Results 181 to 190 of about 146,802 (237)
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Inhibition of yeast sporulation by ethidium bromide
Molecular Genetics and Genomics, 1978Ethidium bromide blocks ascus formation in the yeast Saccharomyces cerevisiae. This may mean that the presence of the mitochondrial genome is required for sporulation in this organism.
B D Hall +2 more
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Interaction of Ethidium Bromide with Heparin
International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, 1975The reactions of hydrated electrons produced during pulse radiolysis have been utilized to investigate the binding of ethidium bromide to heparin. Complexes of ethidium bromide and heparin can be dissociated with salt. Divalent cations are more effective than monovalent cations in this respect.
S, Chaudhuri +3 more
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A synthesis of labeled ethidium bromide
Archives of Biochemistry and Biophysics, 1974Abstract A method is described for the synthesis of labeled ethidium (3,8-diamino-5-ethyl-6-phenyl phenanthridinium) bromide. Based on benzoic acid, the radioactive precursor used, the yield is 15% of a compound that is indistinguishable from authentic ethidium bromide in its absorption spectra (uv, visible, ir), Chromatographie behavior, and ...
R, De Nobrega Bastos, H R, Mahler
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Mitochondrial membranes and mutagenesis by ethidium bromide
Journal of Supramolecular Structure, 1972AbstractThe conversion of wild type (ρ+) to cytoplasmic petites (ρ−) in Saccharomyces cerevisiae, à mutation in mitochondrial DNA, can be brought about with high efficiency by low concentrations of ethidium bromide (EB). The rate and extent of mutagenesis and its expression can be influenced, and even reversed, by a number of genetic lesions, agents or
H R, Mahler, P S, Perlman
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Destruction of Ethidium Bromide in Solution by Ozonolysis
Biological Chemistry Hoppe-Seyler, 1988Ethidium bromide can be rapidly destroyed in aqueous solutions or in isoamyl alcohol by ozonolysis in the presence of H2O2 to give a mixture of organic acids. In a variety of buffers commonly used in recombinant DNA technology destruction of ethidium bromide was more than 99.9%. The yellow reaction mixture after ozonolysis was shown to be nonmutagenic.
R, Zocher +3 more
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An evaluation of the ethidium bromide microcytotoxicity test
Journal of Immunological Methods, 1974Abstract An ethidium bromide fluorescence technique for the demonstration of lymphocytotoxic antibodies is described and evaluated. This fluorescence technique offers the advantages of speed, simplicity, high reproducibility and sensitivity.
C, Darke, E, Gay
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Staining of Nuclei in Fungi by Ethidium Bromide
Biotechnic & Histochemistry, 1991Ethidium bromide, (0.1% solution in ethanol-water, 1:3, v/v) was used to stain nuclei in mycelia and spores of different fungi. Nuclei looked bright brick red under green excitation. This method is very efficient, specific, reproducible and cost-effective.
U S, Singh, J, Kumar
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Yeast mutants resistant to ethidium bromide
Molecular and General Genetics MGG, 1973Yeast mutants resistant to ethidium bromide have been isolated among sensitive grande cells (ϱ+) for their ability to grow on glycerol in the presence of the dye. Mutant cells are also resistant to acriflavin and do not yield petites (ϱ-) when grown on galactose with the mutagen.
M, Gouhier, J C, Mounolou
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Renaturation of DNA in the presence of ethidium bromide
Biopolymers, 1972AbstractThe rate of renaturation of T2 DNA has been studied as a fuction of ethidium bound per nucleotide of denatured DNA. The Binding constants and number of binding sites for ethidium have been determined by spectral titration for denatured DNA at 55, 65, and 75°C and for native DNA at 65°C in 0.4M Na+.
J R, Hutton, J G, Wetmur
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