Results 141 to 150 of about 14,025 (272)
Purification of a human polyribosome-associated 3‘ to 5‘ exoribonuclease. [PDF]
Journal of Biological Chemistry, 1994 In cells and cell-free extracts, the early steps in histone mRNA decay occur at the 3' terminus and appear to be catalyzed by a polysome-associated 3' to 5' exoribonuclease. We describe the purification of a polysomal 3' to 5' exoribonuclease that is magnesium-dependent, active at pH 7-8 in salt concentrations below 200 mM, and resistant to the ...
N Caruccio, Jeffrey Ross
openaire +2 more sources
Polynucleotide phosphorylase has an impact on cell biology of Campylobacter jejuni [PDF]
, 2012 Polynucleotide phosphorylase (PNPase), encoded by the pnp gene, is known to degrade mRNA, mediating post-transcriptional regulation and may affect cellular functions. The role of PNPase is pleiotropic. As orthologs of the two major ribonucleases (RNase E
Christopher M. Burns +7 more
core +1 more source
The Mysterious World of Non‐Canonical Caps – What We Know and Why We Need New Sequencing Techniques
ChemBioChem, Volume 26, Issue 3, February 1, 2025.This review describes sophisticated methodologies such as LC–MS that enabled the identification of 5’ non‐canonical RNA caps and also surveys so far developed sequencing methods, evaluating their advantages and disadvantages. It emphasizes the need for developing new, reliable sequencing techniques to identify RNA types with non‐canonical caps enabling
Flaminia Mancini, Hana Cahova
wiley +1 more source
Localisation of the human hSuv3p helicase in the mitochondrial matrix and its preferential unwinding of dsDNA [PDF]
, 2002 We characterised the human hSuv3p protein belonging to the family of NTPases/helicases. In yeast mitochondria the hSUV3 orthologue is a component of the degradosome complex and participates in mtRNA turnover and processing, while in Caenorhabditis ...
Awiszus, K. +10 more
core +2 more sources
mRNAs of plants and green algae lack the m7G cap‐1 structure
New Phytologist, Volume 246, Issue 2, Page 396-401, April 2025.
Chen Xiao +7 more
wiley +1 more source
Looping forward: exploring R‐loop processing and therapeutic potential
FEBS Letters, Volume 599, Issue 2, Page 244-266, January 2025.R‐loops function as both regulators of gene expression, DNA repair, telomere maintenance or chromosome segregation, and as drivers of genome instability. This dual nature of these structures is coordinated by RNA/DNA/chromatin processing factors that safeguard the genome from detrimental R‐loop‐instigated DNA damage or cellular dysfunction ...
Kalliopi Stratigi +2 more
wiley +1 more source
Adenovirus-mediated hPNPase(old-35) gene transfer as a therapeutic strategy for neuroblastoma [PDF]
, 2009 Current treatment options for neuroblastoma fail to eradicate the disease in the majority of high-risk patients, clearly mandating development of innovative therapeutic strategies. Gene therapy represents a promising approach for reversing the neoplastic
Ardelt +37 more
core +2 more sources
Frontiers in Plant Science, 2018 The cytosolic RNA exosome, a 3′→5′ exoribonuclease complex, contributes to mRNA degradation in eukaryotes, limiting the accumulation of poorly-translated, improperly translated, or aberrant mRNA species.
Jacob O. Brunkard +3 more
doaj +1 more source
Structural and biochemical insights into small RNA 3' end trimming by Arabidopsis SDN1. [PDF]
, 2018 A family of DEDDh 3'→5' exonucleases known as Small RNA Degrading Nucleases (SDNs) initiates the turnover of ARGONAUTE1 (AGO1)-bound microRNAs in Arabidopsis by trimming their 3' ends. Here, we report the crystal structure of Arabidopsis SDN1 (residues 2-
Cao, Chunyang +10 more
core +2 more sources
Nature Communications, 2020 DIS3L2-mediated decay (DMD) is a surveillance pathway for certain non-coding RNAs (ncRNAs) including ribosomal RNAs (rRNAs), transfer RNAs (tRNAs), small nuclear RNAs (snRNAs), and RMRP. While mutations in DIS3L2 are associated with Perlman syndrome, the
M. Pirouz +6 more
semanticscholar +1 more source