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Versatile biotechnological applications of Euglena gracilis
World Journal of Microbiology and Biotechnology, 2023Euglena gracilis is a freshwater protist possessing secondary chloroplasts of green algal origin. Various physical factors (e.g. UV) and chemical compounds (e.g. antibiotics) cause the bleaching of E. gracilis cells-the loss of plastid genes leading to the permanent inability to photosynthesize. Bleaching can be prevented by antimutagens (i.e.
Diana Lihanová +6 more
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Fully Deuterated Euglena gracilis
Science, 1964Euglena gracilis has been grown in 99.4 percent D 2 O on fully deuterated substrates. It is the first organism with distinct animal characteristics in which at least 99 percent of the hydrogen normally present has been replaced by deuterium.
S E, MANDEVILLE, H L, CRESPI, J J, KATZ
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A Euglena gracilis zinc endonuclease
Biochemistry, 1993A 26-kDa endonuclease has been purified to homogeneity from zinc-sufficient Euglena gracilis. The protein binds to single-stranded DNA with a higher affinity than to double-stranded DNA, but it exhibits nucleolytic activity toward both. Thus, it converts supercoiled plasmid pBR322 DNA into the linear form, a property characteristic of endonucleases ...
M, Czupryn +3 more
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Thiamin uptake in Euglena gracilis
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1987Thiamin uptake has been investigated in Euglena gracilis Z. This protozoon possessed an active transport system for thiamin with a Km value of 17 nM and a Vmax value of 7.8 pmol per 10(6) cells per min. Thiamin uptake was dependent on pH and temperature, but not on exogenous glucose as an energy source.
S, Shigeoka +4 more
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Ferredoxin biosynthesis in Euglena gracilis
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976Analysis of ferredoxin content in cultures of Euglena gracilis grown in the presence of selective antibiotic inhibitors of protein synthesis resulted in the following conclusions: 1. Ferredoxin is synthesized from cytoplasmic (80s-type) ribosomes; cycloheximide, a potent inhibitor of 80s translation completely abolished the synthesis, while the ...
R S, Matson, T, Kimura
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Respiratory cytochromes of Euglena gracilis
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1969Abstract 1. 1. Difference spectra of whole cells and of a particulate fraction of a streptomycin-bleached strain of Euglena gracilis showed the presence of a b -type cytochrome, cytochrome b (561 Euglena), and an a -type cytochrome, cytochrome a -type (609 Euglena). The cytochromes were characterized by pyridine hemochromogen formation and
J K, Raison, R M, Smillie
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Heteroxanthin in Euglena gracilis
Archiv f�r Mikrobiologie, 19731. From a large scale preparation of Euglena gracilis, strain Z, besides the acetylenic pigments diatoxanthin and diadinoxanthin and the allene neoxanthin, an additional acetylenic xanthophyll has been isolated. 2. Mass and IR spectra and chemical reactions showed typical patterns of heteroxanthin from Vaucheria. 3.
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Isolation of nuclei from Euglena gracilis
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969Abstract A method for the mass isolation of Euglena nuclei is described. Breakage of cells, without damaging the nuclei, is obtained by “loosing” the tough cell envelope by freezing and thawing and Triton treatment or by digestion with pancreatic protease prior to cell breakage.
F, Parenti +3 more
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Actin Gene Sequence from Euglena gracilis
Journal of Eukaryotic Microbiology, 1998ABSTRACT The full length coding sequence of the Euglena gracilis actin gene was determined by RT‐PCR of Euglena gracilis mRNA. Conserved regions in the actin amino acid sequence were used as guides for the synthesis of degenerate primers. Sequence was obtained for 1.238 nucleotides, of which 1.131 were coding for 377 amino acids.
S K, Petersen-Mahrt +2 more
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