Results 211 to 220 of about 153,070 (262)

Mitochondrial membranes and mutagenesis by ethidium bromide

Journal of Supramolecular Structure, 1972
AbstractThe conversion of wild type (ρ+) to cytoplasmic petites (ρ−) in Saccharomyces cerevisiae, à mutation in mitochondrial DNA, can be brought about with high efficiency by low concentrations of ethidium bromide (EB). The rate and extent of mutagenesis and its expression can be influenced, and even reversed, by a number of genetic lesions, agents or
H R, Mahler, P S, Perlman
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Destruction of Ethidium Bromide in Solution by Ozonolysis

Biological Chemistry Hoppe-Seyler, 1988
Ethidium bromide can be rapidly destroyed in aqueous solutions or in isoamyl alcohol by ozonolysis in the presence of H2O2 to give a mixture of organic acids. In a variety of buffers commonly used in recombinant DNA technology destruction of ethidium bromide was more than 99.9%. The yellow reaction mixture after ozonolysis was shown to be nonmutagenic.
R, Zocher, A, Billich, U, Keller, P, Messner   +3 more
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An evaluation of the ethidium bromide microcytotoxicity test

Journal of Immunological Methods, 1974
Abstract An ethidium bromide fluorescence technique for the demonstration of lymphocytotoxic antibodies is described and evaluated. This fluorescence technique offers the advantages of speed, simplicity, high reproducibility and sensitivity.
C, Darke, E, Gay
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Staining of Nuclei in Fungi by Ethidium Bromide

Biotechnic & Histochemistry, 1991
Ethidium bromide, (0.1% solution in ethanol-water, 1:3, v/v) was used to stain nuclei in mycelia and spores of different fungi. Nuclei looked bright brick red under green excitation. This method is very efficient, specific, reproducible and cost-effective.
U S, Singh, J, Kumar
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Yeast mutants resistant to ethidium bromide

Molecular and General Genetics MGG, 1973
Yeast mutants resistant to ethidium bromide have been isolated among sensitive grande cells (ϱ+) for their ability to grow on glycerol in the presence of the dye. Mutant cells are also resistant to acriflavin and do not yield petites (ϱ-) when grown on galactose with the mutagen.
M, Gouhier, J C, Mounolou
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Renaturation of DNA in the presence of ethidium bromide

Biopolymers, 1972
AbstractThe rate of renaturation of T2 DNA has been studied as a fuction of ethidium bound per nucleotide of denatured DNA. The Binding constants and number of binding sites for ethidium have been determined by spectral titration for denatured DNA at 55, 65, and 75°C and for native DNA at 65°C in 0.4M Na+.
J R, Hutton, J G, Wetmur
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Ethidium Bromide

2000
Abstract Ethidium bromide (EB), a phenanthridinium dye, is used as an antitrypanosomal drug in veterinary medicine and, in the research setting, as a membrane-permeant cell marker (11), a DNA stain, and an experimental arthropod and mammalian neurotoxin and cytotoxin. EB forms bitter-tasting, dark-red crystals with a
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Binding of ethidium bromide to fractionated chromatin

Experimental Cell Research, 1976
Abstract The binding of ethidium bromide (EB) to different chromatin preparations was tested. Scatchard plots showed that the slowly sedimenting fraction of sheared chromatin is enriched in dye-binding sites. Limited nuclease digestion of rat liver nuclei, which has been shown to preserve the subunit structure of chromatin, reduces the number of ...
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Crystal structure of ethidium bromide

Journal of Crystal and Molecular Structure, 1971
Crystals of ethidium bromide (2,7-diamino-9-phenyl-10-ethylphenanthridinium bromide) monohydrate are monoclinic,a = 9·577,b = 10·698,c = 20·242 A, β = 106·34 °,Z = 4, space groupP21/c. The structure was determined with CuKα. diffractometer data by direct methods, and was refined by full-matrix least-squares methods toR = 0·058 for 2151 observed ...
E. Subramanian, James Trotter, Charles E. Bugg   +2 more
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Decontamination of Ethidium Bromide Spills

Applied Industrial Hygiene, 1989
Abstract Samples of stainless steel, Formica, glass, and vinyl floor tile and the filters of transilluminators were contaminated with ethidium bromide and cleaned either by wiping once with a paper towel soaked in an aqueous solution of sodium nitrite and hypophosphorous acid followed by five wipes with wet towels or by wiping six times with only wet ...
George Lunn, Eric B. Sansone
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