Results 51 to 60 of about 161,575 (283)

Reductive Biotransformation of Ethyl Acetoacetate: A Comparative Studies using Free and Immobilized Whole Yeast Cells [PDF]

, 2008
Bioreduction of ethyl acetoacetate with free and immobilized yeast whole cell was achieved by using water and sucrose combination. After detachment from immobilized beads under basic condition, the corresponding ethyl(S)-(+)-3-hydroxybutanoate was ...
Amit R. Kumbhar, Madhav B. Pande, Sachin S. Laddha, Vaibhav V. Kulkarni   +3 more
core   +1 more source

Design of surface-active artificial enzyme particles to stabilize Pickering emulsions for high-performance biphasic biocatalysis [PDF]

, 2015
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Surface-active artificial enzymes (SAEs) are designed and constructed by a general and novel strategy.
Baciocchi, Binks, Bonomi, Chen, Chen, Crossley, Dewey, Diez-Castellnou, Gao, Jiang, Kapil, Kosif, Köhler, Li, Li, Lin, Liu, Liu, Mondloch, Nanda, Natalio, Neverov, Pengo, Pera-Titus, Silverman, Smith, Song, Sun, Tonga, Vernekar, Wang, Wang, Wang, Wiese, Wu, Yang, Zapata, Zaramella, Zhang, Zhou   +39 more
core   +1 more source

Peroxidasin enables melanoma immune escape by inhibiting natural killer cell cytotoxicity

Molecular Oncology, EarlyView.
Peroxidasin (PXDN) is secreted by melanoma cells and binds the NK cell receptor NKG2D, thereby suppressing NK cell activation and cytotoxicity. PXDN depletion restores NKG2D signaling and enables effective NK cell–mediated melanoma killing. These findings identify PXDN as a previously unrecognized immune evasion factor and a potential target to improve
Hsu‐Min Sung, David Bickel, Lena C. M. Krause, Daria Ezeriņa, Christian Ickes, Julian Wojtachnia, Christine S. Gibhardt, Magdalena Shumanska, Khadija Wahni, Andrea Paluschkiwitz, Julia Malo Pueyo, Ekaterina Baranova, Wim Vranken, Hedwig Stanisz, Ioana Stejerean‐Todoran, Michael P. Schön, Joris Messens, Ivan Bogeski   +17 more
wiley   +1 more source

Semi-Continuous Flow Biocatalysis with Affinity Co-Immobilized Ketoreductase and Glucose Dehydrogenase

Molecules, 2020
The co-immobilization of ketoreductase (KRED) and glucose dehydrogenase (GDH) on highly cross-linked agarose (sepharose) was studied. Immobilization of these two enzymes was performed via affinity interaction between His-tagged enzymes (six histidine ...
Michal Plž, Tatiana Petrovičová, Martin Rebroš   +2 more
doaj   +1 more source

Dual-Enzyme Cascade Composed of Chitosan Coated FeS₂ Nanozyme and Glucose Oxidase for Sensitive Glucose Detection

Molecules, 2023
Immobilizing enzymes with nanozymes to catalyze cascade reactions overcomes many of the shortcomings of biological enzymes in industrial manufacturing.
Bowen Shen, Molan Qing, Liying Zhu, Yuxian Wang, Ling Jiang   +4 more
doaj   +1 more source

A novel mechanism of RNase L inhibition: Theiler\u27s virus L* protein prevents 2-5A from binding to RNase L [PDF]

, 2018
The OAS/RNase L pathway is one of the best-characterized effector pathways of the IFN antiviral response. It inhibits the replication of many viruses and ultimately promotes apoptosis of infected cells, contributing to the control of virus spread ...
Drappier, Melissa, Elliot, Ruth, Jha, Babal Kant, Michiels, Thomas, Silverman, Robert H, Stone, Sasha, Vertommen, Didier, Weiss, Susan R, Zhang, Rong   +8 more
core   +4 more sources

Rethinking plastic waste: innovations in enzymatic breakdown of oil‐based polyesters and bioplastics

FEBS Open Bio, EarlyView.
Plastic pollution remains a critical environmental challenge, and current mechanical and chemical recycling methods are insufficient to achieve a fully circular economy. This review highlights recent breakthroughs in the enzymatic depolymerization of both oil‐derived polyesters and bioplastics, including high‐throughput protein engineering, de novo ...
Elena Rosini, Nicolò Antonelli, Gianluca Molla   +2 more
wiley   +1 more source

Biofabrication of magnetic nanoparticles and their use as carriers for pectinase and xylanase

OpenNano, 2022
In this study, superparamagnetic iron oxide nanoparticles (MNPs) were synthesized via exposure of fungal cell filtrate from Aspergillus flavus to aqueous iron ions.
Shady S. Hassan, Brendan Duffy, Gwilym A. Williams, Amit K. Jaiswal   +3 more
doaj   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Facile immobilization of pyridoxal 5′-phosphate using p-diazobenzoyl-derivatized Sepharose 4B

Results in Chemistry, 2023
Pyridoxal 5′-phosphate (PLP) is a ubiquitous and versatile cofactor utilized by numerous enzymes involved in amino acid biosynthetic pathways. Immobilized PLP is a valuable tool to isolate unknown PLP-dependent enzymes in nature or to perform in vitro ...
Kun-Hwa Lee, Mark D. Distefano, Burckhard Seelig   +2 more
doaj   +1 more source