Results 121 to 130 of about 1,148 (171)
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An evaluation of the ethidium bromide microcytotoxicity test
Journal of Immunological Methods, 1974Abstract An ethidium bromide fluorescence technique for the demonstration of lymphocytotoxic antibodies is described and evaluated. This fluorescence technique offers the advantages of speed, simplicity, high reproducibility and sensitivity.
C, Darke, E, Gay
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Staining of Nuclei in Fungi by Ethidium Bromide
Biotechnic & Histochemistry, 1991Ethidium bromide, (0.1% solution in ethanol-water, 1:3, v/v) was used to stain nuclei in mycelia and spores of different fungi. Nuclei looked bright brick red under green excitation. This method is very efficient, specific, reproducible and cost-effective.
U S, Singh, J, Kumar
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Yeast mutants resistant to ethidium bromide
Molecular and General Genetics MGG, 1973Yeast mutants resistant to ethidium bromide have been isolated among sensitive grande cells (ϱ+) for their ability to grow on glycerol in the presence of the dye. Mutant cells are also resistant to acriflavin and do not yield petites (ϱ-) when grown on galactose with the mutagen.
M, Gouhier, J C, Mounolou
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Renaturation of DNA in the presence of ethidium bromide
Biopolymers, 1972AbstractThe rate of renaturation of T2 DNA has been studied as a fuction of ethidium bound per nucleotide of denatured DNA. The Binding constants and number of binding sites for ethidium have been determined by spectral titration for denatured DNA at 55, 65, and 75°C and for native DNA at 65°C in 0.4M Na+.
J R, Hutton, J G, Wetmur
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2000
Abstract Ethidium bromide (EB), a phenanthridinium dye, is used as an antitrypanosomal drug in veterinary medicine and, in the research setting, as a membrane-permeant cell marker (11), a DNA stain, and an experimental arthropod and mammalian neurotoxin and cytotoxin. EB forms bitter-tasting, dark-red crystals with a
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Abstract Ethidium bromide (EB), a phenanthridinium dye, is used as an antitrypanosomal drug in veterinary medicine and, in the research setting, as a membrane-permeant cell marker (11), a DNA stain, and an experimental arthropod and mammalian neurotoxin and cytotoxin. EB forms bitter-tasting, dark-red crystals with a
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Beiträge zur Pathologie, 1976
Chick embryo fibroblasts cultivated in vitro were treated with ethidium bromide (E.B.) or with DNA-E.B. complex (DNA-E.B.). E.B. (5 mug/ml) provokes morphological alterations and cell death, inhibits DNA synthesis and mitotic activity. DNA-E.B. (E.B. 5 mug/ml) is less toxic to the fibroblasts as far as cell structure, DNA synthesis and mitotic activity
E, Heinen, R, Bassleer, C M, Calberg
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Chick embryo fibroblasts cultivated in vitro were treated with ethidium bromide (E.B.) or with DNA-E.B. complex (DNA-E.B.). E.B. (5 mug/ml) provokes morphological alterations and cell death, inhibits DNA synthesis and mitotic activity. DNA-E.B. (E.B. 5 mug/ml) is less toxic to the fibroblasts as far as cell structure, DNA synthesis and mitotic activity
E, Heinen, R, Bassleer, C M, Calberg
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Binding of ethidium bromide to fractionated chromatin
Experimental Cell Research, 1976Abstract The binding of ethidium bromide (EB) to different chromatin preparations was tested. Scatchard plots showed that the slowly sedimenting fraction of sheared chromatin is enriched in dye-binding sites. Limited nuclease digestion of rat liver nuclei, which has been shown to preserve the subunit structure of chromatin, reduces the number of ...
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Crystal structure of ethidium bromide
Journal of Crystal and Molecular Structure, 1971Crystals of ethidium bromide (2,7-diamino-9-phenyl-10-ethylphenanthridinium bromide) monohydrate are monoclinic,a = 9·577,b = 10·698,c = 20·242 A, β = 106·34 °,Z = 4, space groupP21/c. The structure was determined with CuKα. diffractometer data by direct methods, and was refined by full-matrix least-squares methods toR = 0·058 for 2151 observed ...
E. Subramanian +2 more
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Decontamination of Ethidium Bromide Spills
Applied Industrial Hygiene, 1989Abstract Samples of stainless steel, Formica, glass, and vinyl floor tile and the filters of transilluminators were contaminated with ethidium bromide and cleaned either by wiping once with a paper towel soaked in an aqueous solution of sodium nitrite and hypophosphorous acid followed by five wipes with wet towels or by wiping six times with only wet ...
George Lunn, Eric B. Sansone
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Metabolism of ethidium bromide in rats.
Drug Metabolism and Disposition, 1981The biliary excretion products recovered after iv administration of ethidium bromide to rats were found to consist of: unchanged drug, 8-acetylethidium, and an 8-acetylated hydroxylated metabolite. Proofs of structure were obtained by field desorption mass spectroscopy and 1H NMR, which indicated that the latter metabolite is probably substituted at ...
C, Fraire, P, Lecointe, C, Paoletti
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