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Nanotechnology in Enzyme Immobilization: An Overview on Enzyme Immobilization with Nanoparticle Matrix

Current Nanoscience, 2019
Engineering of biocatalysts with the help of immobilization techniques is a worthy approach for the advancement of enzyme function and stability and is finer to the other chemical as well as biological methods. These biocatalysts encapsulation methods actually use very gentle method conditions that hardly affect biocatalysts internal specific ...
Abha Mishra   +3 more
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Enzyme Immobilization by Adsorption

2003
Much has already been written on the subject of biocatalyst (cell/enzyme) immobilization, and it is not the function of a text such as this to go into all the various aspects of the field. For general reviews on the area, the reader is referred to refs. 1-3.
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Biocatalysis with enzymes immobilized on mesoporous hosts: the status quo and future trends

, 2010
Biocatalysis opens the door to green and sustainable processes in synthetic chemistry, in the production of natural products, and in waste management.
M. Hartmann, Dirk Jung
semanticscholar   +1 more source

Enzyme Immobilization by Entrapment

2003
Entrapment methods of immobilization are mostly used in cell immobilization procedures, but have found some application with enzymes, particularly when the enzyme is essentially a dead cell or crude homogenate(1). In theory the entrapped enzyme is not attached to the polymer; its free diffusion is merely restrained. In practice, however, some or all of
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Remarkable thermostability of bioelectrodes based on enzymes immobilized within hydrophobic semi‐solid matrices

Biotechnology and applied biochemistry, 1999
An enhanced resistance to thermal denaturation was investigated for enzymes immobilized within hydrophobic semi‐solid matrices compared with both free enzymes and polymer‐entrapped enzymes. The bioelectrodes based on the immobilization of glucose oxidase,
Jie Liu, Joseph Wang
semanticscholar   +1 more source

The Immobilization of Enzymes on Inorganic Supports

Biochemical Society Transactions, 1974
In rats exposed to either 300 or 500 pulses/min no significant light-microscopic or electron-microscopic changes were observed. The activities of alkaline phosphatase, 5'-nucleotidase, isocitrate dehydrogenase, a-glycerophosphate dehydrogenase, lactate dehydrogenase, NADH-diaphorase and monoamine oxidase did not change significantly in brain or liver ...
THORNTON, DAVID   +3 more
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Redox enzymes immobilized on electrodes with solution cosubstrates. General procedure for simulation of time-resolved catalytic responses.

Analytical Chemistry, 2006
In view of the existing and potential applications of electrochemical enzymatic catalysis with redox enzymes immobilized on the electrode surface in biosensors, a numerical calculation procedure for simulating their cyclic voltammetric responses is ...
C. Andrieux   +3 more
semanticscholar   +1 more source

Activity of enzymes immobilized in colloidal spherical polyelectrolyte brushes.

Biomacromolecules, 2005
We investigate the enzymatic activity of glucoamylase and beta-glucosidase adsorbed on a novel type of colloidal particles. The particles used consist of a poly(styrene) core onto which long chains of poly(acrylic acid) or of poly(styrene sulfonic acid ...
Björn Haupt   +3 more
semanticscholar   +1 more source

Kinetic Measurements for Enzyme Immobilization

2010
Enzyme kinetics is the study of the chemical reactions that are catalyzed by enzymes, with a focus on their reaction rates. The study of an enzyme's kinetics considers the various stages of activity, reveals the catalytic mechanism of the enzyme, correlates its value to assay conditions, and describes how a drug or a poison might inhibit the enzyme ...
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[4] Enzymes immobilized to cellulose

1976
Publisher Summary This chapter discusses the immobilization of a variety of enzymes to cellulose. The availability of modified celluloses, originally for use as ion exchangers, has permitted a wide range of methods to be developed for covalent binding of enzymes.
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