Results 111 to 120 of about 2,341,583 (290)

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Morgan-Voyce Polynomial Approach for Solution of High-Order Linear Differential-Difference Equations with Residual Error Estimation

Düzce Üniversitesi Bilim ve Teknoloji Dergisi, 2016
The main aim of this study is to apply the Morgan-Voyce polynomials for the solution of high-order linear differential difference equations with functional arguments under initial boundary conditions.
Bengü Türkyılmaz, Burcu Gürbüz, Mehmet Sezer   +2 more
doaj  

Evaluation of in vitro toxicity of common phytochemicals included in weight loss supplements using 1H NMR spectroscopy

FEBS Open Bio, EarlyView.
We investigated the toxicity of 12 active compounds commonly found in herbal weight loss supplements (WLS) using human liver and colon cell models. Epigallocatechin‐3‐gallate was the only compound showing significant toxicity. Metabolic profiling revealed protein degradation, disrupted energy and lipid metabolism suggesting that the inclusion of EGCG ...
Emily C. Davies, Garth L. Maker, Ian F. Musgrave, Samantha Lodge   +3 more
wiley   +1 more source

Automatic Refractive Error Estimation Using Deep Learning-Based Analysis of Red Reflex Images. [PDF]

Diagnostics (Basel), 2023
Linde G, Chalakkal R, Zhou L, Huang JL, O'Keeffe B, Shah D, Davidson S, Hong SC.   +7 more
europepmc   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Pedestrian Localization with Stride-Wise Error Estimation and Compensation by Fusion of UWB and IMU Data. [PDF]

Sensors (Basel), 2023
Hölzke F, Borstell H, Golatowski F, Haubelt C.   +3 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Wavelet Based Semi-blind Channel Estimation For Multiband OFDM

, 2007
This paper introduces an expectation-maximization (EM) algorithm within a wavelet domain Bayesian framework for semi-blind channel estimation of multiband OFDM based UWB communications.
Duhamel, Pierre, Ichir, Mahieddine, Jaffrot, Emmanuel, Sadough, Sajad   +3 more
core   +3 more sources

A fast MR fingerprinting simulator for direct error estimation and sequence optimization. [PDF]

Magn Reson Imaging, 2023
Hu S, Jordan S, Boyacioglu R, Rozada I, Troyer M, Griswold M, McGivney D, Ma D.   +7 more
europepmc   +1 more source