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An ethidium ion selective electrode and its application to DNA-ethidium binding
Biochemical and Biophysical Research Communications, 1981Abstract A liquid membrane electrode has been made which is selective for ethidium ion. The membrane is formed in a capillary by a 3-nitro-o-xylene solution of an ethidium-tetraphenyl borate complex. The electrode emf (vs saturated KCl-calomel reference) has a linear dependence upon the logarithm of ethidium concentration from 2 μM to 0.5 mM.
T N, Blanton +2 more
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A synthesis of labeled ethidium bromide
Archives of Biochemistry and Biophysics, 1974Abstract A method is described for the synthesis of labeled ethidium (3,8-diamino-5-ethyl-6-phenyl phenanthridinium) bromide. Based on benzoic acid, the radioactive precursor used, the yield is 15% of a compound that is indistinguishable from authentic ethidium bromide in its absorption spectra (uv, visible, ir), Chromatographie behavior, and ...
R, De Nobrega Bastos, H R, Mahler
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Interaction of Ethidium Bromide with Heparin
International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, 1975The reactions of hydrated electrons produced during pulse radiolysis have been utilized to investigate the binding of ethidium bromide to heparin. Complexes of ethidium bromide and heparin can be dissociated with salt. Divalent cations are more effective than monovalent cations in this respect.
S, Chaudhuri +3 more
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Unfolding of nucleosomes by ethidium binding
Biochemistry, 1980We report spectroscopic, hydrodynamic, and biochemical studies on the complex of ethidium bromide with 140 base pair nucleosomal core particles. Fluorescence titration indicates a greater intrinsic affinity of ethidium for nucleosomes than for DNA, and fluorescence depolarization measurements imply increased immobilization of ethidium bound to ...
H M, Wu +3 more
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1975
Ethidium and propidium (Fig. 1) are phenanthridinium compounds which have attracted a great deal of interest in recent years because of their usefulness as tools for the experimental study of nucleic acids. Of the two ethidium is much the best known since it is readily available and inexpensive, being a well-established drug in widespread use for the ...
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Ethidium and propidium (Fig. 1) are phenanthridinium compounds which have attracted a great deal of interest in recent years because of their usefulness as tools for the experimental study of nucleic acids. Of the two ethidium is much the best known since it is readily available and inexpensive, being a well-established drug in widespread use for the ...
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Ethidium cooperativity in the formation of complexes with CpG
Biochemical and Biophysical Research Communications, 1986The formation of complexes between the self-complementary ribo-dinucleoside monophosphate CpG and ethidium ion is observed by use of an ethidium ion selective electrode. The ratio of total CpG to total ethidium was varied from 50:1 to .4:1, with CpG concentrations ranging from 0.2 to 1.1 mM.
M, Day, C G, Trowbridge
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Staining of Nuclei in Fungi by Ethidium Bromide
Biotechnic & Histochemistry, 1991Ethidium bromide, (0.1% solution in ethanol-water, 1:3, v/v) was used to stain nuclei in mycelia and spores of different fungi. Nuclei looked bright brick red under green excitation. This method is very efficient, specific, reproducible and cost-effective.
U S, Singh, J, Kumar
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2000
Abstract Ethidium bromide (EB), a phenanthridinium dye, is used as an antitrypanosomal drug in veterinary medicine and, in the research setting, as a membrane-permeant cell marker (11), a DNA stain, and an experimental arthropod and mammalian neurotoxin and cytotoxin. EB forms bitter-tasting, dark-red crystals with a
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Abstract Ethidium bromide (EB), a phenanthridinium dye, is used as an antitrypanosomal drug in veterinary medicine and, in the research setting, as a membrane-permeant cell marker (11), a DNA stain, and an experimental arthropod and mammalian neurotoxin and cytotoxin. EB forms bitter-tasting, dark-red crystals with a
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Mitochondrial membranes and mutagenesis by ethidium bromide
Journal of Supramolecular Structure, 1972AbstractThe conversion of wild type (ρ+) to cytoplasmic petites (ρ−) in Saccharomyces cerevisiae, à mutation in mitochondrial DNA, can be brought about with high efficiency by low concentrations of ethidium bromide (EB). The rate and extent of mutagenesis and its expression can be influenced, and even reversed, by a number of genetic lesions, agents or
H R, Mahler, P S, Perlman
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Destruction of Ethidium Bromide in Solution by Ozonolysis
Biological Chemistry Hoppe-Seyler, 1988Ethidium bromide can be rapidly destroyed in aqueous solutions or in isoamyl alcohol by ozonolysis in the presence of H2O2 to give a mixture of organic acids. In a variety of buffers commonly used in recombinant DNA technology destruction of ethidium bromide was more than 99.9%. The yellow reaction mixture after ozonolysis was shown to be nonmutagenic.
R, Zocher +3 more
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