Results 51 to 60 of about 1,148 (171)

Comparison of techniques for DNA extraction and agarose gel staining of DNA fragments using samples of Cryptosporidium

Veterinární Medicína, 2013
Differentiating between the Cryptosporidium species and their subtypes using only microscopy is impossible. Therefore, molecular tools are indispensable for accurate species and subtype diagnosis.
M.C.M. Couto, A.P. Sudre, M.F. Lima, T.C.B. Bomfim   +3 more
doaj   +1 more source

Synthetic polymer nanocarrier enhances dsRNA stability but not RNAi efficacy in Brassicogethes aeneus

Pest Management Science, EarlyView.
Experimental setup for dsRNA feeding in Brassicogethes aeneus to evaluate the effect of a synthetic polymer nanocarrier on RNAi efficiency. The experiment assessed whether the nanocarrier enhances RNAi after oral dsRNA delivery. (a) Stability of dsRNA–nanocarrier complexes was tested in simulated gut conditions. (b) Gene expression of αCOP and dre4 was
Triin Kallavus, Liina Soonvald, Iris Callewaert, Ana Isabel Silva, Ericmar Ávila Dos Santos, Silva Vilumets, Riina Kaasik, Sven Vereecken, Lenny Van Daele, Peter Dubruel, Jonathan Willow, Clauvis Nji Tizi Taning, Kristof De Schutter, Eve Veromann   +13 more
wiley   +1 more source

Neuroprotective Effects of Myricitrin on Cognitive Deficits in Hippacampal Demyelinated Rats

Journal of Mazandaran University of Medical Sciences, 2019
Background and purpose: Multiple sclerosis (MS) as an autoimmune disease is the most common demyelinating inflammatory disease in young people that affects the central nervous system.
Fatemeh SargolzaeeJavan, Mohammad Ali Mirshekar, Mohammadreza Shahraki   +2 more
doaj  

2D van der Waals System Integrated with Targeting Radio‐Diagnostic and Imaging Functions

Small, EarlyView.
Layered double hydroxide (LDH) nanoplates were engineered for tumor‐targeted imaging and therapy. By exploiting folate receptor (FR) expression levels, the system achieves selective tumor accumulation and high‐resolution dual PET/fluorescence (PET/FL) imaging.
Sairan Eom, Jae Hun Ahn, Jung Yung Kim, Kyo Chul Lee, Yong Jin Lee, Sanoj Rejinold N, Jin‐Ho Choy   +6 more
wiley   +1 more source

Endocytosis‐independent cytosolic entry of messenger RNA via fluorous bilayer zippering attenuating Toll‐like receptor signaling and enables ischemic tissue salvage

Smart Molecules, EarlyView.
The present study establishes a paradigm shift in synthetic mRNA delivery through the convergence of three orthogonal chemistries—perfluoro‐acylation, bio‐orthogonal click cross‐linking, and disulfide reduction—within a single, modular polymer architecture. Abstract A fundamental constraint of conventional messenger RNA (mRNA) delivery systems is their
Yue Wang, Haitao Xie, Guoqing Xiang, Yanhua Li, Changgui Tong, Hongyan Cui, Qixian Chen, Haidong Li, Yan Zhao   +8 more
wiley   +1 more source

Cytogenotoxic and Molecular Assessment of Pyrethrins on Non‐Target Allium cepa Roots

Environmental Toxicology, EarlyView.
ABSTRACT The pyrethrins, which form a part of naturally occurring insecticidal chemicals derived from chrysanthemum flowers, not only provide rapid knockdown of a broad spectrum of insect pests but are also widely used in integrated pest management due to a generally favorable safety profile, largely attributed to low mammalian toxicity.
Yudum Yeltekin Uğur, Rahsan Ilikci‐Sagkan, Recep Liman   +2 more
wiley   +1 more source

Investigation of Gel Electrophoresis Stains

Al-SalamJournal for Medical Science
Gel electrophoresis is essential in molecular biology and biochemistry for separating and analyzing DNA, RNA, and proteins. UV sensitivity and compatibility have led to the use of ethidium bromide stains.
ibrahim ramzi hadi, Dilara Bozdogan, Ali Ahmed Kamal Khedher Agha, Ruaa Hussein Ali, Safa Al-Shattawi   +4 more
doaj   +1 more source

Mutagenic Improvement of Xylanase Production from Xylanolytic Bacteria and its Phylogenetic Analysis

Microbiology Indonesia, 2013
This study was conducted to obtain xylanolytic mutants that have higher xylanase activity than their wildtype counterparts. A mutant with the best xylanolytic activity was selected and identified based on its 16S rRNA sequence.
CHUSNUL HANIM, LIES MIRA YUSIATI, MUHAMMAD NUR CAHYANTO, ALI WIBOWO   +3 more
doaj   +1 more source

Prevalence and Genomic Characterization of mcr‐Positive Enterobacteriaceae in Retail Meat in Thailand Following the Colistin Ban

Microbiology and Immunology, EarlyView.
ABSTRACT This study aimed to investigate the prevalence and characteristics of mcr‐positive Enterobacteriaceae in retail meat in Thailand following the national ban on prophylactic colistin use in food producing animals. A total of 152 meat samples (103 chicken and 49 pork) were collected from supermarkets and open markets between July and September ...
Ahmed Abououf, Sharda Prasad Awasthi, Asmaa M. Elbastawesy, Noritoshi Hatanaka, Atsushi Hinenoya, Srinuan Somroop, Yukihiro Kaneko, Shinji Yamasaki   +7 more
wiley   +1 more source

Ameliorating the Effects of Trypanosoma Evansi Infection in Wistar Rats Using Selected Nutraceuticals, Ethidium Bromide and Ethidium Chloride

UMYU Scientifica Journal
African Trypanosomiasis is a disease that affects humans and animals, caused by trypanosomes in sub-Saharan Africa, despite decades of vector control and treatments. The disease remains endemic and an impediment to socio-economic development.
Yahaya Shuaibu, Abdulfatai, K., Dadah, A. J.   +2 more
doaj   +1 more source