Results 71 to 80 of about 132,817 (351)

Decoding Undesirable Inflammatory Responses of Nucleic Acid‐Delivering Lipid Nanoparticles

Advanced Science, EarlyView.
Lipid nanoparticles (LNPs) enable efficient nucleic acid delivery, but their immunogenicity is a double‐edged sword. This review explores LNP‐driven innate and adaptive immunity, covering lipid components, endosomal escape, and nucleic acid sensing.
Ruimin Hu, Yin Dou, Chenwen Li, Yuying Mu, Huiying Ouyang, Wenhao Shen, Jianxiang Zhang   +6 more
wiley   +1 more source

Novel Function of CtXyn5A from Acetivibrio thermocellus: Dual Arabinoxylanase and Feruloyl Esterase Activity Occur in the Same Active Site

, 2022
Uncharacterized side activities of enzymes can have significant negative effects on reaction products and yields. Hence, their identification and characterization is crucial for the development of successful reaction systems. Here, we report the presence
Savvina, Leontakianakou, Eva, Nordberg Karlsson, Patrick, Adlercreutz, Eva, Schmitz, Javier, Linares-Pastén   +4 more
core   +1 more source

Promiscuity‐Guided Enzyme Evolution via Substrate Multiplexed Screening

Angewandte Chemie, EarlyView.
Through historical context and recent examples, this Minireview summarizes the strengths, limitations, and practical considerations for the implementation of substrate multiplexed screening (SUMS) in enzyme evolution. ABSTRACT An enzyme's ability to react with diverse substrates while maintaining high yield and selectivity, termed “substrate ...
Holly A. Weilbaker, Meghan E. Campbell, Peyton M. Higgins, Andrew R. Buller   +3 more
wiley   +2 more sources

NONSPECIFIC ESTERASES IN MOSQUITOS [PDF]

Journal of Histochemistry & Cytochemistry, 1968
Esterase zymograms were prepared from 14 species and strains of Culicide mosquitoes by use of starch and polyacrylamide electrophoresis. The zymograms proved to be species- and strain-specific. Variations in the zymograms of individual mosquitos were demonstrated by use of the microgel technique. This technique is described. Sexual dimorphism was shown
T A, Freyvogel, R L, Hunter, E M, Smith
openaire   +2 more sources

Inflammation Unchecked: Concurrent Kawasaki Disease and Stevens‐Johnson Syndrome in an 18‐Month‐Old Child

Arthritis Care &Research, EarlyView.
Catherine Deffendall, Jodi Backalar, Elizabeth Acevedo Zapata, Adriana Della Porta, Lorin Bibb, Tyler Reese, Charlotte M. Brown   +6 more
wiley   +1 more source

Stage‐Specific H3K14 and H3K23 Succinylation Orchestrates Insect Metamorphosis and Oogenesis

Advanced Science, EarlyView.
Stage‐specific succinylation of histone H3 at lysine 14 and 23 differentially controls insect metamorphosis and oogenesis through distinct GPCR‐PKC signaling cascades, revealing an evolutionarily conserved epigenetic mechanism that coordinates key life‐history traits.
Yu‐Pu Jing, Lunjie Li, Libin Yang, Lin‐Jie Zhang, Qiang Yan, Ci Zhang, Zanrong Wen, Guangze Yu, Qian Li, Shutang Zhou   +9 more
wiley   +1 more source

Sporotrichum thermophile type C feruloyl esterase (StFaeC): Purification, characterization, and its use for phenolic acid (sugar) ester synthesis

, 2005
A feruloyl esterase (StFaeC) produced by Sporotrichum thermophile was purified to homogeneity. The native StFaeC was homodimer with a subunit of Mr 23,000 and pI 3.1. The enzyme activity was optimal at pH 6.0 and 55 °C.
Vafiadi, C, Christakopoulos, P, Vafiadi, C.,, Christakopoulos, Paul, Topakas, E, Topakas, E.,, Stamatis, H, Stamatis, H.,   +7 more
core   +1 more source

Thermostable Bioluminescent Intercalating Dyes for Real‐Time, Integrated Nucleic Acid Amplification and Detection

Angewandte Chemie, EarlyView.
We present a bioluminescent diagnostic platform that integrates DNA amplification and detection in a single step. The engineering of thermostable luciferase‐intercalating dye conjugates and controlled release of caged luciferin substrates enable real‐time, detection of attomolar concentrations of viral DNA within 30 min.
Yosta de Stigter, Harmen J. van der Veer, Sterre de Lignie, Robbert J. de Haas, Renko de Vries, Joost P. H. Schoeber, Anne J. M. Loonen, Adriaan J. C. van den Brule, Maarten Merkx   +8 more
wiley   +2 more sources

Synergistic Probe Combining Lysosome Anchoring and Tumor Defense System Targeting for Precise Tumor Visualization

Advanced Science, EarlyView.
A new generation of high‐precision fluorescent probes based on the lysosomal characteristics in tumor metabolic reprogramming and overexpressed tumor markers. The probe (CN‐D‐GGT) selectively label cancer cells in co‐culture systems and distinguish tumor lesions from inflamedand normal tissues in vivo, enabling precise tumor imaging.
Yang Shen, Jiawei Li, Ruixi Yi, Ling Shi, Wei Li, Sulai Liu, Lin Yuan   +6 more
wiley   +1 more source

Characterization of carboxylesterases involved in the insecticide resistance of Culex quinquefasciatus from the Caribbean and South America. [PDF]

The organophosphate resistance-associated elevated esterases Estα2, Estβ1 and Estβ2 were purified to homogeneity from larvae of the Cuban Habana strain.
Small, Graham John
core   +1 more source