PCL enzymatic hydrolysis: a mechanistic study [PDF]
Accumulation of plastic waste is a major environmental problem. Enzymes, particularly esterases, play an important role in the biodegradation of polyesters. These enzymes are usually only active on aliphatic polyesters, but a few have showed catalytic activity for semi-aromatic polyesters.
arxiv +1 more source
Towards Biochemical Filter with Sigmoidal Response to pH Changes: Buffered Biocatalytic Signal Transduction [PDF]
We realize a biochemical filtering process by introducing a buffer in a biocatalytic signal-transduction logic system based on the function of an enzyme, esterase. The input, ethyl butyrate, is converted into butyric acid-the output signal, which in turn is measured by the drop in the pH value. The developed approach offers a versatile "network element"
arxiv +1 more source
Regioselective chemo-enzymatic syntheses of ferulate conjugates as chromogenic substrates for feruloyl esterases [PDF]
Generally, carbohydrate-active enzymes are studied using chromogenic substrates that provide quick and easy color-based detection of enzyme-mediated hydrolysis. In the case of feruloyl esterases, commercially available chromogenic ferulate derivatives are both costly and limited in terms of their experimental application.
arxiv
Tunable Functionality and toxicity studies of Titanium Dioxide Nanotube Layers [PDF]
In this work, we have developed economic process to elaborate scalable titanium dioxide nanotube layers which show a tunable functionality. The titanium dioxide nanotube layers was prepared by electrochemical anodization of Ti foil in 0.4 wt% hydrofluoric acid solution.
arxiv +1 more source
Substrate-Enzyme Interaction in Pig Liver Esterase [PDF]
Force field and first principles molecular dynamics simulations on complexes of pig liver esterase (pig liver isoenzymes and a mutant) and selected substrates (1-phenyl-1-ethyl acetate, 1- phenyl-2-butylacetate, proline-{\beta}-naphthylamide and methyl butyrate) are presented.
arxiv
Beyond esterase-like activity of serum albumin. Histidine-(nitro)phenol radical formation in conversion cascade of p-nitrophenyl acetate and the role of infrared light [PDF]
Serum albumin, recognized mainly for its capacity to act as a carrier protein for many compounds, can also actively transform some organic molecules. As a starting point in this study we consider esterase-like activity of bovine serum albumin (BSA) toward p-nitrophenyl acetate (p-NPA).
arxiv
Micro-Bioreactor Mimicking a Cellular Environment [PDF]
We report that a cell-sized water droplet (CWD) with a diameter of several tens of microns can serve as a micro-biochemical reactor. Since the droplet inner surface is covered by a phospholipid layer, it provides an environment similar to that in living cells. The CWD is manipulated with laser tweezers and we monitor the time-development of biochemical
arxiv
Transcriptional Response of SK-N-AS Cells to Methamidophos [PDF]
Transcriptomics response of SK-N-AS cells to methamidophos (an acetylcholine esterase inhibitor) exposure was measured at 10 time points between 0.5 and 48 h. The data was analyzed using a combination of traditional statistical methods and novel machine learning algorithms for detecting anomalous behavior and infer causal relations between time ...
arxiv
THE COLORIMETRIC DETERMINATION OF LIPASE AND ESTERASE IN HUMAN SERUM 1 [PDF]
Arnold M. Seligman, Marvin M. Nachlas
openalex +1 more source
Action of esterase in the presence of organic solvents [PDF]
E. A. Sym
openalex +1 more source