Results 51 to 60 of about 978,452 (311)

Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification [PDF]

, 2016
A protocol for the production, purification, and use of enzyme packaged outer membrane vesicles (OMV) providing for enhanced enzyme stability for implementation across diverse applications is ...
Alves, Nathan J., Turner, Kendrick B., Walper, Scott A.   +2 more
core   +1 more source

Molecular bases of circadian magnesium rhythms across eukaryotes

FEBS Letters, EarlyView.
Circadian rhythms in intracellular [Mg2+] exist across eukaryotic kingdoms. Central roles for Mg2+ in metabolism suggest that Mg2+ rhythms could regulate daily cellular energy and metabolism. In this Perspective paper, we propose that ancestral prokaryotic transport proteins could be responsible for mediating Mg2+ rhythms and posit a feedback model ...
Helen K. Feord, Gerben van Ooijen
wiley   +1 more source

Pharmaceutically modified subtilisins withstand acidic conditions and effectively degrade gluten in vivo [PDF]

, 2019
Detoxification of gluten immunogenic epitopes is a promising strategy for the treatment of celiac disease. Our previous studies have shown that these epitopes can be degraded in vitro by subtilisin enzymes derived from Rothia mucilaginosa, a natural ...
Darwish, Ghassan, Helmerhorst, Eva J., Oppenheim, Frank G., Schuppan, Detlef, Wei, Guoxian   +4 more
core   +1 more source

Interplay between circadian and other transcription factors—Implications for cycling transcriptome reprogramming

FEBS Letters, EarlyView.
This perspective highlights emerging insights into how the circadian transcription factor CLOCK:BMAL1 regulates chromatin architecture, cooperates with other transcription factors, and coordinates enhancer dynamics. We propose an updated framework for how circadian transcription factors operate within dynamic and multifactorial chromatin landscapes ...
Xinyu Y. Nie, Jerome S. Menet
wiley   +1 more source

Stabilization of Enzymes in Silk Films [PDF]

Biomacromolecules, 2009
Material systems are needed that promote stabilization of entrained molecules, such as enzymes or therapeutic proteins, without destroying their activity. We demonstrate that the unique structure of silk fibroin protein, when assembled into the solid state, establishes an environment that is conducive to the stabilization of entrained proteins. Enzymes
Shenzhou, Lu, Xiaoqin, Wang, Qiang, Lu, Xiao, Hu, Neha, Uppal, Fiorenzo G, Omenetto, David L, Kaplan   +6 more
openaire   +2 more sources

Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing

FEBS Letters, EarlyView.
Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley   +1 more source

Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB

Molecules, 2014
It is well established that the performance of lipase B from Candida antarctica (CALB) as catalyst for esterification reactions may be improved by the use of ultrasound technology or by its immobilization on styrene-divinylbenzene beads (MCI-CALB).
Joana S. Alves, Cristina Garcia-Galan, Mirela F. Schein, Alexandre M. Silva, Oveimar Barbosa, Marco A. Z. Ayub, Roberto Fernandez-Lafuente, Rafael C. Rodrigues   +7 more
doaj   +1 more source

Dynamic Stability and Thermodynamic Characterization in an Enzymatic Reaction at the Single Molecule Level

, 2011
In this work we study, at the single molecular level, the thermodynamic and dynamic characteristics of an enzymatic reaction comprising a rate limiting step.
Chimal-Eguia, Chimal-Eguia, Esposito, Ge, Guzman-Vargas, Holmes, Huang, Huang, Huang, Huang, Huang, Huang, Kitano, Kitano, Lesne, Moisés Santillán, Nie, Nie, Páez-Hernández, Páez-Hernández, Qian, Santillán, Santillán, Santillán, Zeron   +24 more
core   +1 more source

PENGARUH SENYAWA KOFAKTOR DAN STABILITAS TERHADAP AKTIVITAS ENZIM ??-1,3- GLUKANASE DARI ISOLAT BAKTERI TERMOFIL Bacillus licheniformis HSA3-1a [PDF]

, 2012
Study on the effect of compound cofactor and stability to enzyme activity of ??-1, 3-glucanase has been done. This study used bacterial isolates B.
Dali, Seniwati, Gusti, Natsir, Hasnah
core   +2 more sources

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source