Characterisation of the effects of salicylidene acylhydrazide compounds on type three secretion in Escherichia coli O157:H7 [PDF]
, 2009 Recent work has highlighted a number of compounds that target bacterial virulence by affecting gene regulation. In this work, we show that small-molecule inhibitors affect the expression of the type III secretion system (T3SS) of <i>Escherichia ...
Elofsson, M. +9 more
core +3 more sources
Expression and Purification of Mini G Proteins from Escherichia coli
Bio-Protocol, 2017 Heterotrimeric G proteins modulate intracellular signalling by transducing information from cell surface G protein-coupled receptors (GPCRs) to cytoplasmic effector proteins.
Byron Carpenter, Christopher Tate
doaj +1 more source
The quantitative and condition-dependent Escherichia coli proteome
Nature Biotechnology, 2015 Measuring precise concentrations of proteins can provide insights into biological processes. Here we use efficient protein extraction and sample fractionation, as well as state-of-the-art quantitative mass spectrometry techniques to generate a ...
A. Schmidt +9 more
semanticscholar +1 more source
Identification of the YfgF MASE1 domain as a modulator of bacterial responses to aspartate [PDF]
, 2013 Complex 3'-5'-cyclic diguanylic acid (c-di-GMP) responsive regulatory networks that are modulated by the action of multiple diguanylate cyclases (DGC; GGDEF domain proteins) and phosphodiesterases (PDE; EAL domain proteins) have evolved in many bacteria.
Agasing, A. +3 more
core +1 more source
PhoU homologs from Staphylococcus aureus dimerization and protein interactions
Microbiology SpectrumPhoU proteins are negative regulators of the phosphate response, regulate virulence, and contribute to antibiotic resistance. Staphylococcus aureus has multiple genes encoding PhoU homologs that regulate persister formation and potentially virulence, but
Clayton T. Matthews +2 more
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Rational design of a plasmid origin that replicates efficiently in both gram-positive and gram-negative bacteria. [PDF]
PLoS ONE, 2010 Most plasmids replicate only within a particular genus or family.Here we describe an engineered high copy number expression vector, pBAV1K-T5, that produces varying quantities of active reporter proteins in Escherichia coli, Acinetobacter baylyi ADP1 ...
Anton V Bryksin, Ichiro Matsumura
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In vitro starch binding experiments: Study of the proteins related to grain hardness of wheat [PDF]
, 2007 Two friabilin components, puroindoline a and GSP-1 were expressed in Escherichia coli. Starch binding properties of the recombinant polypeptides and of friabilin extracted from wheat flour were compared in vitro. The produced proteins as well as native
Bakó, Ambrus +2 more
core
Open Biology, 2016 The ease of genetic manipulation, low cost, rapid growth and number of previous studies have made Escherichia coli one of the most widely used microorganism species for producing recombinant proteins.
Baolei Jia, C. Jeon
semanticscholar +1 more source
Mutations that Separate the Functions of the Proofreading Subunit of the Escherichia coli Replicase
, 2015 The dnaQ gene of Escherichia coli encodes the Ɛ subunit of DNA polymerase III, which provides the 3\u27 - 5\u27 exonuclease proofreading activity of the replicative polymerase.
Kreuzer, Kenneth N., Whatley, Zakiya
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Recombinant human preproinsulin expression, purification and reaction with insulin autoantibodies in sera from patients with insulin-dependent diabetes mellitus [PDF]
, 1993 A novel prokaryotic expression vector pGEX-6T was designed for high-level expression of recombinant fusion protein with a histidine-hexapeptide and glutathione-S-transferase at its N-terminus and the recombinant human preproinsulin at its C-terminus ...
Amman +29 more
core +1 more source