Results 51 to 60 of about 5,877,636 (396)

Approaching boiling point stability of an alcohol dehydrogenase through computationally-guided enzyme engineering

eLife, 2020
Enzyme instability is an important limitation for the investigation and application of enzymes. Therefore, methods to rapidly and effectively improve enzyme stability are highly appealing. In this study we applied a computational method (FRESCO) to guide
Friso S. Aalbers, Friso S. Aalbers, Maximilian J L J Fürst, Maximilian J L J Fürst, S. Rovida, M. Trajkovic, J. Castellanos, S. Bartsch, A. Vogel, A. Mattevi, M. Fraaije   +10 more
semanticscholar   +1 more source

Characterization of the novel ene reductase Ppo-Er1 from paenibacillus polymyxa [PDF]

, 2020
Ene reductases enable the asymmetric hydrogenation of activated alkenes allowing the manufacture of valuable chiral products. The enzymes complement existing metal- and organocatalytic approaches for the stereoselective reduction of activated C=C double ...
Aregger, David, Buller, Rebecca, Peters, Christin   +2 more
core   +1 more source

Bordonein-L, a new L-amino acid oxidase from Crotalus durissus terrificus snake venom: isolation, preliminary characterization and enzyme stability

Journal of Venomous Animals and Toxins including Tropical Diseases, 2015
BackgroundCrotalus durissus terrificus venom (CdtV) is one of the most studied snake venoms in Brazil. Despite presenting several well known proteins, its L-amino acid oxidase (LAAO) has not been studied previously.
Karla C F Bordon, G. Wiezel, H. Cabral, E. Arantes   +3 more
semanticscholar   +1 more source

Design of surface-active artificial enzyme particles to stabilize Pickering emulsions for high-performance biphasic biocatalysis [PDF]

, 2015
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Surface-active artificial enzymes (SAEs) are designed and constructed by a general and novel strategy.
Baciocchi, Binks, Bonomi, Chen, Chen, Crossley, Dewey, Diez-Castellnou, Gao, Jiang, Kapil, Kosif, Köhler, Li, Li, Lin, Liu, Liu, Mondloch, Nanda, Natalio, Neverov, Pengo, Pera-Titus, Silverman, Smith, Song, Sun, Tonga, Vernekar, Wang, Wang, Wang, Wiese, Wu, Yang, Zapata, Zaramella, Zhang, Zhou   +39 more
core   +1 more source

Enhanced oral absorption of insulin: hydrophobic ion pairing and a self-microemulsifying drug delivery system using a D-optimal mixture design

Drug Delivery, 2022
The lipophilicity of a peptide drug can be considerably increased by hydrophobic ion pairing with amphiphilic counterions for successful incorporation into lipid-based formulations.
Yoon Tae Goo, Sangkil Lee, Ji Yeh Choi, Min Song Kim, Gi Hyeong Sin, Sun Ho Hong, Chang Hyun Kim, Seh Hyon Song, Young Wook Choi   +8 more
doaj   +1 more source

Solvent and thermal stability, and pH kinetics, of proline-specific dipeptidyl peptidase IV-like enzyme from bovine serum [PDF]

, 2007
Proline-specific dipeptidyl peptidase-like (DPP IV; EC 3.4.14.5) activity in bovine serum has attracted little attention despite its ready availability and the paucity of useful proline-cleaving enzymes.
Abbott, Barry, Brendan F. O’Connor, Buckley, Bušek, Chruscinska, Ciarán Ó’Fágáin, De Meester, Deborah M. Ruth, Demuth, Dixon, Duke-Cohan, Dunn, Durinx, Edens, Engel, Fersht, FitzGerald, Gonzalez, Gorren, Grasso, Jagath, Khmelnitsky, Kikkawa, Lambeir, Lee, Maes, Mentlein, Mittal, Ogino, Ohkubo, Ohnishi, O’Cuinn, Poltorak, Poltorak, Rasmussen, Sears, Sentandreu, Sheehan, Smith, Séamus J. Buckley, Trubetskoy, Yaron, Yoshimoto   +43 more
core   +1 more source

Immobilization of Lipase from Thermomyces Lanuginosus and Its Glycerolysis Ability in Diacylglycerol Preparation

Molecules
In the glycerolysis process for diacylglycerol (DAG) preparation, free lipases suffer from poor stability and the inability to be reused. To address this, a cost-effective immobilized lipase preparation was developed by cross-linking macroporous resin ...
Rui Xie, Yee-Ying Lee, Pengkai Xie, Chin-Ping Tan, Yong Wang, Zhen Zhang   +5 more
doaj   +1 more source

Natural Variants of the KPC-2 Carbapenemase have Evolved Increased Catalytic Efficiency for Ceftazidime Hydrolysis at the Cost of Enzyme Stability

PLoS Pathogens, 2015
The spread of β-lactamases that hydrolyze penicillins, cephalosporins and carbapenems among Gram-negative bacteria has limited options for treating bacterial infections.
Shrenik C. Mehta, Kacie Rice, T. Palzkill   +2 more
semanticscholar   +1 more source

The Effect of Calcium Alginate Entrapment on the Stability of Novel Lipases from P. Reinekei and P. brenneri

Trends in Peptide and Protein Sciences, 2019
The high cost of soluble enzymes can limit their use for commercial and industrial purposes. Immobilization can enhance enzyme reusability, thereby reducing product isolation costs and overcoming this economic barrier.
Priyanka Priyanka, Gemma K. Kinsella, Gary T. Henehan, Barry J. Ryan   +3 more
doaj   +1 more source

Immobilization of α-galactosidase from Clitocybe squamulose and its Hydrolysis of Oigosaccharides in Soybean Milk

Liang you shipin ke-ji, 2022
Sodium alginate embedding method and chitosan crosslinking method were used to immobilize α -galactosidase from Clitocybe squamulosa. The optimum pH, pH stability, optimum temperature, temperature stability, storage time of immobilized enzyme and free ...
ZUO Ning-ke, CHANG Ming-chang, MENG Jun-long, WANG Zhao-yu, WU Bin, XU Li-jing   +5 more
doaj   +1 more source