Results 161 to 170 of about 88,006 (195)
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Retention of Potassium by Human Erythrocyte Ghosts
Nature, 1960WHEN ghosts prepared from human erythrocytes by lysis in water are washed in a saline medium, sodium and potassium ions are nearly uniformly distributed across the ghost membranes1. In attempting to prepare ghosts with higher potassium and lower sodium concentrations than those of a saline medium we have found that incubation at 37° C.
J F, HOFFMAN, D C, TOSTESON, R, WHITTAM
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The role of spectrin in erythrocyte ghost endocytosis
Biochemical and Biophysical Research Communications, 1978Abstract Endocytosis in white ghosts prepared from human erythrocytes was induced by three methods: incubation with Mg-ATP, incubation with 0.1 mM EDTA, and digestion with 20 nanograms (ng.) per ml. of trypsin. In each case the endocytic vacuoles that were produced when separated and analyzed on SDS-polyacrylamide gel electrophoresis were found to be
B, Hardy, S L, Schrier
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Modification of human erythrocyte ghosts with transglutaminase
Biochemical and Biophysical Research Communications, 1975Abstract Guinea pig liver transglutaminase was shown to catalyze the incorporation of dansylcadaverine and putrescine into two major protein fractions of human erythrocyte ghosts. As judged by sodium dodecylsulfate gel electrophoresis under reducing conditions, one of these is a high molecular weight polymer which may contain spectrin.
L, Lorand +3 more
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Thiamin transport by human erythrocytes and ghosts
The Journal of Membrane Biology, 1990Thiamin transport in human erythrocytes and resealed pink ghosts was evaluated by incubating both preparations at 37 or 20 degrees C in the presence of [3H]-thiamin of high specific activity. The rate of uptake was consistently higher in erythrocytes than in ghosts. In both preparations, the time course of uptake was independent from the presence of Na+
D, Casirola +3 more
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Carbonic anhydrase activity of erythrocyte ghosts
Experientia, 1968Durch wiederholtes Waschen mit hypotonen Losungen gelingt es, mit dem Hamoglobin auch die Carboanhydrase vollstandig aus Erythrocytenstromata zu entfernen.
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The kinetics of resealing of washed erythrocyte ghosts
The Journal of Membrane Biology, 1975The kinetics of resealing, defined as the recovery of impermeability to macromolecules, of well-washed human erythrocyte ghosts has been determined. The resealing process is first-order at temperatures above 20-25 degrees C in isotonic salt, with rate constants ranging from 0.01-0.15 min-1. Below 20 degrees C, resealing occurs, but a long lag period is
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Endocytosis in erythrocytes and their ghosts.
Progress in clinical and biological research, 1980Study of endocytosis in human erythrocytes and their ghosts provides an opportunity for the elucidation of the steps involved in membrane invagination and fusion. The specific preparation being studied should be described with great care since circumstances that produce endocytosis may differ among the several available preparations.
S L, Schrier, B, Hardy, K G, Bensch
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Membrane Perturbations of Erythrocyte Ghosts by Spectrin Release
The Journal of Biochemistry, 2007The cytoskeleton plays an important role in the stability and function of the membrane. Spectrin release from erythrocyte ghosts makes the membrane more fragile. However, the detail of membrane fragility has remained unclear. In the present study, the effects of incubation temperatures and polyamines on the membrane structure of ghosts under hypotonic ...
Takeo, Yamaguchi +3 more
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PHOTOCHEMICAL PROPERTIES OF ERYTHROCYTE GHOSTS CONTAINING PORPHYRIN
Photochemistry and Photobiology, 1988Abstract— Rabbit erythrocyte cell ghosts were prepared containing tetrasodium meso‐tetra (4‐sulfona‐to‐phenyl) porphine (12‐hydrate), a water soluble porphyrin. Laser flash kinetic spectroscopy of the suspension revealed a difference spectrum consistent with the porphyrin T, state.
P A, Firey, M A, Rodgers
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Use of Erythrocyte Ghosts for Preconcentration in Element Speciation
Analytical Chemistry, 1999A totally new biosorption concept has been tested. It involves the use of a biological membrane with given transport characteristics that encloses a reaction compartment chosen to bind incorporated substances irreversibly. Such a system is an erythrocyte ghost: erythrocytes that are hypotonically lysed can be filled with the desired media and resealed ...
O, Rohling, B, Neidhart
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