Results 111 to 120 of about 27,996,561 (298)

Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region

FEBS Open Bio, EarlyView.
Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson, Anja H. Schiemann, Evelyn Sattlegger   +2 more
wiley   +1 more source

EIF3A (eukaryotic translation initiation factor 3, subunit A) [PDF]

, 2011
Review on EIF3A (eukaryotic translation initiation factor 3, subunit A), with data on DNA, on the protein encoded, and where the gene is ...
Zhang, JT, Yin, JY, Dong, Z
core   +1 more source

Isolation of eukaryotic initiation factor 2 from yeast Saccharomyces cerevisiae.

Journal of Biological Chemistry, 1981
Protein synthesis initiation factor eIF-2 has been isolated from the high speed supernatant fraction of the yeast Saccharomyces cerevisiae. This purification steps include ammonium sulfate fractionation, column chromatography on Sephacryl 300 and hydroxyapatite, and glycerol gradient sedimentation.
R A, Baan, P B, Keller, A E, Dahlberg
openaire   +2 more sources

Ionomycin suppresses cancer cell growth by disrupting mitochondrial transcription

FEBS Open Bio, EarlyView.
In this study, we identify a new function for the selective Ca2+ ionophore, ionomycin, as an inhibitor of mitochondrial transcription. Both total and nascent RNA analyses revealed that ionomycin treatment reduces the transcription of mitochondrial genes.
Lishen Wang, Mingqiang Deng, Dongmei He, Diqin Lai, Dongjun Liu, Dengyong Zhu, Zhenhua Zhang, Peng Tang, Chuanman Zhou, Menghui Yin, Xichen Bao   +10 more
wiley   +1 more source

Characterization of GTP and Aminoacyl-tRNA Binding to Eukaryotic Initiation Factor 2 and Elongation Factor 1

, 1991
Eukaryotic protein synthesis requires two protein factors which bind aminoacyl-tRNA in a GTP-dependent manner. One, initiation factor 2 (eIF-2), binds only the initiator Met-tRNAi and carries it to the 40S subunit of the ribosome.
Kinzy, Terri Goss
core   +2 more sources

Proteins of the Nucleolus of Dictyostelium discoideum: Nucleolar Compartmentalization, Targeting Sequences, Protein Translocations and Binding Partners

Cells, 2019
The nucleoli of Dictyostelium discoideum have a comparatively unique, non-canonical, localization adjacent to the inner nuclear membrane. The verified nucleolar proteins of this eukaryotic microbe are detailed while other potential proteins are ...
Danton H. O’Day
doaj   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

DENR-MCTS1 heterodimerization and tRNA recruitment are required for translation reinitiation.

PLoS Biology, 2018
The succession of molecular events leading to eukaryotic translation reinitiation-whereby ribosomes terminate translation of a short open reading frame (ORF), resume scanning, and then translate a second ORF on the same mRNA-is not well understood ...
Yasar Luqman Ahmed, Sibylle Schleich, Jonathan Bohlen, Nicolas Mandel, Bernd Simon, Irmgard Sinning, Aurelio A Teleman   +6 more
doaj   +1 more source

Eukaryotic initiation factor-2, gamma subunit, suppresses proliferation and regulates the cell cycle via the MAPK/ERK signaling pathway in acute myeloid leukemia. [PDF]

J Cancer Res Clin Oncol, 2021
Lu J, Chen S, Tan H, Huang Z, Li B, Liu L, Chen Y, Zeng X, Zou Y, Xu L.   +9 more
europepmc   +1 more source

Localization and function of a eukaryotic-initiation-factor-2-associated 67-kDa glycoprotein

World Journal of Biological Chemistry, 2010
To study the localization and function of a eukaryotic initiation factor 2 (eIF2α)-associated 67-kDa glycoprotein (p67).Immunofluorescence staining, (35)S-Met/Cys metabolic labeling, Western blotting analysis, sucrose gradient centrifugation and high speed centrifugation were used to determine the localization of proteins in transiently transfected COS-
openaire   +2 more sources