Results 171 to 180 of about 4,783 (213)
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Retention of Potassium by Human Erythrocyte Ghosts
Nature, 1960WHEN ghosts prepared from human erythrocytes by lysis in water are washed in a saline medium, sodium and potassium ions are nearly uniformly distributed across the ghost membranes1. In attempting to prepare ghosts with higher potassium and lower sodium concentrations than those of a saline medium we have found that incubation at 37° C.
J F, HOFFMAN, D C, TOSTESON, R, WHITTAM
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Metabolism of deoxyinosine by human erythrocyte ghosts
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1966Abstract Deoxyinosine was metabolized by human erythrocyte ghosts. Kinetic studies revealed an accumulation of hypoxanthine, a decrease in deoxypentose, and an initial uptake of inorganic phosphate followed by its eventual release. Intermediates of the pathway identified were triose phosphate (glyceraldehyde 3-phosphate and dihydroxyacetone phosphate)
F J, Lionetti, N L, Fortier
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The role of spectrin in erythrocyte ghost endocytosis
Biochemical and Biophysical Research Communications, 1978Abstract Endocytosis in white ghosts prepared from human erythrocytes was induced by three methods: incubation with Mg-ATP, incubation with 0.1 mM EDTA, and digestion with 20 nanograms (ng.) per ml. of trypsin. In each case the endocytic vacuoles that were produced when separated and analyzed on SDS-polyacrylamide gel electrophoresis were found to be
B, Hardy, S L, Schrier
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Thiamin transport by human erythrocytes and ghosts
The Journal of Membrane Biology, 1990Thiamin transport in human erythrocytes and resealed pink ghosts was evaluated by incubating both preparations at 37 or 20 degrees C in the presence of [3H]-thiamin of high specific activity. The rate of uptake was consistently higher in erythrocytes than in ghosts. In both preparations, the time course of uptake was independent from the presence of Na+
D, Casirola +3 more
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The kinetics of resealing of washed erythrocyte ghosts
The Journal of Membrane Biology, 1975The kinetics of resealing, defined as the recovery of impermeability to macromolecules, of well-washed human erythrocyte ghosts has been determined. The resealing process is first-order at temperatures above 20-25 degrees C in isotonic salt, with rate constants ranging from 0.01-0.15 min-1. Below 20 degrees C, resealing occurs, but a long lag period is
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Endocytosis in erythrocytes and their ghosts.
Progress in clinical and biological research, 1980Study of endocytosis in human erythrocytes and their ghosts provides an opportunity for the elucidation of the steps involved in membrane invagination and fusion. The specific preparation being studied should be described with great care since circumstances that produce endocytosis may differ among the several available preparations.
S L, Schrier, B, Hardy, K G, Bensch
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PHOTOCHEMICAL PROPERTIES OF ERYTHROCYTE GHOSTS CONTAINING PORPHYRIN
Photochemistry and Photobiology, 1988Abstract— Rabbit erythrocyte cell ghosts were prepared containing tetrasodium meso‐tetra (4‐sulfona‐to‐phenyl) porphine (12‐hydrate), a water soluble porphyrin. Laser flash kinetic spectroscopy of the suspension revealed a difference spectrum consistent with the porphyrin T, state.
P A, Firey, M A, Rodgers
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Carbonic anhydrase activity of erythrocyte ghosts
Experientia, 1968Durch wiederholtes Waschen mit hypotonen Losungen gelingt es, mit dem Hamoglobin auch die Carboanhydrase vollstandig aus Erythrocytenstromata zu entfernen.
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Raman and resonance-Raman scattering by erythrocyte ghosts
Biochimica et Biophysica Acta (BBA) - Biomembranes, 19751. We present the laser-Raman spectra of human erythrocyte ghosts, isolated by standard conditions and compare these with the spectra of lecithin liposomes and fat-free serum albumin. 2. The hydrocarbon stretching modes of membrane lipids are temperature sensitive and may serve as a index of hydrocarbon chain motion. 3.
D F, Wallach, S P, Verma
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Membrane expansion of erythrocyte ghosts by tranquilizers and anesthetics
Biochimica et Biophysica Acta (BBA) - Biomembranes, 19691. 1. The ghosts of erythrocytes, hemolyzed in 0.3 % NaCl (pH 7) and in a final hematocrit of 0.002 %, appeared to be spherical under phase contrast microscopy. From photographic measurements of the diameter, the mean area of the erythrocytes was found to be 134.8 μ2. 2. 2.
P, Seeman, W O, Kwant, T, Sauks
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