Results 101 to 110 of about 1,293,873 (407)
Rainbow Vectors for Broad-Range Bacterial Fluorescence Labeling.
PLoS ONE, 2016 Since their discovery, fluorescent proteins have been widely used to study protein function, localization or interaction, promoter activity and regulation, drug discovery or for non-invasive imaging.
Mariette Barbier, F Heath Damron
doaj +1 more source
Remarkable diversity in the enzymes catalyzing the last step in synthesis of the pimelate moiety of biotin. [PDF]
PLoS ONE, 2012 Biotin synthesis in Escherichia coli requires the functions of the bioH and bioC genes to synthesize the precursor pimelate moiety by use of a modified fatty acid biosynthesis pathway.
Madelyn M Shapiro +2 more
doaj +1 more source
YoeB toxin is activated during thermal stress. [PDF]
, 2015 Type II toxin-antitoxin (TA) modules are thought to mediate stress-responses by temporarily suppressing protein synthesis while cells redirect transcription to adapt to environmental change.
Garza-Sánchez, Fernando +2 more
core +1 more source
SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
FEBS Open Bio, EarlyView.The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
PhoU homologs from Staphylococcus aureus dimerization and protein interactions
Microbiology SpectrumPhoU proteins are negative regulators of the phosphate response, regulate virulence, and contribute to antibiotic resistance. Staphylococcus aureus has multiple genes encoding PhoU homologs that regulate persister formation and potentially virulence, but
Clayton T. Matthews +2 more
doaj +1 more source
Expression and Purification of Mini G Proteins from Escherichia coli
Bio-Protocol, 2017 Heterotrimeric G proteins modulate intracellular signalling by transducing information from cell surface G protein-coupled receptors (GPCRs) to cytoplasmic effector proteins.
Byron Carpenter, Christopher Tate
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Replication of plasmids derived from Shiga toxinconverting bacteriophages in starved Escherichia coli [PDF]
, 2011 The pathogenicity of Shiga toxin-producing Escherichia coli (STEC) depends on the expression of stx genes that are located on lambdoid prophages. Effective toxin production occurs only after prophage induction, and one may presume that replication of the
Nadratowska-Wesołowska, Beata +4 more
core
Gene doctoring: a method for recombineering in laboratory and pathogenic Escherichia coli strains [PDF]
, 2009 Background: Homologous recombination mediated by the lambda-Red genes is a common method for making chromosomal modifications in Escherichia coli.
Bingle, Lewis EH +6 more
core +5 more sources
Universal Stress Proteins inEscherichia coli [PDF]
Journal of Bacteriology, 2005 Multiple members of the UspA family of proteins are found in the genomes of bacteria, archaea, fungi, protozoa, and plants, but the biological and biochemical functions of these proteins are not known. In this issue of Journal of Bacteriology, Nachin et al.
openaire +2 more sources
FEBS Open Bio, EarlyView.dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon +6 more
wiley +1 more source