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Epizootic Hemorrhagic Disease Agar Gel Immunodiffusion Assay.
Methods in molecular biologyAgar gel immunodiffusion assay (AGID) is a laboratory test which detects specific antigen-antibody interactions by the development of visible precipitation lines in a semisolid matrix. Here we describe the preparation of agar gel plates, the method to test serum samples by AGID for the presence of EHDV antibodies, and the interpretation of test results.
M. Torchetti +2 more
semanticscholar +3 more sources
Whole Genome Sequencing of Epizootic Hemorrhagic Disease Virus.
Methods in molecular biologyNext-generation sequencing (NGS) technologies are continuously being developed and are becoming a more cost-effective tool for the characterization of viral genomes. Whole genome sequencing of segmented viruses, such as epizootic hemorrhagic disease virus (EHDV), provides insights into the molecular epidemiology as well as such viral evolutionary ...
Simon King
semanticscholar +3 more sources
Infection of Culicoides Biting Midges with Epizootic Hemorrhagic Disease Virus.
Methods in molecular biologyEpizootic hemorrhagic disease virus (EHDV) is transmitted by Culicoides biting midges. Studies aiming to predict the likely spread of EHDV require an understanding of the viral infection and replication kinetics within these insects, including the proportion of the insect population that are able to support virus transmission. Here, we describe methods
Christopher Sanders, Kerry Newbrook
semanticscholar +3 more sources
EXPERIMENTAL INFECTION IN NORTH AMERICAN ELK WITH EPIZOOTIC HEMORRHAGIC DISEASE VIRUS [PDF]
Journal of Wildlife Diseases, 1973 Two elk (Cervus canadensis) were inoculated intravenously with epizootic hemorrhagic disease virus. No overt signs of disease were observed in the elk although one animal had a slight febrile response. A cell associated viremia was detected in both elk, which persisted in one animal for 30 days post-inoculation.
G L, Hoff, D O, Trainer
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Virus Isolation of Epizootic Hemorrhagic Disease Virus in Cell Culture.
Methods in molecular biologyVirus isolation is used to assist in the diagnosis and confirmation of viral infections. Successful isolation of a virus is highly dependent upon the quality of starting material. Here we describe the preparation and isolation of epizootic hemorrhagic disease virus (EHDV) from blood and tissue samples in tissue culture flasks (TCFs) through the ...
Amanda Corla
semanticscholar +3 more sources
Emergence of epizootic hemorrhagic disease in Europe
Virologie, 2023Epizootic hemorrhagic disease (EHD) is a non-contagious arthropod-borne disease transmitted by blood-sucking midges of the genus Culicoides. It affects domestic and wild ruminants, mainly white-tailed deer and cattle. At the end of October and in November 2022, outbreaks of EHD were confirmed in several cattle farms in Sardinia and Sicily.
Zientara, Stéphan +3 more
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Hemagglutination of epizootic hemorrhagic disease virus
Archives of Virology, 1981Hemagglutination of epizootic hemorrhagic disease virus (EHDV) with a variety of erythrocyte species at 4 degrees C, room temperature and 37 degrees C was dependent on the NaCl molarity and the pH of the diluent. The hemagglutination inhibition test was used to identify EHDV serotypes.
S, Tokuhisa +6 more
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Epizootic Hemorrhagic Disease in Alberta, Canada
Journal of Wildlife Diseases, 2014Epizootic hemorrhagic disease (EHD) virus serotype 2 was identified by reverse-transcription (RT)-PCR in a white-tailed deer (Odocoileus virginianus) found dead in southern Alberta in September 2013. Field observations indicate at least 50 deer, primarily white-tailed deer, and three pronghorn antelope (Antilocapra americana) died during a suspected ...
Margo J, Pybus +2 more
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Epizootic Hemorrhagic Disease Virus: Methods and Protocols
Methods in Molecular Biologysemanticscholar +2 more sources