Results 131 to 140 of about 594,398 (264)

Immobilisation of α-amylase on activated amidrazone acrylic fabric: a new approach for the enhancement of enzyme stability and reusability. [PDF]

Sci Rep, 2019
Al-Najada AR, Almulaiky YQ, Aldhahri M, El-Shishtawy RM, Mohamed SA, Baeshen M, Al-Farga A, Abdulaal WH, Al-Harbi SA.   +8 more
europepmc   +1 more source

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Immobilization of Thiol-Modified Horseradish Peroxidase on Gold Nanoparticles Enhances Enzyme Stability and Prevents Proteolytic Digestion. [PDF]

Langmuir
Breausche FE, Somerlot A, Walder J, Osei K, Okyem S, Driskell JD.   +5 more
europepmc   +1 more source

Hereditary tyrosinemia type I-associated mutations in fumarylacetoacetate hydrolase reduce the enzyme stability and increase its aggregation rate. [PDF]

J Biol Chem, 2019
Macias I, Laín A, Bernardo-Seisdedos G, Gil D, Gonzalez E, Falcon-Perez JM, Millet O.   +6 more
europepmc   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Solvent concentration at 50% protein unfolding may reform enzyme stability ranking and process window identification. [PDF]

Nat Commun
Sorgenfrei FA, Sloan JJ, Weissensteiner F, Zechner M, Mehner NA, Ellinghaus TL, Schachtschabel D, Seemayer S, Kroutil W.   +8 more
europepmc   +1 more source

Increasing Enzyme Stability and Activity through Hydrogen Bond-Enhanced Halogen Bonds. [PDF]

Biochemistry, 2018
Carlsson AC, Scholfield MR, Rowe RK, Ford MC, Alexander AT, Mehl RA, Ho PS.   +6 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Enzyme stability in polymer hydrogel-enzyme hybrid nanocarrier containing phosphorylcholine group. [PDF]

RSC Adv
Huang X, Li J, Araki Y, Wada T, Xu Y, Takai M.   +5 more
europepmc   +1 more source

Glucose-6-phosphate dehydrogenase enzyme stability in filter paper dried blood spots. [PDF]

Clin Biochem, 2017
Flores SR, Hall EM, De Jesús VR.
europepmc   +1 more source