Conservation and global distribution of non-canonical antigens in enterotoxigenic Escherichia coli [PDF]
BACKGROUND: Enterotoxigenic Escherichia coli (ETEC) cause significant diarrheal morbidity and mortality in children of resource-limited regions, warranting development of effective vaccine strategies.
Boxrud, Dave+14 more
core +2 more sources
Lipid bilayer composition influences small multidrug transporters [PDF]
Background: Membrane proteins are influenced by their surrounding lipids. We investigate the effect of bilayer composition on the membrane transport activity of two members of the small multidrug resistance family; the Escherichia coli transporter, EmrE
Booth, P.J.+3 more
core +3 more sources
Immunolabeling of Proteins in situ in Escherichia coli K12 Strains
This protocol was developed to label proteins in bacterial cells with antibodies conjugated to a fluorophore for fluorescence microscopy imaging. The procedure is optimized to minimize morphological changes and also to minimize the amount of antibodies ...
Nienke Buddelmeijer+2 more
doaj +1 more source
Rational design of a plasmid origin that replicates efficiently in both gram-positive and gram-negative bacteria. [PDF]
Most plasmids replicate only within a particular genus or family.Here we describe an engineered high copy number expression vector, pBAV1K-T5, that produces varying quantities of active reporter proteins in Escherichia coli, Acinetobacter baylyi ADP1 ...
Anton V Bryksin, Ichiro Matsumura
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E. coli and colon cancer : Is mutY a culprit? [PDF]
Peer ...
Cash, Phillip, Khan, Abdul Arif
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Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins.
One-third of the 4,225 protein-coding genes of Escherichia coli K-12 remain functionally unannotated (orphans). Many map to distant clades such as Archaea, suggesting involvement in basic prokaryotic traits, whereas others appear restricted to E.
Pingzhao Hu+22 more
doaj +1 more source
Remarkable diversity in the enzymes catalyzing the last step in synthesis of the pimelate moiety of biotin. [PDF]
Biotin synthesis in Escherichia coli requires the functions of the bioH and bioC genes to synthesize the precursor pimelate moiety by use of a modified fatty acid biosynthesis pathway.
Madelyn M Shapiro+2 more
doaj +1 more source
Assembly of membrane proteins in Escherichia coli.
1980, 255:802-806.€ Access the most updated version of this article at http://www.jbc.org/content/255/2/802.citation€ Find articles, minireviews, Reflections and Classics on similar topics on the JBC Affinity Sites.Alerts: €€ •€ When a correction for this article is posted •€ When this article is cited Click here to choose from all of JBC's e-mail ...
H C Wu, J J Lin
openaire +3 more sources
PhoU homologs from Staphylococcus aureus dimerization and protein interactions
PhoU proteins are negative regulators of the phosphate response, regulate virulence, and contribute to antibiotic resistance. Staphylococcus aureus has multiple genes encoding PhoU homologs that regulate persister formation and potentially virulence, but
Clayton T. Matthews+2 more
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Recombinant protein expression in Escherichia coli: advances and challenges
Escherichia coli is the organism of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform.
Germán Leandro Rosano+3 more
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