Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Spatial competition and equilibrium in a circular market [PDF]
The paper presents an analysis of spatial competition between two firms in a circular market. It is shown that the existence problem of a Bertrand-Nash- Equilibrium exists for the circular market in the same way as for the linear and bounded market. This
Kopp, Andreas
core
Assisting Forearm Function in Children With Movement Disorders via A Soft Wearable Robot With Equilibrium-Point Control. [PDF]
Front Robot AI, 2022 Realmuto J, Sanger TD.
europepmc +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Existence of pseudo-equilibria in a financial economy [PDF]
This paper proves the existence of a pseudo-equilibrium in a financial economy with incomplete markets in which the agents may have nonordered preferences.
Bernard Cornet, Philippe Bich
core +2 more sources
Multiple time scales method for a two dimensional system with distributed delays
Annals of the West University of Timisoara: Mathematics and Computer ScienceBuilding on the simplified mathematical model derived from the dynamics of the hypothalamic-pituitary-adrenal (HPA) axis, which describes the interactions between ACTH and cortisol through a system of differential equations with distributed delays, this ...
Matei Maria Roxana
doaj +1 more source
Analysis on the dynamic evolution of the equilibrium point of "carbon emission penetration" for energy-intensive industries in China: based on a factor-driven perspective. [PDF]
Environ Sci Pollut Res Int, 2023 Liu J, Wei D.
europepmc +1 more source
Games with Equilibrium Points [PDF]
Proceedings of the National Academy of Sciences, 1953 Otter, Richard, Dunne, John
openaire +3 more sources
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Novel adaptive generalized principal component analysis algorithm based on Hebbian rule
Tongxin xuebao, 2020 In order to adaptively estimate the generalized principal component from input signals,a novel generalized principal component analysis algorithm was proposed based on the Hebbian linear neuron model.Since the autocorrelation matrices of the signals were
Yingbin GAO +3 more
doaj