Results 221 to 230 of about 48,164 (260)
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Mass spectrometric epitope mapping
Mass Spectrometry Reviews, 2016Mass spectrometric epitope mapping has become a versatile method to precisely determine a soluble antigen's partial structure that directly interacts with an antibody in solution. Typical lengths of investigated antigens have increased up to several 100 amino acids while experimentally determined epitope peptides have decreased in length to on average ...
Opuni, Kwabena F.M. +5 more
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Mapping conformational epitopes by NMR spectroscopy
Current Opinion in Virology, 2021Antibodies recognize their target with high affinity and specificity. This is important for pathogen neutralization, which plays a crucial role in defense against disease. Antibodies are powerful tools in the development of new therapeutics, such as vaccines, to fight diseases such as viral infections and even cancer.
Ana P, Valente +1 more
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Epitope Mapping by Phage Display
2017Among the molecules of the immune system, antibodies, particularly monoclonal antibodies (mAbs), have been shown to be interesting for many biological applications. Due to their ability to recognize only a unique part of their target, mAbs are usually very specific.
Gustavo Marçal Schmidt Garcia, Moreira +2 more
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An Overview of Epitopes and Methods for Antibody Epitope Mapping
Antibodies are protein molecules indispensable for many therapeutic, diagnostic, and research purposes due to their exquisite ability to selectively recognize and bind a given antigen. The particular area of the antigen recognized by the antibody is called the epitope, and mapping of such epitopes can provide important mechanistic insights and indicateJohan, Nilvebrant +3 more
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2001
Abstract Epitope Mapping covers all the major methods for the identification and definition of epitopes. The Pepscan assay is used to define B cell epitopes and makes use of synthetic peptides but can only be used if the amino acid sequence is known. It can be adapted for the delineation of both helper T cells and cytotoxic T cells.
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Abstract Epitope Mapping covers all the major methods for the identification and definition of epitopes. The Pepscan assay is used to define B cell epitopes and makes use of synthetic peptides but can only be used if the amino acid sequence is known. It can be adapted for the delineation of both helper T cells and cytotoxic T cells.
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Mapping the autoimmunizing epitopes on acetylcholine receptors
Immunology Today, 1987In the autoimmune disease myasthenia gravis (MG), patients experience muscle weakness and fatiguability owing to loss of functional acetylcholine receptors (AChR) at the neuromuscular junction. The loss may be caused by modulation of the receptors by anti-AChR autoantibody and/or by complement-mediated focal lysis at the post-synaptic membrane.
G, Harcourt, A, Jermy
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2009
Despite its unequivocal benefits to humankind, vaccine design and development has always been an inherently laborious and a largely empirical process; the unfortunate lack of a rational basis for vaccinology has hitherto stymied the commercial exploitation of vaccine discovery and also the deployment of vaccination as the principal, global instrument ...
Matthew N. Davies, Darren R. Flower
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Despite its unequivocal benefits to humankind, vaccine design and development has always been an inherently laborious and a largely empirical process; the unfortunate lack of a rational basis for vaccinology has hitherto stymied the commercial exploitation of vaccine discovery and also the deployment of vaccination as the principal, global instrument ...
Matthew N. Davies, Darren R. Flower
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2003
Monoclonal and polyclonal antibodies are important tools in molecular and cell biology, allowing protein molecules to be purified (1), identified in immunoblots of gels (2) and sections of biological material (3), and probed for functionally active regions.
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Monoclonal and polyclonal antibodies are important tools in molecular and cell biology, allowing protein molecules to be purified (1), identified in immunoblots of gels (2) and sections of biological material (3), and probed for functionally active regions.
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Epitope Mapping of Flavivirus Glycoproteins
1986Publisher Summary The vast majority of monoclonal antibodies to flaviviruses produced so far are specific for the E glycoprotein; therefore, this chapter deals with the characteristics of epitopes on this immunologically dominant protein. In addition, recent studies with NV3-specific monoclonal antibodies provide evidence that this nonstructural ...
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An introduction to epitope mapping
2001Abstract Antigens are highly diverse, varying in size as well as the composition of their primary sequence. In the case of proteins, post-translational processing with the addition of moieties, such as carbohydrates during glycosylation, may alter secondary structure. The relative location of epitopes on the surface of an antigen demands
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