Results 71 to 80 of about 246,567 (336)

Serotonergic System‐Targeted Nucleic Acid Hydrogel Coordinates Excitability Restoration and Circuit Reconstruction for Spinal Cord Injury Therapy

Advanced Materials, EarlyView.
The study reports a DNA/RNA heteroduplex hydrogel (SeroPTEN‐CG) that undergoes DNase‐mediated hydrogel‐to‐nanogel transition for spinal cord injury therapy by targeting the serotonergic system, combining 5‐hydroxytryptamine (5‐HT)‐mediated excitability restoration to reactivate dormant interneurons with phosphatase and tensin homolog (PTEN)‐targeted ...
Chunlin Li, Xiaoqing Zhao, Kai Jiang, Haixia Kang, Shuo Liu, Linlin Jiang, Baoshuai Bai, Haonan Cui, Ziyang Zhang, Menglei Dong, Ruizhi Zhang, Chenbo Zou, Shenghui Shang, Chi Zhang, Xiangchuang Fan, Lijuan Zhu, Huiquan Duan, Chuan Zhang, Shiqing Feng, Hengxing Zhou   +19 more
wiley   +1 more source

DNA extraction from formalin-fixed tissue: new light from the deep sea

Scientia Marina, 2010
DNA samples were extracted from ethanol and formalin-fixed decapod crustacean tissue using a new method based on Tetramethylsilane (TMS)-Chelex. It is shown that neither an indigestible matrix of cross-linked protein nor soluble PCR inhibitors impede PCR
Ferran Palero, Sally Hall, Paul F. Clark, David Johnston, Jackie Mackenzie-Dodds, Sven Thatje   +5 more
doaj   +1 more source

Nonmulberry Silk Fibroin Doping Boosts Charge Transfer and Charge Injection in Aligned Polypyrrole‐Silk Scaffolds for Low‐Voltage Neurostimulation

Advanced Materials Interfaces, EarlyView.
Conductive silk‐polypyrrole scaffolds couple redox stability with cell‐affinitive peptides present innately in an endogenous silk fibroin, enabling optimized electrical stimulation to drive neurite outgrowth. Findings establish electrochemical‐biological link for biomaterial design rules for smart nerve guidance conduits that can provide low voltage ...
Rajiv Borah, Joseph Christakiran Moses, Jnanendra Upadhyay, Jitu Mani Das, Michael G. Monaghan, Biman B. Mandal   +5 more
wiley   +1 more source

Phase‐Separation of YAP Mediates AJUBA Super Enhancer Activation to Promote Aberrant Mitosis in Breast Cancer

Advanced Science, EarlyView.
YAP undergoes phase separation to activate the AJUBA super‐enhancer, driving aberrant mitosis through spindle checkpoint dysregulation in breast cancer. This study uncovers a mechanistic link between YAP condensates, super‐enhancer activation, and aneuploidy formation.
Rui Zhang, Qingwen Huang, Zhuo Chen, Weijian Meng, Hongliang Dong, Zhihong Qi, Liang Liu, Jie Shen, Daxing Xie   +8 more
wiley   +1 more source

Investigating the Removal Ethidium Bromide from Aqueous Solutions using Activated Persulfate in Electrochemical Process

پژوهان, 2018
Background & Objectives: Ethidium bromide is known as a Mutagenic substance that due to some of its characteristics including accumulative property in organism bodies, can cause major environmental problems.
Mohammad Reza Samarghandi, Yousef Poureshgh, Mohammad Vanaei Tabar, Zahra Rahmani, Sattar Ahmadi   +4 more
doaj  

Zaleya decandra exerts anti-cancer activity in HCT-116 cancer cells by impairing mitochondrial activity

Bangladesh Journal of Pharmacology, 2019
An attempt has been made to investigate the role of Zaleya decandra on the survival of HCT colon cancer cells in vitro. The cell viability and apoptosis were assessed using MTT assay and acridine orange/ethidium bromide staining, respectively ...
Malarvizhi Deivasigamani, Ajay Kasivishwanathan Chandrasekar, Sridhar Muthusami, Gopalakrishnan Velliyur Kanniappan, Hariprasath Lakshmanan   +4 more
doaj   +1 more source

GC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps

Antibiotics, 2020
Considering the evidence that essential oils, as well as safrole, could modulate bacterial growth in different resistant strains, this study aims to characterize the phytochemical profile and evaluate the antibacterial and antibiotic-modulating ...
Ray S. Almeida, Priscilla R. Freitas, Ana Carolina J. Araújo, Irwin R. Alencar Menezes, Eduardo L. Santos, Saulo R. Tintino, Talysson F. Moura, Jaime R. Filho, Vitória A. Ferreira, Ana Cristina A. Silva, Luiz E. Silva, Wanderlei do Amaral, Cícero Deschamps, Marcello Iriti, Henrique D. Melo Coutinho   +14 more
doaj   +1 more source

CRISPR/Cas9-based editing of a sensitive transcriptional regulatory element to achieve cell type-specific knockdown of the NEMO scaffold protein [PDF]

, 2018
The use of alternative promoters for the cell type-specific expression of a given mRNA/protein is a common cell strategy. NEMO is a scaffold protein required for canonical NF-κB signaling.
Babaei, Milad, Gilmore, Thomas, Kagermazova, Larisa, Liu, Yuekun, Mccaslin, Ethan, Miyamoto, Shigeki, Wuerzberger-Davis, Shelly, Yeo, Alan   +7 more
core   +1 more source

Mammalian Proteome Profiling Reveals Readers and Antireaders of Strand‐Symmetric and ‐Asymmetric 5‐Hydroxymethylcytosine‐Modifications in DNA

Advanced Science, EarlyView.
ABSTRACT The cytosine (C) modifications 5‐methylcytosine (mC) and 5‐hydroxymethylcytosine (hmC) are central regulatory elements of mammalian genomes. Both marks occur in double‐stranded DNA in either strand‐symmetric or ‐asymmetric fashion, but it is still poorly understood how this symmetry information is selectively read out by the nuclear proteome ...
Lena Engelhard, Zeyneb Vildan Cakil, Marlon S. Zambrano‐Mila, Simone Eppmann, Tye Gonzalez, Rasmus Linser, Petra Janning, Sidney Becker, Daniel Summerer   +8 more
wiley   +1 more source

大腸菌を用いたフォスファカン（コンドロイチン硫酸プロテオグリカン）の融合コア蛋白の発現条件の検討 [PDF]

, 1996
Optimal conditions for expressing a specific region of core protein of phosphacan, a chondroitin sulfate proteoglycan known as receptor type protein tyrosine phosphatase, as fusion protein with glutathione S-transferase (GST) in E.coli were examined. DNA
Ito, Sekiko, Okamoto, Motoi
core   +1 more source