Results 41 to 50 of about 146,910 (158)

DNA extraction from formalin-fixed tissue: new light from the deep sea

open access: yesScientia Marina, 2010
DNA samples were extracted from ethanol and formalin-fixed decapod crustacean tissue using a new method based on Tetramethylsilane (TMS)-Chelex. It is shown that neither an indigestible matrix of cross-linked protein nor soluble PCR inhibitors impede PCR
Ferran Palero   +5 more
doaj   +1 more source

Zaleya decandra exerts anti-cancer activity in HCT-116 cancer cells by impairing mitochondrial activity

open access: yesBangladesh Journal of Pharmacology, 2019
An attempt has been made to investigate the role of Zaleya decandra on the survival of HCT colon cancer cells in vitro. The cell viability and apoptosis were assessed using MTT assay and acridine orange/ethidium bromide staining, respectively ...
Malarvizhi Deivasigamani   +4 more
doaj   +1 more source

GC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps

open access: yesAntibiotics, 2020
Considering the evidence that essential oils, as well as safrole, could modulate bacterial growth in different resistant strains, this study aims to characterize the phytochemical profile and evaluate the antibacterial and antibiotic-modulating ...
Ray S. Almeida   +14 more
doaj   +1 more source

Comparison of techniques for DNA extraction and agarose gel staining of DNA fragments using samples of Cryptosporidium

open access: yesVeterinární Medicína, 2013
Differentiating between the Cryptosporidium species and their subtypes using only microscopy is impossible. Therefore, molecular tools are indispensable for accurate species and subtype diagnosis.
M.C.M. Couto   +3 more
doaj   +1 more source

Neuroprotective Effects of Myricitrin on Cognitive Deficits in Hippacampal Demyelinated Rats

open access: yesJournal of Mazandaran University of Medical Sciences, 2019
Background and purpose: Multiple sclerosis (MS) as an autoimmune disease is the most common demyelinating inflammatory disease in young people that affects the central nervous system.
Fatemeh SargolzaeeJavan   +2 more
doaj  

Investigation of Gel Electrophoresis Stains

open access: yesAl-SalamJournal for Medical Science
Gel electrophoresis is essential in molecular biology and biochemistry for separating and analyzing DNA, RNA, and proteins. UV sensitivity and compatibility have led to the use of ethidium bromide stains.
ibrahim ramzi hadi   +4 more
doaj   +1 more source

Mutagenic Improvement of Xylanase Production from Xylanolytic Bacteria and its Phylogenetic Analysis

open access: yesMicrobiology Indonesia, 2013
This study was conducted to obtain xylanolytic mutants that have higher xylanase activity than their wildtype counterparts. A mutant with the best xylanolytic activity was selected and identified based on its 16S rRNA sequence.
CHUSNUL HANIM   +3 more
doaj   +1 more source

Protocol for high-sensitivity/long linear-range spectrofluorimetric DNA quantification using ethidium bromide

open access: yesBioTechniques, 2007
Ethidium bromide (EtBr) is the most widely used fluorescent dye in nucleic acid gel electrophoresis since decades. However, it has been essentially forgotten in DNA quantification by spectrofluorimetry.
Veronica Bonasera   +2 more
doaj   +1 more source

Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison

open access: yesGenetics and Molecular Biology, 2009
The present study applied the comet assay to erythrocytes of Oreochromis niloticus with the aim of improving protocols to detect DNA damage in these cells, by using two distinct pHs (pH = 12.1 and pH > 13) and evaluating whether there is a correspondence
Cintya A. Christofoletti   +2 more
doaj  

Home - About - Disclaimer - Privacy