Results 151 to 160 of about 3,131 (178)
Some of the next articles are maybe not open access.
Cell death caused by hyper-expression of a secretory exoglucanase in Escherichia coli
Protein Expression and Purification, 2005Induced expression of a gene fusion between the ompA leader sequence and the Cellulomonas fimi cex gene encoding a secretory exoglucanase, Exg, engineered in the Tac-cassette excretion vector was lethal to Escherichia coli. An exponentially growing culture harboring the recombinant construct suffered slow growth and 99.9% of its cells died within 60 ...
W K R Wong, W K R Wong
exaly +4 more sources
Applied Microbiology and Biotechnology, 1995
CelS is the most abundant subunit and an exoglucanase component of the Clostridium thermocellum cellulosome, multicomponent cellulase complex. The product inhibition pattern of CelS was examined using purified recombinant CelS (rCelS) produced in Escherichia coli. The rCelS activity on cellopentaose was strongly inhibited by cellobiose.
K, Kruus +3 more
openaire +2 more sources
CelS is the most abundant subunit and an exoglucanase component of the Clostridium thermocellum cellulosome, multicomponent cellulase complex. The product inhibition pattern of CelS was examined using purified recombinant CelS (rCelS) produced in Escherichia coli. The rCelS activity on cellopentaose was strongly inhibited by cellobiose.
K, Kruus +3 more
openaire +2 more sources
An investigation of the mechanism of the Cellulomonas fimi exoglucanase
2010The exoglucanase from Cellulomonas fimi catalyses the hydrolysis of cellobiose units from the non-reducing terminus of cello-oligosaccharides with overall retention of anomeric configuration. Its mechanism of action is therefore thought to involve a double displacement reaction, involving as the first step, formation of a glycosyl-enzyme intermediate ...
openaire +1 more source
Induction, production, repression, and de-repression of exoglucanase synthesis in Aspergillus niger
Bioresource Technology, 2004The influence of carbon and nitrogen sources on the production of cellulases was investigated. The enzyme production was variable according to the carbon source. Levels of beta-cellobiohydrolase (CBH) were minimal in the presence of even low concentrations of glucose. Enzyme production was stimulated by other carbohydrates.
Atif, Hanif, Amber, Yasmeen, M I, Rajoka
openaire +2 more sources
Biomolecular Engineering, 2001
The major yeast exoglucanase (ExgIb) consists of a 408 amino acid polypeptide carrying two short N-linked oligosaccharides attached to asparagines 165 (Asn(165)) and 325 (Asn(325)). These oligosaccharides are very similar, in both length and composition, to those present in the vacuolar protease carboxypeptidase Y.
G, Larriba, R, Cueva
openaire +2 more sources
The major yeast exoglucanase (ExgIb) consists of a 408 amino acid polypeptide carrying two short N-linked oligosaccharides attached to asparagines 165 (Asn(165)) and 325 (Asn(325)). These oligosaccharides are very similar, in both length and composition, to those present in the vacuolar protease carboxypeptidase Y.
G, Larriba, R, Cueva
openaire +2 more sources
Yeast exoglucanases. Where redundancy implies necessity.
Archives of medical research, 1994Three exoglucanase genes have been described in Saccharomyces cerevisiae. The bulk of the exoglucanase (EXO) activity is encoded by the EXG1 gene, whose primary gene product is differentially glycosylated during its transit to the cell surface to yield three isoenzymes: EXOI, EXOII and EXOII1/2.
G, Larriba +3 more
openaire +1 more source
Archives of Microbiology, 1985
The rate of synthesis and secretion of exo-1-3-beta-glucanase activity closely paralleled the specific rate of growth in exponentially growing Saccharomyces cerevisiae cells in batch culture. When the stationary phase was reached both synthesis and secretion stopped.
I, Olivero, L M, Hernandez, G, Larriba
openaire +2 more sources
The rate of synthesis and secretion of exo-1-3-beta-glucanase activity closely paralleled the specific rate of growth in exponentially growing Saccharomyces cerevisiae cells in batch culture. When the stationary phase was reached both synthesis and secretion stopped.
I, Olivero, L M, Hernandez, G, Larriba
openaire +2 more sources
Hydrolase and transferase activities of the β‐1,3‐exoglucanase of Candida albicans
European Journal of Biochemistry, 1999The exo‐β‐1,3‐glucanase of Candida albicans (Exg) has a marked specificity for β‐1,3‐glucosidic linkages as judged by the kinetic constants for p‐nitophenyl β‐glucoside, β‐linked disaccharides of glucose (laminaribiose, gentiobiose, and cellobiose), oligosaccharides of the laminari series, laminarin and pustulan.
H J, Stubbs +3 more
openaire +2 more sources
Excretory production of a Cellulomonas fimi exoglucanase by Escherichia coli
2014An excretion reporter construct, tacIQpar8cex, was assembled by the cloning of the Celldomonas fimi exoglucanase gene, cex, into the TaC expression/excretion plasmid, lacIQpar8. E. coli JMlOl harbouring tacIQpar8cex was shown to produce extracellular exoglucanase (Exg) activity.
openaire +2 more sources
Over-production of the major exoglucanase of leads to an increase in the aroma of wine
International Journal of Food Microbiology, 2005Paloma Manzanares +2 more
exaly