Journal of Bioinformatics and GenomicsВ работе исследовано влияние различных методов предварительного замораживания на эффективность последующей лиофилизации коллагеназы типа I и сохранение ее ферментативной активности.
Полякова А.О. +1 more
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Mutations of Rad6 E2 ubiquitin-conjugating enzymes at alanine-126 in helix-3 affect ubiquitination activity and decrease enzyme stability. [PDF]
J Biol Chem, 2022 Shukla PK +9 more
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Molecular Oncology, EarlyView.The novel styrylquinazolinone‐based molecule W1B effectively suppresses glioblastoma by inhibiting IGF1R and EGFR. In high‐glucose microenvironments driving tumor resistance, W1B acts synergistically with the EGFR inhibitor dacomitinib. This combination safely blocks compensatory survival signaling in zebrafish xenograft models. Showcasing promising in
Patryk Rurka +9 more
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Shipin yu jixieObjectiveTo purify and study the enzymatic properties of acid protease from the Trichoderma reesei strain, and to screen the stabilizer formula.MethodsThe acid protease is purified by ammonium sulfate precipitation, dialysis, and anion exchange column ...
WU Yao +6 more
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Evaluation of pancreatin stability through enzyme activity determination
Acta Pharmaceutica, 2016 Pancreatin is a biotechnological product containing an enzyme complex, obtained from porcine pancreas, that is employed in treating pancreatic diseases.
Terra Gleysson De Paula +3 more
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Aqueous ionic liquids redistribute local enzyme stability via long-range perturbation pathways. [PDF]
Comput Struct Biotechnol J, 2021 El Harrar T +5 more
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Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization
Molecular Oncology, EarlyView.In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu +13 more
wiley +1 more source
Non-thermal hydrodynamic cavitation processing of tomato juice for physicochemical, bioactive, and enzyme stability: Effect of process conditions, kinetics, and shelf-life extension. [PDF]
Curr Res Food Sci, 2022 Vigneshwaran G, More PR, Arya SS.
europepmc +1 more source
The C-terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity. [PDF]
FEBS Open BioArginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Yadav VK +4 more
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Loss of proton‐sensing TDAG8 increases tumor progression in mouse models of colon cancer
Molecular Oncology, EarlyView.Loss of the pH‐sensing receptor TDAG8 accelerates colorectal cancer progression in mice. Animals lacking TDAG8 expression had increased tumor growth, DNA damage, and recruitment of tumor‐associated immune cells, including macrophages, neutrophils, and monocytes.
Ermanno Malagola +11 more
wiley +1 more source