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Molecular Serotyping of Epizootic Hemorrhagic Disease Virus
Molecular methods are routinely used for the differential diagnosis and genetic characterization of viral disease of livestock. Real-time, quantitative PCR (qPCR) allows RNA/DNA sequence detection and quantification and is considered the gold standard diagnostic method for most viruses.openaire +2 more sources
Whole Genome Sequencing of Epizootic Hemorrhagic Disease Virus
Next-generation sequencing (NGS) technologies are continuously being developed and are becoming a more cost-effective tool for the characterization of viral genomes. Whole genome sequencing of segmented viruses, such as epizootic hemorrhagic disease virus (EHDV), provides insights into the molecular epidemiology as well as such viral evolutionary ...openaire +2 more sources
Infection of Culicoides Biting Midges with Epizootic Hemorrhagic Disease Virus
Epizootic hemorrhagic disease virus (EHDV) is transmitted by Culicoides biting midges. Studies aiming to predict the likely spread of EHDV require an understanding of the viral infection and replication kinetics within these insects, including the proportion of the insect population that are able to support virus transmission. Here, we describe methodsChristopher, Sanders, Kerry, Newbrook
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Epizootic Hemorrhagic Disease of Deer
The Journal of Wildlife Management, 1964openaire +1 more source
Epizootic Hemorrhagic Disease in White-tailed Deer from Missouri
Journal of Wildlife Diseases, 1983R E, Brannian +3 more
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Virus Isolation of Epizootic Hemorrhagic Disease Virus in Cell Culture
Virus isolation is used to assist in the diagnosis and confirmation of viral infections. Successful isolation of a virus is highly dependent upon the quality of starting material. Here we describe the preparation and isolation of epizootic hemorrhagic disease virus (EHDV) from blood and tissue samples in tissue culture flasks (TCFs) through the ...openaire +2 more sources