RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj +3 more
wiley +1 more source
Antibacterial Properties of Ajuga chamaecistus Subsp. Scoparia and Chemical Composition of its Oils [PDF]
In the present study, we reported the essential oils chemical composition and antibacterial activities of the aerial parts of Ajuga chamaecistus Ging. ex Benth. Subsp. Scoparia (Boiss.) Rech.f.
Abdolrasoul Haghir Ebrahimabadi +3 more
doaj
Effect of sucrose replacement on physicochemical parameters and sensory characteristics of instant coffee drinks [PDF]
Coffee is one of the most popular drinks in the world. There has been a trend in consumer behaviour over the last few years - a change in the traditional way of consuming coffee.
Hadjikinova Raina, Petrova Ivanka
doaj +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
On the conversion of the essential oil of mustard into the essential oil of garlic [PDF]
(1845). On the conversion of the essential oil of mustard into the essential oil of garlic. The London, Edinburgh, and Dublin Philosophical Magazine and Journal of Science: Vol. 26, Supplement, pp. 616-618.
openaire +2 more sources
Lipid components and oxidative status of selected specialty oils [PDF]
Many vegetable oils are marketed as specialty oils because of their retained flavors, tastes and distinct characteristics. Specialty oil samples which were commercially produced and retailed were purchased from local superstores in Reading, UK ...
Dutta, Paresh +8 more
core +1 more source
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya +2 more
wiley +1 more source
Chemical Composition and in Vitro Evaluation of the Antioxidant and Antimicrobial Activities of Eucalyptus gillii Essential Oil and Extracts [PDF]
In this study, essential oil and various extracts (hexane, petroleum ether, acetone, ethanol, methanol and water) of Eucalyptus gilii were screened for their chemical composition, antimicrobial and antioxidant activities.
Abderrabba, Manef +13 more
core +1 more source

