Results 91 to 100 of about 27,996,561 (298)

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Phenotype selection reveals coevolution of muscle glycogen and protein and PTEN as a gate keeper for the accretion of muscle mass in adult female mice. [PDF]

, 2012
We have investigated molecular mechanisms for muscle mass accretion in a non-inbred mouse model (DU6P mice) characterized by extreme muscle mass. This extreme muscle mass was developed during 138 generations of phenotype selection for high protein ...
Hammon Harald M., Bidlingmaier, Martin, Bernhard G Baumgartner, Andreas Hoeflich, Zeissler, Anja, Brenig Bertram, Binder Gerhard, Sawitzky, Mandy, Christ Bruno, Binder, Gerhard, Bidlingmaier Martin, Stock Peggy, Metzger, Friedrich, Christ, Bruno, Bertram Brenig, Renne Ulla, Bielohuby Maximilian, Cornelia C Metges, Zeissler Anja, Sawitzky Mandy, Barbara J M Stoehr, Hoeflich Andreas, Metges, Cornelia C., Solvig Görs, Anja Zeissler, Gerhard Binder, Brenig, Bertram, Stoehr, Barbara J. M., Fromm-Dornieden Carolin, Görs, Solvig, Bielohuby, Maximilian, Stock, Peggy, Hammon, Harald M., Hoeflich, Andreas, Stoehr Barbara J. M., Harald M Hammon, Fromm-Dornieden, Carolin, Friedrich Metzger, Langhammer, Martina, Martina Langhammer, Metges Cornelia C., Langhammer Martina, Martin Bidlingmaier, Renne, Ulla, Görs Solvig, Mandy Sawitzky, Peggy Stock, Maximilian Bielohuby, Baumgartner, Bernhard G., Ulla Renne, Carolin Fromm-Dornieden, Baumgartner Bernhard G., Bruno Christ, Metzger Friedrich   +53 more
core   +2 more sources

Translational control by messenger RNA competition for eukaryotic initiation factor 2.

Journal of Biological Chemistry, 1982
Translation of globin mRNA in a micrococcal nuclease-treated reticulocyte lysate was studied in the presence of increasing amounts of Mengovirus RNA, under conditions in which the number of translation initiation events remains constant as judged by the transfer of label from N-formyl[35S]methionyl-tRNAf into protein.
H, Rosen, G, Di Segni, R, Kaempfer
openaire   +2 more sources

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

An evolutionary ratchet leading to loss of elongation factors in eukaryotes [PDF]

, 2014
Background The GTPase eEF1A is the eukaryotic factor responsible for the essential, universal function of aminoacyl-tRNA delivery to the ribosome.
Kamenski, Piotr, Atkinson, Gemma C.,, Atkinson, Gemma C., Soosaar, Axel, Hauryliuk, Vasili,, Ivan Chicherin, Martin Carr, Tenson, Tanel,, Vasili Hauryliuk, Carr, Martin, Tanel Tenson, Kamenski, Piotr,, Kuzmenko, Anton, Hauryliuk, Vasili, Tenson, Tanel, Kuzmenko, Anton,, Chicherin, Ivan,, Carr, Martin,, Piotr Kamenski, Gemma C Atkinson, Soosaar, Axel,, Anton Kuzmenko, Lund University., Axel Soosaar, Chicherin, Ivan   +24 more
core   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Phosphorylation of tobacco eukaryotic translation initiation factor 4A upon pollen tube germination [PDF]

, 1998
Eukaryotic translation initiation factor eIF-4A is a member of the DEAD box family of RNA helicases and RNA-dependent ATPases, In tobacco, eIF-4A is encoded by a gene family with one isoform, eIF-4A8, being exclusively expressed in pollen.
op den Camp, Roel G. L., Kuhlemeier, Cris   +1 more
core   +3 more sources

Exploring the interaction dynamics of eukaryotic translation initiation factor 2

Biochemical Society Transactions
Eukaryotic translation initiation typically involves recruitment of the 43S ribosomal pre-initiation complex (PIC) to the 5′-end of the mRNA to form the 48S PIC, followed by scanning in search of a start codon in a favorable nucleotide complex. The start codon is recognized through base-pairing with the anticodon of the initiator Met-tRNAi.
openaire   +2 more sources

Indirect inactivation of eukaryotic initiation factor 2 in reticulocyte lysate by selenite.

Journal of Biological Chemistry, 1980
Addition of selenite to rabbit reticulocyte lysate produces a biphasic pattern of translational inhibition. Sucrose density gradient shows that the onset of translational inhibition is accompanied by decreased Met-tRNAf binding to 43 SN ribosomal subunits and loss of polysomes.
B, Safer, R, Jagus, D, Crouch
openaire   +2 more sources

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source