Results 341 to 350 of about 554,474 (398)

Expression and purification of E140 protein antigen fragments of Plasmodium vivax and Plasmodium berghei for serological assays

FEBS Open Bio, EarlyView.
We provide a step‐by‐step guide for producing E140 antigen fragments from Plasmodium berghei (Pb1) and Plasmodium vivax (Pv1). Pb1/Pv1 are expressed in E. coli, solubilized by freeze–thawing, refolded by slow dilution, purified by affinity chromatography (IMAC), then concentrated and subjected to quality control.
Rodolfo Ferreira Marques, Edit Ábrahám, Hiromi Muramatsu, Daniel Youssef Bargieri, Norbert Pardi, Zoltán Lipinszki   +5 more
wiley   +1 more source

Human responses to the DNA prime/chimpanzee adenovirus (ChAd63) boost vaccine identify CSP, AMA1 and TRAP MHC Class I-restricted epitopes. [PDF]

PLoS One
Ganeshan H, Huang J, Belmonte M, Belmonte A, Inoue S, Velasco R, Maiolatesi S, Limbach K, Patterson N, Sklar MJ, Soisson L, Epstein JE, Edgel KA, Peters B, Hollingdale MR, Villasante E, Duplessis CA, Sedegah M.   +17 more
europepmc   +1 more source

PIPLOM: prediction of exogenous peptide loading on major histocompatibility complex class I molecules. [PDF]

Bioinform Adv
Schmidt F, Wu K, Gerber L, Chioh Wen Jing F, Pedrosa D, Wong Choon Lim G, Wirawan M, Eng C, Fink K, MacLeod DT, Fehlings M, Wilm A.   +11 more
europepmc   +1 more source

CD44v, S1PR1, HER3, MET and cancer‐associated amino acid transporters are promising targets for the pancreatic cancers characterized using mAb

FEBS Open Bio, EarlyView.
Novel mAbs could detect cancer‐associated membrane proteins on various type of pancreatic ductal adenocarcinomas (PDAC) by flow cytometry with viable PDAC cells and by immunohistochemistry with PDAC tissues. Results of protein expression were substantiated by mRNA expression by The Cancer Genome Atlas.
Takashi Nakano, Kouki Okita, Shogo Okazaki, Soshi Yoshimoto, Sachiko Masuko, Hideki Yagi, Kazunori Kato, Yoshihisa Tomioka, Kenichi Imai, Yoichi Hamada, Kazue Masuko, Kayoko Shimada‐Takaura, Noriaki Nagai, Hideyuki Saya, Tomio Arai, Toshiyuki Ishiwata, Takashi Masuko   +16 more
wiley   +1 more source

Purification and characterization of a thermophilic NAD+‐dependent lactate dehydrogenase from Moorella thermoacetica

FEBS Open Bio, EarlyView.
The thermophilic acetogenic model organism Moorella thermoacetica can utilize lactate as substrate. The formation of lactate by reduction of pyruvate is energetically favorable, but the reverse reaction, the oxidation of lactate to pyruvate with NAD+ as electron acceptor is challenging.
Florian P. Rosenbaum, Volker Müller
wiley   +1 more source

Design of a multi-epitope vaccine candidate against Vibrio cholerae. [PDF]

Sci Rep
Koupaei FN, Kiumarsy A, Feizi MM, Vajdi Z, Edalat Pishkar S, Alem M, Roohparvar Basmenj E.   +6 more
europepmc   +1 more source

Developing T Cell Epitope-Based Vaccines Against Infection: Challenging but Worthwhile. [PDF]

Vaccines (Basel)
Tang X, Zhang W, Zhang Z.
europepmc   +1 more source

DECODE enables high-throughput mapping of antibody epitopes at single amino acid resolution. [PDF]

PLoS Biol
Matsumoto K, Harada SY, Yoshida SY, Narumi R, Mitani TT, Yada S, Sato A, Morii E, Shimizu Y, Ueda HR.   +9 more
europepmc   +1 more source