Results 91 to 100 of about 55,913 (214)

Comparative evaluation of noncanonical amino acids as site‐specific NMR probes for the complex of E. coli SSB with single‐stranded DNA without isotope labelling

The FEBS Journal, EarlyView.
Genetic encoding of noncanonical amino acids (ncAA) enables their site‐specific installation in proteins. This work compares the nuclear magnetic resonance (NMR) performance of different ncAAs in a large protein−DNA complex (~ 100 kDa) without isotope labelling. The ncAAs deliver readily detectable NMR signals in the complex between E.
Sreelakshmi Mekkattu Tharayil, Haocheng Qianzhu, Elwy H. Abdelkader, Adarshi P. Welegedara, Josemon George, Christoph Nitsche, Thomas Huber, Gottfried Otting   +7 more
wiley   +1 more source

Hsp70 diversification and repurposing across the tree of life: Lessons from the evolutionary and mechanistic trajectory of the Hsp70–Hsp110 chaperone system

The FEBS Journal, EarlyView.
Evolutionary and mechanistic divergence in the Hsp70–Hsp110 chaperone system. Prokaryotic Hsp70s probably diversified into multiple orthologues that cooperated with co‐chaperones such as JDPs and NEF, forming increasingly complex proteostasis networks.
Pierre Goloubinoff, Paolo De Los Rios, Mathieu E. Rebeaud   +2 more
wiley   +1 more source

Recombinant Fibrinogen-Gamma-Chain as a Crosslinker of Thiol-ene Hydrogels. [PDF]

Biopolymers
Fibrinogen gamma chain (FGG) was recombinantly expressed in E. coli to generate a recombinant protein containing free thiol groups from cysteine. The expressed protein was used to crosslink norbornene functionalized gelatin providing a proof‐of‐principle of this novel crosslinker type and the formed hydrogels were characterized for their stiffness ...
Schlauch D, Güler CS, Harzi M, Kara S, Lavrentieva A, Pepelanova I.   +5 more
europepmc   +2 more sources

A distinct gene expression mechanism for d‐amino acid utilization in hyperthermophilic archaeon Pyrococcus horikoshii

The FEBS Journal, EarlyView.
The genes PH0137, PH0138, and PH0140 form a cluster in the genome of Pyrococcus horikoshii. The PH0140 gene encodes a transcription factor identified as a d‐amino acid‐responsive regulatory protein (DARP). In feast mode, l‐Ile‐bound DARP binds to the promoter and represses transcription of PH0137 and PH0138. In famine mode, DARP binds to d‐allo‐Ile and
Ryushi Kawakami, Tomoki Kawase, Taira Uehara, Haruhiko Sakuraba, Tomohiko Matsuzawa, Junji Hayashi, Toshihisa Ohshima   +6 more
wiley   +1 more source

Aptamer‐mediated outer membrane destabilization overcomes low permeability resistance in Pseudomonas aeruginosa

The FEBS Journal, EarlyView.
DNA aptamers, Apt 60 and Apt 173, target the BamA protein to compromise outer membrane integrity in Pseudomonas aeruginosa, making it susceptible to Azithromycin, an antibiotic that is otherwise poorly effective due to the low‐permeability membrane.
Rupany Selvam, Michelle Khai Khun Yap, Rafael Giacomazzi, Sérgio F. Sousa, Hock Siew Tan   +4 more
wiley   +1 more source

Unraveling in vitro phase separation and aggregation properties of the structured region of FMRP and the impact of Fragile X syndrome‐linked mutations

The FEBS Journal, EarlyView.
Fragile X messenger ribonucleoprotein 1 (FMRP) is a multidomain RNA‐binding protein associated with Fragile X Syndrome (FXS). We found that its N‐terminal structured region has an intrinsic propensity to undergo liquid–liquid phase separation and fibril formation. FXS‐associated mutations perturb protein stability and aggregation propensity, suggesting
Flavia Catalano, Alessandra Bigi, Francesca Troilo, Federica Gabriele, Gianluca Pistoia, Italia A. Asteriti, Francesco Angelucci, Giorgio Giardina, Fabrizio Chiti, Carlo Travaglini‐Allocatelli, Adele Di Matteo   +10 more
wiley   +1 more source

Structural and thermodynamic analyses of a novel β‐1,2‐glucan binding mode in the ABC transporter solute‐binding protein Chy400_4166 from Chloroflexus aurantiacus

The FEBS Journal, EarlyView.
Cyclic β‐1,2‐glucan (CβG)‐associated solute‐binding protein of type I ABC transporter was originally identified in Chloroflexus aurantiacus, a phototrophic bacterium. Crystal structures in complex with CβGs revealed unique binding modes that are fundamentally distinct from that of the short β‐1,2‐glucooligosaccharides‐binding protein.
Kazuya Kato, Tatsuya Kaneko, Rintaro Hirayama, Nobukiyo Tanaka, Hiroyuki Nakai, Hidetaka Torigoe, Masahiro Nakajima   +6 more
wiley   +1 more source

Coniferyl aldehyde from the phenylpropanoid pathway targets pyruvate kinase in Dactylobotrys graminicola to confer sheath rot resistance in hulless barley

Journal of Integrative Plant Biology, EarlyView.
Hulless barley resists the newly identified sheath rot disease caused by the necrotrophic fungal pathogen Dactylobotrys graminicola by releasing coniferyl aldehyde, a natural compound that disrupts energy production in the fungus. ABSTRACT Dactylobotrys graminicola (Dgr), a necrotrophic fungal pathogen, has recently been identified as the causative ...
Haowen Zheng, Liyifan Chen, Jianguo Wei, Yuandi Xu, Rangrang Chen, Yu Li, Yan Liu, Jiahui Yan, Lu Hou, Yiliang Chen, Zhensheng Kang, Haifeng Zhang, Qiang Yao   +12 more
wiley   +1 more source

Polar localization of putative phospholipid transporters in <i>Escherichia coli</i>. [PDF]

mBio
Tan WB, Chong Z-S, Cheong JWM, Yeow J, Chng S-S.   +4 more
europepmc   +1 more source

Built different: ER cisternae formed by the Arabidopsis Lunapark proteins differ in ultrastructure and affect ER–Golgi transport

New Phytologist, EarlyView.
The endoplasmic reticulum (ER) is a key structure in plant cells that helps produce and transport proteins. We discovered that different Lunapark proteins shape ER structures in distinct ways, affecting protein movement and secretion. Understanding how ER structure influences function can help improve plant‐based production of valuable compounds, such ...
Charlotte Pain, Tatiana Spatola Rossi, Nadine Field, Carmen Mata, Alessia Candeo, Muhammad W. Ali, Flavia Moreira‐Leite, Stanley W. Botchway, Federica Brandizzi, Verena Kriechbaumer   +9 more
wiley   +1 more source