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Selectable Markers for Eukaryotic Cells
2003The transfer of DNA sequences into a population of cells can rarely, if ever, be achieved with 100% efficiency. Typically, transfection of cells with the calcium phosphate method will transduce only between 0.1 and 1% of the cells with the sequences of interest (1), although some workers have achieved higher efficiencies (2).
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The protoplast of the eukaryotic cell
2005Perspective The eukaryotic cell is composed, with a few exceptions, of both a living protoplast, the site of cellular metabolism, and an enclosing cellulosic wall of one or more layers (Fig. 3.1). While not alive as a structural unit, the wall is commonly traversed by living components, plasmodesmata , which connect adjacent protoplasts and, thus ...
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Transactions of the American Microscopical Society, 1972
Lynn Margulis, John O. Corliss
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Lynn Margulis, John O. Corliss
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The Origin of Eukaryote and Archaebacterial Cells
Annals of the New York Academy of Sciences, 1987openaire +3 more sources