Results 201 to 210 of about 594,398 (264)

Engineering enzymes for stability

Current Opinion in Structural Biology, 1996
There have been many recent developments in elaborating the approaches for stabilizing enzymes by stabilizing the folding state, destabilizing the unfolded state and altering the kinetics of unfolding. However, these represent a series of rules of thumb rather than the reliable principles that would be expected of 'engineering'.
A, Shaw, R, Bott
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Enzyme stability in downstream processing part 1: Enzyme inactivation, stability and stabilization

Biotechnology Advances, 1992
In biotechnological recovery processes the instability of the product can lead to large losses in the sequence of recovery processes needed to purify the product. As the cost of the final active product is strongly dependent on the recovery yield, this will lead to an increase in product cost.
Weijers, S.R., van 't Riet, K.
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Stabilization of enzymes by their specific antibodies

Enzyme and Microbial Technology, 1991
In nature, increased stability of enzymes has often been found to be associated with noncovalent protein-protein interactions. Specific antibodies should be suitable for this purpose. To test this hypothesis, we used a number of model enzymes, complexed them with their specific antibodies, and exposed them and the free enzymes to low and high ...
E Y, Shami, M, Ramjeesingh, A, Rothstein, M, Zywulko   +3 more
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Structural stability of an enzyme biocatalyst

Biochemical Society Transactions, 2007
TK (transketolase) undergoes inactivation during biocatalytic processes due to oxidation, substrate and product inhibition, reactivity of aldehyde substrates, irreversible inactivation at low pH, and dissociation of cofactors. However, the contribution of protein denaturation to each of these mechanisms is not fully understood.
P A, Dalby, J P, Aucamp, R, George, R J, Martinez-Torres   +3 more
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The enzyme stability of dehydro-enkephalins

Peptides, 1982
Dehydro-enkephalins [delta Ala2]-, [delta Ala3]-, [delta Phe4]-, and [delta Leu5]enkephalins, were examined for their stability to enzymatic hydrolysis by carboxypeptidase Y [EC 3.4.16.1]. The successively liberated amino acids were determined quantitatively by amino acid analyses.
Y, Shimohigashi, H C, Chen, C H, Stammer
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Stabilization of Enzymes by Using Thermophiles

2023
Manufactured steroid compounds have many applications in the pharmaceutical industry. Due to the chemical complexity and chirality of steroids, there is an increasing demand for enzyme-based bioconversion processes to replace those based on chemical synthesis.
Ana-Luisa, Ribeiro, Mercedes, Sánchez, Sandra, Bosch, José, Berenguer, Aurelio, Hidalgo   +4 more
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Rational engineering of enzyme stability

Journal of Biotechnology, 2004
During the past 15 years there has been a continuous flow of reports describing proteins stabilized by the introduction of mutations. These reports span a period from pioneering rational design work on small enzymes such as T4 lysozyme and barnase to protein design, and directed evolution.
Eijsink, V.G., Bjork, A., Gaseidnes, S., Sirevag, R., Synstad, B., van der Burg, B., Vriend, G., Vriend, G.   +7 more
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Stabilization of food dispersions by enzymes

Food & Function, 2014
Food dispersions have become essential vehicles to carry and deliver functional ingredients such as bioactive compounds, flavors, antimicrobials, antioxidants, colors and vitamins. Most of these systems are thermodynamically unstable tending to break down over time.
Benjamin, Zeeb, Lutz, Fischer, Jochen, Weiss   +2 more
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Stabilization of Enzymes by Using Thermophiles

2017
Manufactured steroid compounds have many applications in the pharmaceutical industry. Due to the chemical complexity and chirality of steroids, there is an increasing demand for enzyme-based bioconversion processes to replace those based on chemical synthesis.
Ana Luisa, Ribeiro, Mercedes, Sánchez, Aurelio, Hidalgo, José, Berenguer   +3 more
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Enzyme stabilization: state of the art

Molecular and Cellular Biochemistry, 1991
'Enzyme stabilization' is one of the most important fields in basic and applied enzymology. In basic enzymology, it is of particular relevance to understand enzyme stabilization principles first elucidating how and why the enzymes lose their biological activity and then deriving structure-stability relationships existing in enzymatic molecules.
Gianfreda L, Scarfi' MR
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