Results 71 to 80 of about 146,802 (237)

Fluorometric determination of ethidium bromide efflux kinetics in Escherichia coli

Journal of Biological Engineering, 2009
Background Efflux pump activity has been associated with multidrug resistance phenotypes in bacteria, compromising the effectiveness of antimicrobial therapy.
Monteiro Gabriel A, de Carvalho Carla CCR, Fernandes Pedro, Martins Marta, Couto Isabel, Rodrigues Liliana, Paixão Laura, Sansonetty Filipe, Amaral Leonard, Viveiros Miguel   +9 more
doaj   +1 more source

Significance of Fluorescent Spectroscopy in Screening Oral Potentially Malignant Disorders and Oral Cancer by Characterization of Salivary DNA Using Ethidium Bromide-A Comparative Study. [PDF]

South Asian J Cancer, 2023
Jayachnadran S, Prakasarao A, Ramamoorthy S, Manoharan Y.   +3 more
europepmc   +1 more source

KU80 suppresses endonuclease G activity to preserve genomic integrity

The FEBS Journal, EarlyView.
Under normal conditions, EndoG remains restricted to mitochondria and the genome remains intact. When KU80 is absent, EndoG translocates into the nucleus, where it promotes DNA fragmentation and genomic instability. Thus, this work highlights the importance of KU80 in tightly controlling EndoG localization to preserve genome stability.
Jargalan Batsaikhan, Kwang‐Hyun Park, Hae Ryung Chang, Eunyoung Jung, Myung‐Jin Kim, Jeeyoon Kang, Hyunjo Shim, Eui‐Jeon Woo, Yonghwan Kim   +8 more
wiley   +1 more source

A cryptic targeting signal creates a mitochondrial FEN1 isoform with tailed R-Loop binding properties.

PLoS ONE, 2013
A growing number of DNA transacting proteins is found in the nucleus and in mitochondria, including the DNA repair and replication protein Flap endonuclease 1, FEN1. Here we show a truncated FEN1 isoform is generated by alternative translation initiation,
Lawrence Kazak, Aurelio Reyes, Jiuya He, Stuart R Wood, Gloria Brea-Calvo, Torgeir T Holen, Ian J Holt   +6 more
doaj   +1 more source

Forskolin, an Adenylcyclase/cAMP/CREB Signaling Activator Restoring Myelin-Associated Oligodendrocyte Destruction in Experimental Ethidium Bromide Model of Multiple Sclerosis. [PDF]

Cells, 2022
Kapoor T, Mehan S, Suri M, Sharma N, Kumar N, Narula AS, Alshammari A, Alasmari AF, Alharbi M, Assiri MA, Kalfin R.   +10 more
europepmc   +1 more source

Intercalation of ethidium bromide into a triple-stranded oligonucleotide

Nucleic Acids Research, 1991
We have examined the ability of a cationic planar chromophore, ethidium bromide, to intercalate into a short, defined triple helix. Using UV absorption, fluorescence spectroscopy and a gel retardation assay we demonstrate that ethidium bromide is able to bind to a triple helix with a lower affinity than to the corresponding duplex. Energy transfer from
J L, Mergny, D, Collier, M, Rougée, T, Montenay-Garestier, C, Hélène   +4 more
openaire   +3 more sources

A distinct gene expression mechanism for d‐amino acid utilization in hyperthermophilic archaeon Pyrococcus horikoshii

The FEBS Journal, EarlyView.
The genes PH0137, PH0138, and PH0140 form a cluster in the genome of Pyrococcus horikoshii. The PH0140 gene encodes a transcription factor identified as a d‐amino acid‐responsive regulatory protein (DARP). In feast mode, l‐Ile‐bound DARP binds to the promoter and represses transcription of PH0137 and PH0138. In famine mode, DARP binds to d‐allo‐Ile and
Ryushi Kawakami, Tomoki Kawase, Taira Uehara, Haruhiko Sakuraba, Tomohiko Matsuzawa, Junji Hayashi, Toshihisa Ohshima   +6 more
wiley   +1 more source

Bacillus cereus efflux protein BC3310 - a multidrug transporter of the unknown major facilitator family, UMF-2

Frontiers in Microbiology, 2015
Phylogenetic classification divides the major facilitator superfamily (MFS) into 82 families, including 25 families that are comprised of transporters with no characterized functions.
Jasmin K Kroeger, Karl eHassan, Aniko eVörös, Roger eSimm, Roger eSimm, Massoud eSaidijam, Massoud eSaidijam, Kim E Bettaney, Andreas eBechthold, Ian T Paulsen, Peter eHenderson, Anne-Brit eKolstø   +11 more
doaj   +1 more source

Ethidium bromide interactions with DNA: an exploration of a classic DNA-ligand complex with unbiased molecular dynamics simulations. [PDF]

Nucleic Acids Res, 2021
Galindo-Murillo R, Cheatham TE.
europepmc   +1 more source

Astrocytic connexin43 phosphorylation contributes to seizure susceptibility after mild traumatic brain injury

The FEBS Journal, EarlyView.
Astrocytic gap junctions formed by connexin43 (Cx43) help maintain brain homeostasis. After mild traumatic brain injury (TBI), total cortical Cx43 increases and redistributes toward a soluble, non‐junctional pool with elevated hemichannel activity and increased phosphorylation at serine 368 (pCx43S368).
Carmen Muñoz‐Ballester, Owen Leitzel, Samantha Golf, Chelsea M. Phillips, Michael J. Zeitz, Rahul Pandit, Elizabeth Wash, Jenna V. Donohue, Elizabeth A. Istre, James W. Smyth, Samy Lamouille, Stefanie Robel   +11 more
wiley   +1 more source