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Crystal structure of ethidium bromide

Journal of Crystal and Molecular Structure, 1971
Crystals of ethidium bromide (2,7-diamino-9-phenyl-10-ethylphenanthridinium bromide) monohydrate are monoclinic,a = 9·577,b = 10·698,c = 20·242 A, β = 106·34 °,Z = 4, space groupP21/c. The structure was determined with CuKα. diffractometer data by direct methods, and was refined by full-matrix least-squares methods toR = 0·058 for 2151 observed ...
E. Subramanian   +2 more
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Decontamination of Ethidium Bromide Spills

Applied Industrial Hygiene, 1989
Abstract Samples of stainless steel, Formica, glass, and vinyl floor tile and the filters of transilluminators were contaminated with ethidium bromide and cleaned either by wiping once with a paper towel soaked in an aqueous solution of sodium nitrite and hypophosphorous acid followed by five wipes with wet towels or by wiping six times with only wet ...
George Lunn, Eric B. Sansone
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Mitochondrial membranes and mutagenesis by ethidium bromide

Journal of Supramolecular Structure, 1972
AbstractThe conversion of wild type (ρ+) to cytoplasmic petites (ρ−) in Saccharomyces cerevisiae, à mutation in mitochondrial DNA, can be brought about with high efficiency by low concentrations of ethidium bromide (EB). The rate and extent of mutagenesis and its expression can be influenced, and even reversed, by a number of genetic lesions, agents or
H R, Mahler, P S, Perlman
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Inhibition of yeast sporulation by ethidium bromide

Molecular and General Genetics MGG, 1978
Ethidium bromide blocks ascus formation in the yeast Saccharomyces cerevisiae. This may mean that the presence of the mitochondrial genome is required for sporulation in this organism.
M C, Newlon, B D, Hall
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Ethidium bromide enhancement of frameshift mutagenesis caused by photoactivatable ethidium analogs

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 1979
Ethidium azide analogs (3-amino-8-azido-ethidium monoazide and ethidium diazide) have been developed as photosensitive probes in order to analyze directly the reversible in vivo interactions of ethidium bromide. Our preliminary observations [11], relating the mutagenic potential of the monoazide analog of ethidium, have been extended and refined, using
L W, Yielding   +3 more
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Metabolism of ethidium bromide in rats.

Drug Metabolism and Disposition, 1981
The biliary excretion products recovered after iv administration of ethidium bromide to rats were found to consist of: unchanged drug, 8-acetylethidium, and an 8-acetylated hydroxylated metabolite. Proofs of structure were obtained by field desorption mass spectroscopy and 1H NMR, which indicated that the latter metabolite is probably substituted at ...
C, Fraire, P, Lecointe, C, Paoletti
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Ethidium Bromide Interaction with Poly(G)

Biophysical Reviews and Letters, 2014
In current work we investigate the interaction between polyguanylic acid and Ethidium Bromide ( EtBr ) and the changes of thermodynamic parameters due to those interactions by spectrophotometric methods. From the binding isotherms, binding constants were calculated for three different temperatures and the changes of Gibbs free energy, entropy and ...
Poghos H. Vardevanyan   +2 more
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Resistance to ethidium bromide inAspergillus nidulans

Experientia, 1979
A mutant ofAspergillus nidulans resistant to ethidium bromide was isolated and the semi-dominant gene responsible for this resistance was allocated on linkage group II at 17.42±3.05 units of recombination from thewA3 gene. The gene also confers cross-resistance to acriflavin, malachite green and crystal violet.
M. E. Scarazzatti   +2 more
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Binding of ethidium bromide to fractionated chromatin

Experimental Cell Research, 1976
Abstract The binding of ethidium bromide (EB) to different chromatin preparations was tested. Scatchard plots showed that the slowly sedimenting fraction of sheared chromatin is enriched in dye-binding sites. Limited nuclease digestion of rat liver nuclei, which has been shown to preserve the subunit structure of chromatin, reduces the number of ...
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Binding of ethidium bromide to avian erythrocyte chromatin

Biochemical and Biophysical Research Communications, 1972
Summary Ethidium bromide intercalation studies indicate that mature avian erythrocyte chromatin has 5.6 times fewer primary binding sites than deproteinized DNA. The number of both primary and secondary binding sites was increased upon removal of chromosomal proteins; among those, removal of avian erythrocyte specific histone V shows the highest ...
P F, Lurquin, V L, Seligy
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