic stresses. [PDF]

Nat Commun
Ferreira MA, Teixeira RM, Brustolini OJB, Saia TFF, Jean-Baptiste J, Ribeiro NGA, Breves SS, Sampaio FR, Santos EGD, Leon BA, Oliveira CC, Duarte CEM, Lima LL, Oliveira LL, Ramos HJO, Reis PAB, Fontes EPB.   +16 more
europepmc   +1 more source