Results 221 to 230 of about 4,711 (242)
Some of the next articles are maybe not open access.
Epitranscriptomic technologies and analyses
Science China Life Sciences, 2020RNA can interact with RNA-binding proteins (RBPs), mRNA, or other non-coding RNAs (ncRNAs) to form complex regulatory networks. High-throughput CLIP-seq, degradome-seq, and RNA-RNA interactome sequencing methods represent powerful approaches to identify biologically relevant ncRNA-target and protein-ncRNA interactions.
Xiaoyu, Li +8 more
openaire +2 more sources
Epitranscriptomics in fibroblasts and fibrosis
American Journal of Physiology-Cell Physiology, 2022Fibroblasts play an important role in the pathogenic mechanisms of several socially significant diseases, including pulmonary and cardiovascular fibrosis, liver cirrhosis, systemic sclerosis, progressive kidney disease. The alterations of the epitranscriptome, including more than 170 distinct posttranscriptional RNA modifications or editing events ...
Mirolyuba Ilieva, Shizuka Uchida
openaire +3 more sources
N6-methyladenosine–encoded epitranscriptomics
Nature Structural & Molecular Biology, 2016N6-methyladenosine (m6A) is the most abundant internal modification in eukaryotic mRNA. Recent discoveries of the locations, functions and mechanisms of m6A have shed light on a new layer of gene regulation at the RNA level, giving rise to the field of m6A epitranscriptomics. In this Perspective, we provide an update on the various effects of mammalian
Nian, Liu, Tao, Pan
openaire +2 more sources
Bioinformatic tools for epitranscriptomics
American Journal of Physiology-Cell Physiology, 2023The epitranscriptome, defined as RNA modifications that do not involve alterations in the nucleotide sequence, is a popular topic in the genomic sciences. Because we need massive computational techniques to identify epitranscriptomes within individual transcripts, many tools have been developed to infer epitranscriptomic sites as well as to process ...
openaire +2 more sources
Resolving m6A epitranscriptome with stoichiometry
Trends in Genetics, 2022A recent study by Hu et al. describes N6-methyladenosine (m6A)-selective allyl chemical labeling and sequencing (m6A-SAC-seq), which allows for quantitative, stoichiometric, and positional analyses of m6A at single-nucleotide resolution across the whole transcriptome level.
Ki-Jun Yoon, Yoon Ki Kim
openaire +2 more sources
Detection techniques for epitranscriptomic marks
American Journal of Physiology-Cell Physiology, 2022Similar to epigenetic DNA modification, RNA can be methylated and altered for stability and processing. RNA modifications, namely, epitranscriptomes, involve the following three functions: writing, erasing, and reading of marks. Methods for measurement and position detection are useful for the assessment of cellular function and human disease ...
Ken Ofusa +2 more
openaire +2 more sources
Epitranscriptomic Mass Spectrometry
Every chemical group that is added to any one of the canonical ribonucleotides in a transcript would create a specific RNA modification. Currently, 170+ RNA modifications have been identified. A specific epitranscriptome refers to all the RNA modifications in a given biological system and is considered to play an important role in the regulations of ...Hongzhou, Wang +3 more
openaire +2 more sources
Epitranscriptomics and Diseases
2021The role of epitranscriptomics, i.e., RNA base modification, as a component of a “methylome” in disease has emerged as a result of the development of next generation sequencing and other related state-of-art technologies. Epitranscriptomic state is controlled by writing, erasing, and reading methylation, which is mediated by enzymatic reactions.
Masamitsu Konno, Hideshi Ishii
openaire +1 more source
2022
Several chemical modifications in cellular RNAs have been identified to date. The most common internal modification of eukaryotic RNAs is known as m6A. This modification is able to configure the outcome of gene expression by adjusting RNA decay, translation efficiency, RNA structure and alternative splicing.
openaire +1 more source
Several chemical modifications in cellular RNAs have been identified to date. The most common internal modification of eukaryotic RNAs is known as m6A. This modification is able to configure the outcome of gene expression by adjusting RNA decay, translation efficiency, RNA structure and alternative splicing.
openaire +1 more source
Metabolic Regulation of the Epitranscriptome
ACS Chemical Biology, 2019An emergent theme in cancer biology is that dysregulated energy metabolism may directly influence oncogenic gene expression. This is due to the fact that many enzymes involved in gene regulation use cofactors derived from primary metabolism, including acetyl-CoA, S-adenosylmethionine, and 2-ketoglutarate.
Justin M. Thomas +2 more
openaire +2 more sources