Results 311 to 320 of about 2,250,147 (369)

Enzyme immobilization in porous solid supports—penetration of immobilized enzyme

Biotechnology and Bioengineering, 1984
AbstractThe process of enzyme immobilization under the diffusion‐controlled regime (i.e., fast attachment of enzyme compared to its diffusion) is modeled and theoretically solved in this article. Simple and compact solutions for the penetration depth of immobilized enzyme and the bulk enzyme concentration versus time are presented.
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Nanoporous Gold for Enzyme Immobilization

2010
Nanoporous gold (NPG) is a material of emerging interest for immobilization of biomolecules, especially enzymes. The material provides a high surface area form of gold that is suitable for physisorption or for covalent modification by self-assembled monolayers.
Keith J. Stine, Kenise Jefferson, Olga V. Shulga   +2 more
openaire   +4 more sources

Thermal Stabilization of Enzymes Immobilized within Carbon Paste Electrodes.

Analytical Chemistry, 1997
In this note we report on the remarkable thermal stabilization of enzymes immobilized in carbon paste electrodes. Amperometric biosensors are shown for the first time to withstand a prolonged high-temperature (>50 °C) stress.
J. Wang, J. Liu, G. Cepra
semanticscholar   +1 more source

[53] Reactivation of immobilized enzymes

1987
Publisher Summary This chapter discusses a common feature of the irreversible inactivation processes that the denatured protein is unable to return to the native state N––that is, to reactivate spontaneously after the removal of the denaturant. The principal mechanism for the inactivation of immobilized enzymes is denaturation due to irreversible ...
Vadim V. Mozhaev, Karel Martinek, I.V. Berezin   +2 more
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Immobilization of enzymes on PTFE surfaces

Biotechnology and Bioengineering, 2001
Membranes and powders prepared from PTFE (polytetrafluorethylene) were investigated for their potential use as multifunctional supports for enzymes. The obtained bioactive materials are valuable for the construction of biosensors and enzyme reactors. To allow covalent coupling of enzymes to PTFE, the surface of the material was treated with elementary ...
Michael Keusgen, Peter Milka, Janina Glodek, Ingo Krest   +3 more
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Enhanced activity of enzymes immobilized in thermoresponsive core-shell microgels.

Journal of Physical Chemistry B, 2009
We present a quantitative study of the catalytic activity of beta-d-glucosidase from almonds adsorbed on thermosensitive microgels. The core-shell particles used as a carrier system consist of a solid polystyrene core onto which a poly(N ...
Nicole Welsch, A. Wittemann, M. Ballauff
semanticscholar   +1 more source

[49] Immobilized-enzyme reactors

1976
Publisher Summary The development the methods for the immobilization has produced novel forms of biochemical catalyst, which can be used in reactors to carry out conversions of industrial interest. Many different types of reactor have been employed with immobilized enzymes.
P Dunnill, M D Lilly
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Nanodevices for the immobilization of therapeutic enzymes

Critical Reviews in Biotechnology, 2015
Therapeutic enzymes are one of the most promising applications of this century in the field of pharmaceutics. Biocatalyst properties can be improved by enzyme immobilization on nano-objects, thereby increasing stability and reusability and also enhancing the targeting to specific tissues and cells.
Bosio, Valeria Elizabeth, Islan, German Abel, Martinez, Nelida Yanina, Durán, Nelson, Castro, Guillermo Raul   +4 more
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Protein analysis using enzymes immobilized to paramagnetic beads.

Analytical Biochemistry, 1999
A new method combining protein chemistry with enzymes immobilized to paramagnetic beads is presented. The immobilized enzymes can substitute for regular enzymes in a number of protein chemistry protocols, resulting in faster reaction times, less sample ...
T. Krogh, T. Berg, P. Højrup
semanticscholar   +1 more source

Enzymes Immobilized on Lumen [PDF]

, 2015
This particular kind of immobilization is generally referred to enzymes immobilized into the membrane lumen surface of tubular membranes for the production of biocatalytic membrane and biocatalytic membrane reactors. A scheme of a cross section of a biocatalytic asymmetric hollow fiber membrane is reported in Fig.
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