Results 121 to 130 of about 27,996,561 (298)

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Eukaryotic Initiation Factor 4G Suppresses Nonsense-Mediated mRNA Decay by Two Genetically Separable Mechanisms [PDF]

, 2014
Nonsense-mediated mRNA decay (NMD), which is best known for degrading mRNAs with premature termination codons (PTCs), is thought to be triggered by aberrant translation termination at stop codons located in an environment of the mRNP that is devoid of ...
Oliver Mühlemann (8914), Joncourt, Raphael, Raphael Joncourt (620344), Simone C Rufener, Song, Haiwei, Eberle, Andrea, Mühlemann, Oliver, Simone C. Rufener (620345), Raphael Joncourt, Andrea B Eberle, Rufener, Simone, Oliver Mühlemann, Andrea B. Eberle (245913)   +12 more
core   +2 more sources

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

A quantitative estimation of the global translational activity in logarithmically growing yeast cells. [PDF]

, 2008
BACKGROUND: Translation of messenger mRNAs makes significant contributions to the control of gene expression in all eukaryotes. Because translational control often involves fractional changes in translational activity, good quantitative descriptions of ...
von der Haar, Tobias, Tobias von der Haar   +1 more
core   +1 more source

Flavivirus Infection Uncouples Translation Suppression from Cellular Stress Responses

mBio, 2017
As obligate parasites, viruses strictly depend on host cell translation for the production of new progeny, yet infected cells also synthesize antiviral proteins to limit virus infection. Modulation of host cell translation therefore represents a frequent
Hanna Roth, Vera Magg, Fabian Uch, Pascal Mutz, Philipp Klein, Katharina Haneke, Volker Lohmann, Ralf Bartenschlager, Oliver T. Fackler, Nicolas Locker, Georg Stoecklin, Alessia Ruggieri   +11 more
doaj   +1 more source

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

FEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav, Shweta Shweta, Manisha Malhotra, Vikash Maurya, Akhilesh Kumar   +4 more
wiley   +1 more source

Human GINS, a conserved DNA replication factor and candidate cancer marker [PDF]

, 2011
The GINS complex (a heterotetramer of Sld5, Psf1, Psf2 and Psf3) is a highly conserved DNA replication factor required for the initiation and elongation of DNA replication. GINS is believed to associate with Cdc45 and MCM proteins on replicating DNA. The

core   +2 more sources

Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation

FEBS Open Bio, EarlyView.
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata, Yuxin Li, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source

A molecular stabiliser of an inhibitory eIF2B-eIF2(αP) complex activates the Integrated Stress Response

Nature Communications
Eukaryotic initiation factor 2B (eIF2B), a guanine nucleotide exchange factor (GEF), promotes protein synthesis by charging translation initiation factor 2 (eIF2) with GTP.
Fiona Shilliday, Miguel Gancedo-Rodrigo, Ginto George, Shintaro Aibara, Santosh Adhikari, Syedah Neha Ashraf, Evelyne J. Barrey, Paolo A. Centrella, Damian Crowther, Paige Dickson, Diana Gikunju, Marie-Aude Guié, John P. Guilinger, Anders Gunnarsson, Heather P. Harding, Christopher D. Hupp, Rachael Jetson, Anthony D. Keefe, JeeSoo Monica Kim, Richard J. Lewis, Taiana Maia de Oliveira, Jennifer Le-Marshall, Usha Narayanan, Katherine A. Nugai, Dušan Petrović, Emma Rivers, David Ron, Daisy Stringfellow, Karl Syson, Lewis Ward, John T. S. Yeoman, Yan Yu, Ying Zhang, Alisa Zyryanova, David J. Baker, Perla Breccia, John E. Linley   +36 more
doaj   +1 more source