Results 201 to 210 of about 560,659 (266)

Engineering enzymes for stability

Current Opinion in Structural Biology, 1996
There have been many recent developments in elaborating the approaches for stabilizing enzymes by stabilizing the folding state, destabilizing the unfolded state and altering the kinetics of unfolding. However, these represent a series of rules of thumb rather than the reliable principles that would be expected of 'engineering'.
A, Shaw, R, Bott
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The enzyme stability of dehydropeptides

Biochemical and Biophysical Research Communications, 1978
Abstract A pentapeptide, Z-Gly-Gly-Phe-Phe-Ala · OH (1b) and the corresponding unsaturated pentapeptide, Z-Gly-Gly-Phe-Δ Z Phe-Ala · OH (1a) , have been synthesized. The saturated compound (1b) was rapidly hydrolyzed by both chymotrypsin and thermolysin to the expected products, but the dehydropeptide was completely unhydrolyzed by ...
M L, English, C H, Stammer
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Enzyme stability in downstream processing part 1: Enzyme inactivation, stability and stabilization

Biotechnology Advances, 1992
In biotechnological recovery processes the instability of the product can lead to large losses in the sequence of recovery processes needed to purify the product. As the cost of the final active product is strongly dependent on the recovery yield, this will lead to an increase in product cost.
Weijers, S.R., van 't Riet, K.
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Stabilization of enzymes by their specific antibodies

Enzyme and Microbial Technology, 1991
In nature, increased stability of enzymes has often been found to be associated with noncovalent protein-protein interactions. Specific antibodies should be suitable for this purpose. To test this hypothesis, we used a number of model enzymes, complexed them with their specific antibodies, and exposed them and the free enzymes to low and high ...
E Y, Shami, M, Ramjeesingh, A, Rothstein, M, Zywulko   +3 more
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Rational engineering of enzyme stability

Journal of Biotechnology, 2004
During the past 15 years there has been a continuous flow of reports describing proteins stabilized by the introduction of mutations. These reports span a period from pioneering rational design work on small enzymes such as T4 lysozyme and barnase to protein design, and directed evolution.
Eijsink, V.G., Bjork, A., Gaseidnes, S., Sirevag, R., Synstad, B., van der Burg, B., Vriend, G., Vriend, G.   +7 more
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Structural stability of an enzyme biocatalyst

Biochemical Society Transactions, 2007
TK (transketolase) undergoes inactivation during biocatalytic processes due to oxidation, substrate and product inhibition, reactivity of aldehyde substrates, irreversible inactivation at low pH, and dissociation of cofactors. However, the contribution of protein denaturation to each of these mechanisms is not fully understood.
P A, Dalby, J P, Aucamp, R, George, R J, Martinez-Torres   +3 more
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Stabilization of Enzymes by Using Thermophiles

2023
Manufactured steroid compounds have many applications in the pharmaceutical industry. Due to the chemical complexity and chirality of steroids, there is an increasing demand for enzyme-based bioconversion processes to replace those based on chemical synthesis.
Ana-Luisa, Ribeiro, Mercedes, Sánchez, Sandra, Bosch, José, Berenguer, Aurelio, Hidalgo   +4 more
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The enzyme stability of dehydro-enkephalins

Peptides, 1982
Dehydro-enkephalins [delta Ala2]-, [delta Ala3]-, [delta Phe4]-, and [delta Leu5]enkephalins, were examined for their stability to enzymatic hydrolysis by carboxypeptidase Y [EC 3.4.16.1]. The successively liberated amino acids were determined quantitatively by amino acid analyses.
Y, Shimohigashi, H C, Chen, C H, Stammer
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Effect of Salts on Enzyme Stability

Annals of the New York Academy of Sciences, 1988
Pour tester la stabilite de trois enzymes en presence de solutions salines, les concentrations croissantes de chlorure de sodium, potassium ou lithium sont ajoutees a la preparation enzymatique. La demi-vie de l'enzyme est determinee. La sepctroscopie Raman permet d'etudier le degre d'organisation de l'environnement aqueux et d'expliquer en partie l ...
D, Combes, W N, Ye, A, Zwick, P, Monsan
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