Results 71 to 80 of about 315,290 (261)
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
SELECTION OF EQUIPMENT AND TECHNOLOGICAL EQUIPMENT
The issues of acquiring practical skills in the selection of technological equipment, tools, cutting and measuring tools were considered. Depending on the volume of production, machines with a high level of specialization and high productivity, as well as machines with numerical control (NCC), are selected.
openaire +2 more sources
FEBS Open Bio, EarlyView.Bioscience students were asked for their opinions on the value and teaching of skills. 204 responded that teamwork, time management and study skills are necessary to reach University, that scientific writing, research, laboratory and presentation skills are taught effectively during their studies, while other skills are gained inherently through study ...
Janella Borrell, Susan Crennell
wiley +1 more source
Journal of Engineering, 2016 Engineering equipment is essential part in the construction project and usually manufactured with long lead times, large costs and special engineering requirements.
Kadhim Raheem Erzaij, Dr +1 more
doaj +2 more sources
MATEC Web of Conferences, 2017 Today’s construction projects are highly mechanized. That#x2019;s why selection of right equipment has always been a key factor in the success of any construction project.
Romanov Petr, Romanova Irina
doaj +1 more source
Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
FEBS Open Bio, EarlyView.Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source
YIPFα1A expression is regulated by multilayered molecular mechanisms
FEBS Open Bio, EarlyView.YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji +2 more
wiley +1 more source
Sustainable port maintenance: Dredging equipment selection in time-emission trade-offs
Results in EngineeringMaintenance dredging in ports and waterways is essential to ensure safe navigation. With increasing regulatory pressure on the maritime sector to reduce exhaust emissions, both dredging contractors and port authorities are seeking effective mitigation ...
Arash Sepehri +4 more
doaj +1 more source
Controversies in Respiratory Protective Equipment Selection and Use During COVID-19. [PDF]
J Hosp Med, 2020 Zhang JC, Cram P, Adisesh A.
europepmc +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
FEBS Open Bio, EarlyView.RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source