Results 51 to 60 of about 146,802 (237)

Comparison of techniques for DNA extraction and agarose gel staining of DNA fragments using samples of Cryptosporidium

open access: yesVeterinární Medicína, 2013
Differentiating between the Cryptosporidium species and their subtypes using only microscopy is impossible. Therefore, molecular tools are indispensable for accurate species and subtype diagnosis.
M.C.M. Couto   +3 more
doaj   +1 more source

Evaluation of DNA/Chromosome Integrity and Cell Death in Human Metabolically Noncompetent and Competent Cells Exposed to N′‐(3,5‐Difluorobenzylidene)Pyridine‐4‐Carbohydrazide

open access: yesJournal of Applied Toxicology, EarlyView.
ABSTRACT The N‐acylhydrazone scaffold is recognized as a privileged structure for the design of bioactive substances with increasing applications in medicinal chemistry research. Ensuring the safety of newly developed molecules is a critical step for both human health and environmental protection. Accordingly, this study aimed to evaluate the cytotoxic
Larissa Ribeiro Canuto Santos   +4 more
wiley   +1 more source

Neuroprotective Effects of Myricitrin on Cognitive Deficits in Hippacampal Demyelinated Rats

open access: yesJournal of Mazandaran University of Medical Sciences, 2019
Background and purpose: Multiple sclerosis (MS) as an autoimmune disease is the most common demyelinating inflammatory disease in young people that affects the central nervous system.
Fatemeh SargolzaeeJavan   +2 more
doaj  

The Impact of Genetic Polymorphisms on Genotoxicity (DNA Damage) Among Children Exposed to Environmental Mutagens: A Systematic Review

open access: yesJournal of Applied Toxicology, EarlyView.
ABSTRACT Children, as a particularly vulnerable group, are more susceptible to these mutagens due to their developing immune systems, higher physiological vulnerability, and increased exposure through behaviors like outdoor play and hand‐to‐mouth activities. This review aims to investigate the relationship between these environmental exposures, genetic
Thiago Guedes Pinto   +7 more
wiley   +1 more source

Synthetic polymer nanocarrier enhances dsRNA stability but not RNAi efficacy in Brassicogethes aeneus

open access: yesPest Management Science, EarlyView.
Experimental setup for dsRNA feeding in Brassicogethes aeneus to evaluate the effect of a synthetic polymer nanocarrier on RNAi efficiency. The experiment assessed whether the nanocarrier enhances RNAi after oral dsRNA delivery. (a) Stability of dsRNA–nanocarrier complexes was tested in simulated gut conditions. (b) Gene expression of αCOP and dre4 was
Triin Kallavus   +13 more
wiley   +1 more source

2D van der Waals System Integrated with Targeting Radio‐Diagnostic and Imaging Functions

open access: yesSmall, EarlyView.
Layered double hydroxide (LDH) nanoplates were engineered for tumor‐targeted imaging and therapy. By exploiting folate receptor (FR) expression levels, the system achieves selective tumor accumulation and high‐resolution dual PET/fluorescence (PET/FL) imaging.
Sairan Eom   +6 more
wiley   +1 more source

Investigation of Gel Electrophoresis Stains

open access: yesAl-SalamJournal for Medical Science
Gel electrophoresis is essential in molecular biology and biochemistry for separating and analyzing DNA, RNA, and proteins. UV sensitivity and compatibility have led to the use of ethidium bromide stains.
ibrahim ramzi hadi   +4 more
doaj   +1 more source

Mutagenic Improvement of Xylanase Production from Xylanolytic Bacteria and its Phylogenetic Analysis

open access: yesMicrobiology Indonesia, 2013
This study was conducted to obtain xylanolytic mutants that have higher xylanase activity than their wildtype counterparts. A mutant with the best xylanolytic activity was selected and identified based on its 16S rRNA sequence.
CHUSNUL HANIM   +3 more
doaj   +1 more source

Cytogenotoxic and Molecular Assessment of Pyrethrins on Non‐Target Allium cepa Roots

open access: yesEnvironmental Toxicology, EarlyView.
ABSTRACT The pyrethrins, which form a part of naturally occurring insecticidal chemicals derived from chrysanthemum flowers, not only provide rapid knockdown of a broad spectrum of insect pests but are also widely used in integrated pest management due to a generally favorable safety profile, largely attributed to low mammalian toxicity.
Yudum Yeltekin Uğur   +2 more
wiley   +1 more source

Protocol for high-sensitivity/long linear-range spectrofluorimetric DNA quantification using ethidium bromide

open access: yesBioTechniques, 2007
Ethidium bromide (EtBr) is the most widely used fluorescent dye in nucleic acid gel electrophoresis since decades. However, it has been essentially forgotten in DNA quantification by spectrofluorimetry.
Veronica Bonasera   +2 more
doaj   +1 more source

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