Results 71 to 80 of about 989,780 (312)
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
Considerations when Measuring Biocatalyst Performance
Molecules, 2019 As biocatalysis matures, it becomes increasingly important to establish methods with which to measure biocatalyst performance. Such measurements are important to assess immobilization strategies, different operating modes, and reactor configurations ...
Mafalda Dias Gomes, John M. Woodley
doaj +1 more source
Immobilization of Isocitrate Dehydrogenase on Mesoporous Silica Foam for Carbon Dioxide Capture [PDF]
E3S Web of Conferences, 2019 Carbon capture can be realized effectively through isocitrate dehydrogenase reaction and the reaction rate was strongly affected by the environmental parameters such as pH and temperature.
Xia Shunxiang +2 more
doaj +1 more source
A novel enzymatic approach to nanocrystalline cellulose preparation [PDF]
, 2018 In this work, conditions for an enzymatic pretreatment prior to NCC isolation from cotton linter were assessed. Different cellulase doses and reaction times were studied within an experimental design and NCC were obtained. At optimal enzymatic conditions
Beltramino, Facundo +3 more
core +2 more sources
Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system
FEBS Letters, EarlyView.Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley +1 more source
, 2015 We analyse the effect of intrinsic fluctuations on the properties of bistable stochastic systems with time scale separation operating under1 quasi-steady state conditions.
Fabian Spill +11 more
core +1 more source
Fully Integrated Biochip Platforms for Advanced Healthcare [PDF]
, 2012 Recent advances in microelectronics and biosensors are enabling developments of innovative biochips for advanced healthcare by providing fully integrated platforms for continuous monitoring of a large set of human disease biomarkers.
Baj-Rossi, Camilla +11 more
core +5 more sources
FEBS Letters, EarlyView.This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel +13 more
wiley +1 more source
The equilibrium model for the effect of temperature on enzymes: Insights and implications [PDF]
, 2008 A new, experimentally-validated “Equilibrium Model” describes the effect of temperature on enzymes, and provides a new mechanism for the reversible loss of enzyme activity with temperature.
Daniel, Roy M. +4 more
core +1 more source
Peptide synthesis by recombinant Fasciola hepatica cathepsin L1 [PDF]
, 2006 Synthesis of the tripeptide Z-Phe-Arg-SerNH2 has been accomplished by a recombinant cysteine protease, cathepsin L1 from liver fluke (Fasciola hepatica), using Z-Phe-Arg-OMe as acyl acceptor and SerNH2 as nucleophile in 0.1 M ammonium acetate pH 9.0–12.5%
Ciarán Ó'Fágáin +16 more
core +1 more source