Results 131 to 140 of about 989,780 (312)

Clinical performance of the urine‐based TERT promoter AbsoluteQ Digital PCR for non‐invasive detection of bladder cancer

Molecular Oncology, EarlyView.
A urine‐based digital PCR assay targeting two hotspot TERT promoter variants detected bladder cancer with high sensitivity and no false positives in this case–control cohort. The streamlined AbsoluteQ workflow outperformed Sanger sequencing and supports non‐invasive molecular testing for bladder cancer detection.
Anna Nykel, Izabela Kubiak, Lena Rutkowska, Żaneta Kasprzyk, Łukasz Kępczyński, Michał Bednarek, Dariusz Sobieraj, Jacek Wilkosz, Piotr Kania, Adam Jędrzejczyk, Bogdan Kałużewski, Agnieszka Gach, Tadeusz Kałużewski   +12 more
wiley   +1 more source

Enhancing Enzyme Stability for Biotechnological Applications

CHIMIA
Enzymes are emerging as a central element of green chemistry due to their high selectivity, biodegradability, and biocompatibility. However, their application in biotechnology is often limited by their poor stability under non-native conditions.
Ana I. Benítez-Mateos
doaj   +1 more source

Crowding Promotes the Switch from Hairpin to Pseudoknot Conformation in Human Telomerase RNA

, 2011
Formation of a pseudoknot in the conserved RNA core domain in the ribonucleoprotein human telomerase is required for function. In vitro experiments show that the pseudoknot (PK) is in equilibrium with an extended hairpin (HP) structure.
Antal M., Asakura S., Blasco M. A., Bloomfield V. A., Cao S., Cao S., Chen J. L., Cheung M. S., Comolli L. R., D. Thirumalai, Denesyuk N. A., Dhar A., Feng J., Gilley D., Hyeon C., Kilburn D., Kirkpatrick T. R., Leontis N. B., Lin J. C., McGuffee S. R., Montange R. K., Natalia A. Denesyuk, Pincus D. L., Shaw M. R., Theimer C. A., Theimer C. A., Thirumalai D., Walter H., Whitford P. C., Xia T., Zhou H.-X.   +30 more
core   +1 more source

Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining

Molecular Oncology, EarlyView.
IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis, Jack V. Mills, Wojciech Niedzwiedz, Valentine M. Macaulay   +3 more
wiley   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Biochemical characterization and gene structure analysis of the 24‐kDa glutathione transferase sigma from Taenia solium

FEBS Open Bio
Taenia solium can cause human taeniasis and/or cysticercosis. The latter can in some instances cause human neurocysticercosis which is considered a priority in disease‐control strategies and the prevention of mental health problems.
Ricardo Miranda‐Blancas, Oscar Rodríguez‐Lima, Ponciano García‐Gutiérrez, Roberto Flores‐López, Lucía Jiménez, Rafael A. Zubillaga, Enrique Rudiño‐Piñera, Abraham Landa   +7 more
doaj   +1 more source

Preparation, characterisation and application of naturally derived polar lipids through lipolysis : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Food Technology at Massey University, Palmerston North, New Zealand [PDF]

, 2016
Monoglycerides are lipid based emulsifiers extensively used for their broad technical function in the food industry. Commercial monoglycerides are generally manufactured through chemical synthesis; however, lipolysis of triglycerides by lipase enzyme ...
Sofian-Seng, Noor-Soffalina
core  

Tumor‐stromal crosstalk and macrophage enrichment are associated with chemotherapy response in bladder cancer

FEBS Open Bio, EarlyView.
Chemoresistance in bladder cancer: Macrophage recruitment associated with CXCL1, CXCL5 and CXCL8 expression is characteristic of Gemcitabine/Cisplatin (Gem/Cis) Non‐Responder tumors (right side) while Responder tumors did not show substantial tumor‐stromal crosstalk (left side). All biological icons are attributed to Bioicons: carcinoma, cancerous‐cell‐
Sophie Leypold, Janik Riese, Lancelot Seillier, Mark Kühnel, Julia Pannhausen, Charlotte O. J. Fröhlich, Christian Martin, Peter Boor, Matthias Saar, Danny D. Jonigk, Nadine T. Gaisa, Michael Rose   +11 more
wiley   +1 more source

Non-Aqueous Biocatalysis in Homogeneous Solvent Systems

Food Technology and Biotechnology, 2004
Enzymes are highly specific catalysts that typically function in aqueous solvents. However, many enzymes retain their catalytic activities at high concentrations in non- aqueous environments, including neat hydrophilic organic solvents.
Sebastián Torres, Guillermo R. Castro
doaj  

Thermoresponsive Poly(N,N-diethylacrylamide-co-glycidyl methacrylate) Copolymers and Its Catalytically Active α-Chymotrypsin Bioconjugate with Enhanced Enzyme Stability. [PDF]

Polymers (Basel), 2021
Kasza G, Stumphauser T, Bisztrán M, Szarka G, Hegedüs I, Nagy E, Iván B.   +6 more
europepmc   +1 more source