Results 191 to 200 of about 6,579 (227)

Experimental infection of calves with epizootic hemorrhagic disease virus

American Journal of Veterinary Research, 1999
Abstract Objective To determine whether experimental inoculation with a field strain of epizootic hemorrhagic disease virus serotype-2 (EHDV-2) suspected of causing clinical disease in naturally infected cattle would cause clinical disease in calves. Animals 8 calves.
M J, Abdy, E E, Howerth, D E, Stallknecht   +2 more
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Clinical disease associated with epizootic hemorrhagic disease virus in cattle in Illinois

Journal of the American Veterinary Medical Association, 2015
Abstract Objective—To describe the clinical manifestation of epizootic hemorrhagic disease virus (EHDV) in cattle during an outbreak in northwestern Illinois in the fall of 2013. Design—Outbreak survey. Sample—18 EHDV-affected cattle herds. Procedures—Herds with confirmed EHDV-infected cattle were selected for the survey on the basis of having a ...
Andrew M. Hubner, Suzette K. Hexum, Elena Bichi, Eleonora Po, Edgar F. Garrett, Robert Melcher   +5 more
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Review of epizootic hemorrhagic disease in cattle and a study defining seroprevalence of epizootic hemorrhagic disease virus serotype 2 in Texas cattle

The Bovine Practitioner, 2018
Epizootic hemorrhagic disease virus is an orbivirus transmitted by Culicoides spp. In North America, it is recognized as a major cause of disease affecting white-tailed deer, but it can impact other wildlife species as well as domestic cattle. Although minimal clinical disease had been noted in Texas, periodic outbreaks in other states caused Texas ...
Thomas B. Hairgrove, Walter M. Cook, William B Smith, Christine M. Budke, Sandy M. Rodgers   +4 more
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Epizootic hemorrhagic disease virus of deer: an electron microscopic study

Canadian Journal of Microbiology, 1970
A mouse brain adapted strain of epizootic hemorrhagic disease (EHD) virus of deer was studied by ultrathin section and negative contrast electron microscopy. In sections of infected BHK-21 cells and neurons of newborn mouse brains, aggregates of virus particles, some appearing to be lacking their outer coats, were observed within membrane-bound ...
Lars Karstad, Kin-Son Tsai
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Epizootic Hemorrhagic Disease Virus in Montana: Isolation and Serologic Survey

American Journal of Veterinary Research, 1981
SUMMARY Epizootic hemorrhagic disease virus (ehdv) was isolated in Vero cell culture from the spleen and whole blood of a white-tailed deer (Odocoileus virginianus). A 10% spleen suspension caused acute hemorrhagic disease (hd) when inoculated into an experimental white-tailed deer and resulted in the recovery of ehdv from the blood of the experimental
T J, Feldner, M H, Smith
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Epizootic Hemorrhagic Disease Virus Titration

The titration of viruses onto susceptible cell lines is an important virological technique used to quantify infectious viral titers. It forms an integral component of epizootic hemorrhagic disease virus (EHDV) research, including estimating infectivity, calculating multiplicity of infection, and confirming virus propagation in cell culture.
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EXPERIMENTAL INFECTION IN NORTH AMERICAN ELK WITH EPIZOOTIC HEMORRHAGIC DISEASE VIRUS

Journal of Wildlife Diseases, 1973
Two elk (Cervus canadensis) were inoculated intravenously with epizootic hemorrhagic disease virus. No overt signs of disease were observed in the elk although one animal had a slight febrile response. A cell associated viremia was detected in both elk, which persisted in one animal for 30 days post-inoculation.
Gerald L. Hoff, Daniel O. Trainer
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Epizootic Hemorrhagic Disease Agar Gel Immunodiffusion Assay

Agar gel immunodiffusion assay (AGID) is a laboratory test which detects specific antigen-antibody interactions by the development of visible precipitation lines in a semisolid matrix. Here we describe the preparation of agar gel plates, the method to test serum samples by AGID for the presence of EHDV antibodies, and the interpretation of test results.
Mia Kim, Torchetti, Abaineh, Endalew, Albert, Van Geelen   +2 more
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Molecular Serotyping of Epizootic Hemorrhagic Disease Virus

Molecular methods are routinely used for the differential diagnosis and genetic characterization of viral disease of livestock. Real-time, quantitative PCR (qPCR) allows RNA/DNA sequence detection and quantification and is considered the gold standard diagnostic method for most viruses.
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Development and optimization of a hybridization assay for epizootic hemorrhagic disease viruses

Journal of Virological Methods, 1990
Hybridization assays for the double-stranded RNA (dsRNA) orbiviruses causing bluetongue and epizootic hemorrhagic disease are more labor-intensive and less sensitive than enzyme-linked immunosorbent assay (ELISA) and immunoperoxidase assay. Cell-culture EHD virus amplification, rapid extraction, and optimization of RNA blotting conditions were examined
openaire   +3 more sources